Title:
ENHANCED CELLULAR ASSAY METHOD FOR USE IN FLOW CYTOMETRY OR SIMILAR INSTRUMENTS USING OPTICALLY RESONANT PARTICLES
Document Type and Number:
WIPO Patent Application WO2004106535
Kind Code:
A3
Abstract:
This invention relates to the field of biological assays where cells can be classified and enumerated using flow cytometry optical instrumentation. The invention combines information from multi-angle, light scatter from the cell itself and multi-angle light scatter from small, optically resonant particles that are selectively bound to surface molecules on the cell to carry out classification and enumeration. This light scatter method enables an instrumentation system that is simple to use, inexpensive to build, and mechanically robust; making it suitable for use in remote clinical environments.
Inventors:
HANSEN PETER W (US)
KRAULEDAT PETRA B (US)
KRAULEDAT PETRA B (US)
Application Number:
PCT/US2004/016855
Publication Date:
October 26, 2006
Filing Date:
May 27, 2004
Export Citation:
Assignee:
POINTCARE TECHNOLOGIES INC (US)
HANSEN PETER W (US)
KRAULEDAT PETRA B (US)
HANSEN PETER W (US)
KRAULEDAT PETRA B (US)
International Classes:
G01N33/553; G01N15/14; G01N33/58; G01N15/00
Foreign References:
| US6413786B1 | 2002-07-02 | |||
| US5939021A | 1999-08-17 | |||
| US4284412A | 1981-08-18 |
Other References:
SIIMAN O.: "Preparation, Microscopy, and Flow Cytometry with Excitation into Surface Plasmon Resonance Bands of Gold or Silver Nanoparticles on Aminodextran-Coated Polystyrene Beads", J. PHYS. CHEM. B, vol. 104, 2000, pages 9795 - 9810, XP008071161
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