Title:
ENGINEERING PROBIOTIC FOR DEGRADING URIC ACID, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/151556
Kind Code:
A1
Abstract:
Provided are an engineering probiotic for degrading uric acid, and a construction method therefor and the use thereof, which belongs to the field of biotechnology. The probiotic Escherichia coli Nissle 1917 is used as an original starting strain, and an exogenous gene is introduced into a genome thereof by means of genetic engineering technology for modification, such that uric acid can be efficiently and rapidly degraded; and it is proved by tests that the rapid degradation of uric acid can be achieved in the intestinal tract and blood of mice. Compared with current lactic acid bacteria for degrading uric acid in the market, the constructed engineering probiotic has a stronger degradation ability and a better treatment effect, and therefore has a good practical application value.
Inventors:
XIA YONGZHEN (CN)
XUN LUYING (CN)
ZHAO RUI (CN)
XUN LUYING (CN)
ZHAO RUI (CN)
Application Number:
PCT/CN2023/074803
Publication Date:
August 17, 2023
Filing Date:
March 07, 2023
Export Citation:
Assignee:
UNIV SHANDONG (CN)
International Classes:
A61K48/00; C12N1/21; A61P19/06; C12N15/12; C12N15/53; C12N15/70; C12R1/19
Domestic Patent References:
| WO2021142491A2 | 2021-07-15 | |||
| WO2021173808A1 | 2021-09-02 |
Foreign References:
| CN114507631A | 2022-05-17 | |||
| CN101390969A | 2009-03-25 | |||
| CN111778224A | 2020-10-16 | |||
| CN110747157A | 2020-02-04 |
Other References:
LIXIA TANG, LIU JIE, LIU XIANJUN, JIANG XUE: "Construction and secetory expression of gene engineering bacteria of uric acid enzyme", JOURNAL OF CHONGQING MEDICAL UNIVERSITY., vol. 43, no. 1, 1 January 2018 (2018-01-01), pages 127 - 139, XP093084606, DOI: 10.13406/j.cnki.cyxb.000939
XIA PENGPENG, JUN ZHU, GUOQIANG ZHU: "Escherichia coli Nissle 1917 as safe vehicles for intestinal immune targeted therapy——A review", ACTA MICROBIOLOGICA SINICA., vol. 53, no. 6, 4 June 2013 (2013-06-04), pages 538 - 544, XP093084614, DOI: 10.13343/j.cnki.wsxb.2013.06.001
ZHAO RUI, LI ZIMAI, SUN YUQING, GE WEI, WANG MINGYU, LIU HUAIWEI, XUN LUYING, XIA YONGZHEN: "Engineered Escherichia coli Nissle 1917 with urate oxidase and an oxygen-recycling system for hyperuricemia treatment", GUT MICROBES, LANDES BIOSCIENCE, UNITED STATES, vol. 14, no. 1, 31 December 2022 (2022-12-31), United States , XP093084607, ISSN: 1949-0976, DOI: 10.1080/19490976.2022.2070391
XIA PENGPENG, JUN ZHU, GUOQIANG ZHU: "Escherichia coli Nissle 1917 as safe vehicles for intestinal immune targeted therapy——A review", ACTA MICROBIOLOGICA SINICA., vol. 53, no. 6, 4 June 2013 (2013-06-04), pages 538 - 544, XP093084614, DOI: 10.13343/j.cnki.wsxb.2013.06.001
ZHAO RUI, LI ZIMAI, SUN YUQING, GE WEI, WANG MINGYU, LIU HUAIWEI, XUN LUYING, XIA YONGZHEN: "Engineered Escherichia coli Nissle 1917 with urate oxidase and an oxygen-recycling system for hyperuricemia treatment", GUT MICROBES, LANDES BIOSCIENCE, UNITED STATES, vol. 14, no. 1, 31 December 2022 (2022-12-31), United States , XP093084607, ISSN: 1949-0976, DOI: 10.1080/19490976.2022.2070391
Attorney, Agent or Firm:
JINAN SHENGDA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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