Title:
ENGINEERING SURFACE EPITOPES TO IMPROVE PROTEIN CRYSTALLIZATION
Document Type and Number:
WIPO Patent Application WO/2014/063098
Kind Code:
A3
Abstract:
The invention provides for methods and systems for engineering target proteins, based on protein sequence characteristics that influence the likelihood of obtaining a crystal suitable for X-ray structure solution, to improve protein crystallization, as well as related material.
Inventors:
NAUMOV VICTOR (US)
PRICE WILLIAM NICHOLSON II (US)
HANDELMAN SAMUEL K (US)
HUNT JOHN FRANCIS III (US)
PRICE WILLIAM NICHOLSON II (US)
HANDELMAN SAMUEL K (US)
HUNT JOHN FRANCIS III (US)
Application Number:
PCT/US2013/065748
Publication Date:
June 19, 2014
Filing Date:
October 18, 2013
Export Citation:
Assignee:
UNIV COLUMBIA (US)
NAUMOV VICTOR (US)
PRICE WILLIAM NICHOLSON II (US)
HANDELMAN SAMUEL K (US)
HUNT JOHN FRANCIS III (US)
NAUMOV VICTOR (US)
PRICE WILLIAM NICHOLSON II (US)
HANDELMAN SAMUEL K (US)
HUNT JOHN FRANCIS III (US)
International Classes:
C07K1/00; G16B15/00; G01N31/00
Domestic Patent References:
| WO2011133608A2 | 2011-10-27 |
Foreign References:
| US20110033894A1 | 2011-02-10 | |||
| US20120100593A1 | 2012-04-26 | |||
| US20110031438A1 | 2011-02-10 |
Other References:
GOLDSCHMIDT ET AL.: "Toward rational protein crystallization: A Web server for the design of crystallizable protein variants.", PROTEIN SCI., vol. 16, no. 8, 2007, pages 1569 - 76
MIZIANTY ET AL.: "CRYSpred: accurate sequence-based protein crystallization propensity prediction using sequence-derived structural characteristics.", PROTEIN PEPT LETT., vol. 19, no. 1, January 2012 (2012-01-01), pages 40 - 9
KURGAN ET AL.: "Sequence-Based Protein Crystallization Propensity Prediction for Structural Genomics : Review and Comparative Analysis.", NATURAL SCIENCE, vol. 1, no. 2, 2009, pages 93 - 1 06; PG 96-101
"Baresic. Structural analysis of single amino acid polymorphisms.", PHD THESIS, 3 March 2012 (2012-03-03), pages 47 - 75, 85-114, Retrieved from the Internet [retrieved on 20140218]
DEREWENDA.: "Application of protein engineering to enhance crystallizability and improve crystal properties.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 66, 2010, pages 604 - 15
RUGGIERO ET AL.: "Enhanced crystallizability by protein engineering approaches: a general overview.", PROTEIN PEPT LETT., vol. 19, no. 7, July 2012 (2012-07-01), pages 732 - 42
MATEJA ET AL.: "The impact of Glu-->Ala and Glu-->Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 A resolution.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 58, 2002, pages 1983 - 91
WINE ET AL.: "Modification of protein crystal packing by systematic mutations of surface residues: implications on biotemplating and crystal porosity.", BIOTECHNOL BIOENG., vol. 104, no. 3, 2009, pages 444 - 57
PRICE II ET AL.: "Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data.", NAT BIOTECHNOL., vol. 27, no. 1, 2009, pages 51 - 7
COOPER ET AL.: "Protein crystallization by surface entropy reduction: optimization of the SER strategy.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 63, 2007, pages 636 - 45
MIZIANTY ET AL.: "CRYSpred: accurate sequence-based protein crystallization propensity prediction using sequence-derived structural characteristics.", PROTEIN PEPT LETT., vol. 19, no. 1, January 2012 (2012-01-01), pages 40 - 9
KURGAN ET AL.: "Sequence-Based Protein Crystallization Propensity Prediction for Structural Genomics : Review and Comparative Analysis.", NATURAL SCIENCE, vol. 1, no. 2, 2009, pages 93 - 1 06; PG 96-101
"Baresic. Structural analysis of single amino acid polymorphisms.", PHD THESIS, 3 March 2012 (2012-03-03), pages 47 - 75, 85-114, Retrieved from the Internet
DEREWENDA.: "Application of protein engineering to enhance crystallizability and improve crystal properties.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 66, 2010, pages 604 - 15
RUGGIERO ET AL.: "Enhanced crystallizability by protein engineering approaches: a general overview.", PROTEIN PEPT LETT., vol. 19, no. 7, July 2012 (2012-07-01), pages 732 - 42
MATEJA ET AL.: "The impact of Glu-->Ala and Glu-->Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 A resolution.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 58, 2002, pages 1983 - 91
WINE ET AL.: "Modification of protein crystal packing by systematic mutations of surface residues: implications on biotemplating and crystal porosity.", BIOTECHNOL BIOENG., vol. 104, no. 3, 2009, pages 444 - 57
PRICE II ET AL.: "Understanding the physical properties that control protein crystallization by analysis of large-scale experimental data.", NAT BIOTECHNOL., vol. 27, no. 1, 2009, pages 51 - 7
COOPER ET AL.: "Protein crystallization by surface entropy reduction: optimization of the SER strategy.", ACTA CRYSTALLOGR D BIOL CRYSTALLOGR., vol. 63, 2007, pages 636 - 45
Attorney, Agent or Firm:
ESTRADA DE MARTIN, Paula et al. (7 World Trade Center250 Greenwich Stree, New York NY, US)
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