Title:
ENGINEERING PROBIOTIC HAVING SURFACE DISPLAY PHENYLALANINE AMMONIA-LYASE
Document Type and Number:
WIPO Patent Application WO/2022/148362
Kind Code:
A1
Abstract:
Provided is an engineering probiotic having surface display phenylalanine ammonia-lyase. The engineering probiotic knocks out a gene argR and/or undergoes a gene argA(Y19C) mutation on an Escherichia coli Nissle 1917 genome, and integrates an L-phenylalanine ammonia-lyase gene stlA, an L-phenylalanine intracellular transport protein gene pheP, an L-amino acid deaminase gene pma, and an efflux pump gene acrA. The engineering probiotic can be used for treating phenylketonuria.
Inventors:
JIANG YU (CN)
Application Number:
PCT/CN2022/070241
Publication Date:
July 14, 2022
Filing Date:
January 05, 2022
Export Citation:
Assignee:
SHANGHAI TAOYUSHENG BIOTECHNOLOGY CO LTD (CN)
SUZHOU PREC BIOTECH CO LTD (CN)
SUZHOU PREC BIOTECH CO LTD (CN)
International Classes:
C12N1/21; A61K35/741; A61K38/51; A61P3/00; C12N15/70; C12R1/19
Domestic Patent References:
| WO2017087580A1 | 2017-05-26 | |||
| WO2004053111A1 | 2004-06-24 |
Foreign References:
| CN112662607A | 2021-04-16 | |||
| CN112662606A | 2021-04-16 | |||
| CN112501098A | 2021-03-16 | |||
| CN111655841A | 2020-09-11 | |||
| US20180320161A1 | 2018-11-08 | |||
| US20170312320A1 | 2017-11-02 | |||
| CN103468665A | 2013-12-25 |
Other References:
ZHANG LEI, XU XIAOHENG; ZHANG SIJIN: "Research progress on the treatment of phenylketonuria ", ZHONGGUO DANGDAI ERKE ZAZHI - CHINESE JOURNAL OF CONTEMPORARYPEDIATRICS, ZHONGNAN DAXUE,, CN, vol. 11, no. 9, 15 September 2009 (2009-09-15), CN , pages 786 - 789, XP055949676, ISSN: 1008-8830
ISABELLA VINCENT M; HA BINH N; CASTILLO MARY JOAN; LUBKOWICZ DAVID J; ROWE SARAH E; MILLET YVES A; ANDERSON CAMI L; LI NING; FISHE: "Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 36, no. 9, 1 October 2018 (2018-10-01), New York, pages 857 - 864, XP036929690, ISSN: 1087-0156, DOI: 10.1038/nbt.4222
ISABELLA VINCENT M; HA BINH N; CASTILLO MARY JOAN; LUBKOWICZ DAVID J; ROWE SARAH E; MILLET YVES A; ANDERSON CAMI L; LI NING; FISHE: "Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 36, no. 9, 1 October 2018 (2018-10-01), New York, pages 857 - 864, XP036929690, ISSN: 1087-0156, DOI: 10.1038/nbt.4222
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (CN)
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