Title:
ENHANCED MONOMERIC STAYGOLD PROTEIN AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/032020
Kind Code:
A1
Abstract:
The present invention relates to the field of recombinant protein technology, and particularly, to an enhanced monomeric StayGold protein and use thereof. The sequence of the enhanced monomeric StayGold protein is a mutant sequence of the wildtype dimeric StayGold protein, and the mutation in the mutant sequence is a mutation of at least one amino acid of N137, Q140 and Y187 to any amino acid. The novel monomeric StayGold protein, abbreviated as mStayGold, is a green fluorescent protein having high thermal stability and fluorescence intensity. The Tm of the protein is about 95 °C, which is 15 °C higher than the Tm of a common EGFP, suggesting comparable thermal stability to that of the dimeric StayGold protein. The protein also features a yield of 3.5 times greater and a fluorescence intensity of 6 times greater than those of a common EGFP. The properties of the monomer solve the problem that the labeling and location functionality of the wildtype dimeric protein may be affected by the aggregation of the protein when the protein is fused with a target protein for tracking and locating the target protein, and thereby possesses better application prospects in the application of guiding protein expression, cell location, and the like.
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Inventors:
WANG FENG (CN)
WU JIAQUAN (CN)
CHEN QIAN (CN)
YANG YIHU (CN)
GUI WENJUN (CN)
WU JIAQUAN (CN)
CHEN QIAN (CN)
YANG YIHU (CN)
GUI WENJUN (CN)
Application Number:
PCT/CN2023/089027
Publication Date:
February 15, 2024
Filing Date:
April 18, 2023
Export Citation:
Assignee:
BIORTUS BIOSCIENCE CO LTD (CN)
International Classes:
C12N15/11; C07K14/00; C12N15/62; C12Q1/68
Foreign References:
| CN105585625A | 2016-05-18 | |||
| CN1438239A | 2003-08-27 | |||
| US20050130262A1 | 2005-06-16 | |||
| US20110126305A1 | 2011-05-26 | |||
| US5625048A | 1997-04-29 |
Other References:
HIRANO, M. ET AL.: "A highly photostable and bright green fluorescent protein.", NATURE BIOTECHNOLOGY, vol. 40, 25 April 2022 (2022-04-25), XP037903041, DOI: 10.1038/s41587-022-01278-2
REICHEL, C. ET AL.: "Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells.", PROC.NATL.ACAD.SCI.USA, vol. 93, 30 June 1996 (1996-06-30), XP002056949, DOI: 10.1073/pnas.93.12.5888
DATABASE Protein 18 June 2022 (2022-06-18), ANONYMOUS : "fluorescent protein [synthetic construct]", XP093137760, retrieved from NCBI Database accession no. BCN13401.1
REICHEL, C. ET AL.: "Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells.", PROC.NATL.ACAD.SCI.USA, vol. 93, 30 June 1996 (1996-06-30), XP002056949, DOI: 10.1073/pnas.93.12.5888
DATABASE Protein 18 June 2022 (2022-06-18), ANONYMOUS : "fluorescent protein [synthetic construct]", XP093137760, retrieved from NCBI Database accession no. BCN13401.1
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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