Title:
ENHANCED-ACTIVITY MODIFIED BETA-GLUCOSIDASE AND A MANUFACTURING METHOD FOR BIOETHANOL USING SAME
Document Type and Number:
WIPO Patent Application WO/2012/141547
Kind Code:
A2
Abstract:
The present invention relates to a beta-glucosidase which has been modified so as to enhance the activity thereof, and to a production method for bioethanol using same. More specifically, the present invention relates to: a polynucleotide coding for a beta-glucosidase which has been modified so as to enhance the activity thereof; beta-glucosidase expressed from the polynucleotide; an expression vector comprising the polynucleotide; a transformant genetically transformed by means of the expression vector; a method for producing the modified beta-glucosidase by using the transformant; and a method for producing bioethanol by using the transformant. The modified beta-glucosidase of the present invention can be used to implement a saccharification process which is even more efficient than with existing beta-glucosidase, and can therefore be widely put to use in the economic production of bioethanol.
Inventors:
SOHN JUNG HOON (KR)
KIM HYUN JIN (KR)
KIM CHUL HO (KR)
SEOMOON JI HUN (KR)
SUNG BONG HYUN (KR)
LIM KWANG MOOK (KR)
KIM MI JIN (KR)
KIM JONG HYUN (KR)
KIM HYUN JIN (KR)
KIM CHUL HO (KR)
SEOMOON JI HUN (KR)
SUNG BONG HYUN (KR)
LIM KWANG MOOK (KR)
KIM MI JIN (KR)
KIM JONG HYUN (KR)
Application Number:
PCT/KR2012/002846
Publication Date:
October 18, 2012
Filing Date:
April 13, 2012
Export Citation:
Assignee:
KOREA RES INST OF BIOSCIENCE (KR)
SOHN JUNG HOON (KR)
KIM HYUN JIN (KR)
KIM CHUL HO (KR)
SEOMOON JI HUN (KR)
SUNG BONG HYUN (KR)
LIM KWANG MOOK (KR)
KIM MI JIN (KR)
KIM JONG HYUN (KR)
SOHN JUNG HOON (KR)
KIM HYUN JIN (KR)
KIM CHUL HO (KR)
SEOMOON JI HUN (KR)
SUNG BONG HYUN (KR)
LIM KWANG MOOK (KR)
KIM MI JIN (KR)
KIM JONG HYUN (KR)
International Classes:
C12N15/56; C12N9/24; C12N15/31; C12P7/06
Domestic Patent References:
| WO2008155665A2 | 2008-12-24 |
Foreign References:
| KR20100091289A | 2010-08-19 | |||
| KR20100131208A | 2010-12-15 | |||
| KR20110007981A | 2011-01-25 | |||
| KR100697310B1 | 2007-03-20 |
Other References:
WENJIN LIU ET AL.: 'Fast Identification of Thermostable Beta-Glucosidase Mutants on Cellobiose by a Novel Combinatorial Selection/Screening Approach' BIOTECHNOLOGY AND BIOENGINEERING vol. 103, no. 6, 15 August 2009, pages 1087 - 1094
Attorney, Agent or Firm:
SON, MIN (KR)
손민 (KR)
손민 (KR)
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Claims: