Title:
ENGINEERING YEAST STRAIN FOR PRODUCING HYALURONIDASE AT HIGH YIELD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/056891
Kind Code:
A1
Abstract:
The present invention provides an engineering yeast strain for producing hyaluronidase at high yield. The engineering yeast strain is obtained by respectively integrating a hyaluronidase gene as represented by SEQ ID NO.1 into any two sites of a yeast genome by means of a genome-integrating vector. According to the present invention, the engineering yeast strain for producing hyaluronidase at high yield is constructed by means of an integrative plasmid-mediated recombinant bacteria construction method, the highest enzyme activity of the engineering yeast strain in a culture medium taking methanol as a carbon source is 5.9×105 U/mL, and the highest enzyme activity in a culture medium taking glycerol as a carbon source is 3.6×105 U/mL. Therefore, the present invention is beneficial to further lowering the cost of industrial production of Odontomachus monticola Emery hyaluronidase, so as to expand the application scope of hyaluronidase in the field of medicines, chemical industry, and food.
Inventors:
WANG HAO (CN)
XU RONGRONG (CN)
ZHENG QINGFANG (CN)
ZHANG MENG (CN)
ZHANG YOUHENG (CN)
ZHANG TIANMENG (CN)
GUO XUEPING (CN)
XU RONGRONG (CN)
ZHENG QINGFANG (CN)
ZHANG MENG (CN)
ZHANG YOUHENG (CN)
ZHANG TIANMENG (CN)
GUO XUEPING (CN)
Application Number:
PCT/CN2022/123093
Publication Date:
April 13, 2023
Filing Date:
September 30, 2022
Export Citation:
Assignee:
BLOOMAGE BIOTECHNOLOGY CORP LTD (CN)
International Classes:
C12N1/19; C12N9/26; C12N15/81; C12R1/84
Domestic Patent References:
| WO2022183541A1 | 2022-09-09 | |||
| WO2022183542A1 | 2022-09-09 |
Foreign References:
| CN114350535A | 2022-04-15 | |||
| CN114350639A | 2022-04-15 | |||
| CN114350691A | 2022-04-15 | |||
| CN114350728A | 2022-04-15 | |||
| CN108220272A | 2018-06-29 | |||
| CN104263707A | 2015-01-07 | |||
| CN103614352A | 2014-03-05 | |||
| CN102911887A | 2013-02-06 | |||
| CN103436508A | 2013-12-11 |
Other References:
DATABASE Nucleotide 5 February 2019 (2019-02-05), ANONYMOUS : "TSA: Odontomachus monticola mRNA, Om13456_c0_g1_i1, mRNA sequence", XP093056164, retrieved from ncbi Database accession no. FX985505.1
钊倩倩 等 (ZHAO, QIANQIAN ET AL.): "牛透明质酸酶PH-20核心区在毕赤酵母中的高效表达 (High-level Expression of Core-domain Coding Sequence of Hyaluronidase PH-20 from Bull Testis in Pichia Pastoris)", 食品与药品 (FOOD AND DRUG), vol. 15, no. 5, 31 December 2013 (2013-12-31), XP055963342, ISSN: 1672-979X
TANI NAOKI, KAZUMA KOHEI, OHTSUKA YUKIO, SHIGERI YASUSHI, MASUKO KEIICHI, KONNO KATSUHIRO, INAGAKI HIDETOSHI: "Mass Spectrometry Analysis and Biological Characterization of the Predatory Ant Odontomachus monticola Venom and Venom Sac Components", TOXINS, vol. 11, no. 1, 17 January 2019 (2019-01-17), pages 50, XP093056159, DOI: 10.3390/toxins11010050
KAZUMA KOHEI, MASUKO KEIICHI, KONNO KATSUHIRO, INAGAKI HIDETOSHI: "Combined Venom Gland Transcriptomic and Venom Peptidomic Analysis of the Predatory Ant Odontomachus monticola", TOXINS, vol. 9, no. 10, 13 October 2017 (2017-10-13), pages 323, XP055963343, DOI: 10.3390/toxins9100323
钊倩倩 等 (ZHAO, QIANQIAN ET AL.): "牛透明质酸酶PH-20核心区在毕赤酵母中的高效表达 (High-level Expression of Core-domain Coding Sequence of Hyaluronidase PH-20 from Bull Testis in Pichia Pastoris)", 食品与药品 (FOOD AND DRUG), vol. 15, no. 5, 31 December 2013 (2013-12-31), XP055963342, ISSN: 1672-979X
TANI NAOKI, KAZUMA KOHEI, OHTSUKA YUKIO, SHIGERI YASUSHI, MASUKO KEIICHI, KONNO KATSUHIRO, INAGAKI HIDETOSHI: "Mass Spectrometry Analysis and Biological Characterization of the Predatory Ant Odontomachus monticola Venom and Venom Sac Components", TOXINS, vol. 11, no. 1, 17 January 2019 (2019-01-17), pages 50, XP093056159, DOI: 10.3390/toxins11010050
KAZUMA KOHEI, MASUKO KEIICHI, KONNO KATSUHIRO, INAGAKI HIDETOSHI: "Combined Venom Gland Transcriptomic and Venom Peptidomic Analysis of the Predatory Ant Odontomachus monticola", TOXINS, vol. 9, no. 10, 13 October 2017 (2017-10-13), pages 323, XP055963343, DOI: 10.3390/toxins9100323
Attorney, Agent or Firm:
BEIJING TANGSONG IP FIRM (CN)
Download PDF:
Previous Patent: HIGH-ENTROPY ALLOY COATING, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Next Patent: TECHNICAL STAINS COMPRISING DNA
Next Patent: TECHNICAL STAINS COMPRISING DNA