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Title:
ENGINEERING SURFACE EPITOPES TO IMPROVE PROTEIN CRYSTALLIZATION
Document Type and Number:
WIPO Patent Application WO/2014/063098
Kind Code:
A3
Abstract:
The invention provides for methods and systems for engineering target proteins, based on protein sequence characteristics that influence the likelihood of obtaining a crystal suitable for X-ray structure solution, to improve protein crystallization, as well as related material.

Inventors:
NAUMOV, Victor (40 W. 116th Street, B607New York, NY, 10026, US)
PRICE, William, Nicholson, II (21800 Beallsville Rd, PO Box 68Barnesville, MD, 20838, US)
HANDELMAN, Samuel, K. (135 W Hubbard Avenue, Columbus, OH, 43215, US)
HUNT, John, Francis, III (924 West End Avenue, Apt 34New York, NY, 10025, US)
Application Number:
US2013/065748
Publication Date:
June 19, 2014
Filing Date:
October 18, 2013
Export Citation:
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Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK (412 Low Memorial Library, 535 West 116th StreetNew York, NY, 10027, US)
NAUMOV, Victor (40 W. 116th Street, B607New York, NY, 10026, US)
PRICE, William, Nicholson, II (21800 Beallsville Rd, PO Box 68Barnesville, MD, 20838, US)
HANDELMAN, Samuel, K. (135 W Hubbard Avenue, Columbus, OH, 43215, US)
HUNT, John, Francis, III (924 West End Avenue, Apt 34New York, NY, 10025, US)
International Classes:
C07K1/00; G01N31/00
Domestic Patent References:
WO2011133608A22011-10-27
Foreign References:
US20110033894A12011-02-10
US20120100593A12012-04-26
US20110031438A12011-02-10
Other References:
GOLDSCHMIDT ET AL.: "Toward rational protein crystallization: A Web server for the design of crystallizable protein variants.", PROTEIN SCI., vol. 16, no. 8, 2007, pages 1569 - 76
MIZIANTY ET AL.: "CRYSpred: accurate sequence-based protein crystallization propensity prediction using sequence-derived structural characteristics.", PROTEIN PEPT LETT., vol. 19, no. 1, January 2012 (2012-01-01), pages 40 - 9
KURGAN ET AL.: "Sequence-Based Protein Crystallization Propensity Prediction for Structural Genomics : Review and Comparative Analysis.", NATURAL SCIENCE, vol. 1, no. 2, 2009, pages 93 - 1 06; PG 96-101
"Baresic. Structural analysis of single amino acid polymorphisms.", PHD THESIS, 3 March 2012 (2012-03-03), pages 47 - 75, 85-114, Retrieved from the Internet [retrieved on 20140218]
DEREWENDA.: "Application of protein engineering to enhance crystallizability and improve crystal properties.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 66, 2010, pages 604 - 15
RUGGIERO ET AL.: "Enhanced crystallizability by protein engineering approaches: a general overview.", PROTEIN PEPT LETT., vol. 19, no. 7, July 2012 (2012-07-01), pages 732 - 42
MATEJA ET AL.: "The impact of Glu-->Ala and Glu-->Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 A resolution.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 58, 2002, pages 1983 - 91
WINE ET AL.: "Modification of protein crystal packing by systematic mutations of surface residues: implications on biotemplating and crystal porosity.", BIOTECHNOL BIOENG., vol. 104, no. 3, 2009, pages 444 - 57
PRICE II ET AL.: "Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data.", NAT BIOTECHNOL., vol. 27, no. 1, 2009, pages 51 - 7
COOPER ET AL.: "Protein crystallization by surface entropy reduction: optimization of the SER strategy.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 63, 2007, pages 636 - 45
Attorney, Agent or Firm:
ESTRADA DE MARTIN, Paula et al. (Wilmer Cutler Pickering Hale and Dorr LLP, 7 World Trade Center250 Greenwich Stree, New York NY, 10007, US)
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