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Title:
ENHANCED NATURAL COLORS
Document Type and Number:
WIPO Patent Application WO/2012/009469
Kind Code:
A2
Abstract:
A natural color is concentrated to intensify color range and to provide useful amounts of one or more of anti-oxidant, nutritional, and anti-inflammatory compounds derived from one or more pigment sources. In a preferred embodiment, the pigment source is a fruit, a vegetable, a legume, a spice, algae, or a combination thereof.

Inventors:
ALTAFFER PAUL (US)
WUAGNEUX JEFFREY M (US)
HSU PI-YU (US)
Application Number:
PCT/US2011/043904
Publication Date:
January 19, 2012
Filing Date:
July 13, 2011
Export Citation:
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Assignee:
RFI LLC (US)
ALTAFFER PAUL (US)
WUAGNEUX JEFFREY M (US)
HSU PI-YU (US)
International Classes:
C09B61/00; C09B67/00
Foreign References:
US4302200A1981-11-24
Other References:
See references of EP 2593516A4
Attorney, Agent or Firm:
BOYS, Donald, R. (Suite DWatsonville, CA, US)
Download PDF:
Claims:
What is claimed is:

1. A natural color concentrated, intensifying color range and providing enhanced levels of one or more of anti-oxidant, nutritional, and anti-inflammatory compounds, the natural color derived from one or more pigment sources.

2. The natural color of claim 1, wherein the pigment source is a fruit, a vegetable, a legume, a spice, an algae, or a combination thereof. 3. The natural color of claim 1, wherein the pigment or pigments in the color are extracted from or concentrated from one or a combination of grape, beet, red cabbage, red radish, hibiscus, carmine, red sandalwood, purple carrot, black carrot, purple sweet potato, purple corn, black currant, bilberry, elderberry, maqui berry, natural carotenoids, carrot, turmeric, curcumin, paprika, annatto, lutein, marigold, spinach, chlorophyll, and spirulina.

4. The natural color of claim 1, wherein the pigment or pigments in the color are extracted by one of or a combination of screw press, hydraulic press, juicing, natural solvent extraction, synthetic solvent extraction, and distillation.

5. The natural color of claim 4, further processed by one or a combination of vacuum concentration, steam concentration, supercritical carbon dioxide extraction, distillation, ultra-filtration, membrane filtration, column purification, and ion exchange.

6. The natural color of claim 1, wherein the color compound is dried using one or a combination of spray drying, vacuum drying, drum drying, refractance window drying, radiant zone drying and freeze drying. 7. The natural color of claim 1, wherein the color compound is endogenous.

8. The natural color of claim 1, wherein the color compound is exogenous.

9. The natural color of claim 7, wherein the color compound includes one or more of vitamins, minerals, fats, proteins, and sugars. 10. The natural color of claim 8, wherein the color compound includes one or a combination of rosemary, butylated hydroxytoulene (BHT), citrus oils, citric acid, and potassium sorbate.

11. The natural color of claim 8, wherein the color compound includes one or more of the compounds co-enzyme Q10 (CoQIO), resveratrol, statins, phytosterols, and dietary fiber.

12. The natural color of claim 8, wherein the color compound includes one or more of polysaccharides, methylxanthine, caffeine, theobromine and theophylline.

13. The natural color of claim 8, wherein the color compound includes one or more of 1-thiamine, 1-arginine, 1-phenylalanine, 1-tryptophan, rhodiola, and rosea.

14. The natural color of claim 8, wherein the natural color includes one or more of omega 3 fatty acids, docosahexanoic acid (DHA), eicosapentaenoic acid (EPA), phosphatidyl choline, phosphatidyl serine, and gingko biloba.

Description:
ENHANCED NATURAL COLORS

CROSS-REFERENCE TO RELATED DOCUMENTS The present invention claims priority to a U.S. provisional patent application serial numbers 61/410,621, filed 11/05/2010, and 61/363,830, filed 07/13/2010, both of which are entitled "Enhanced Natural Colors That Provide Color and Nutritional Properties". The specification also includes accompanying appendices A, B, C and D. BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention is in the field of processing natural pigments and pertains particularly to methods and apparatus for concentrating and enhancing natural pigments to include useful amounts of anti-oxidants and anti-inflammatory agents.

2. Discussion of the State of the Art

Various home -based and commercial processes exist for generating natural pigments from organic materials including fruits, vegetables, legumes, and spices. Natural pigmentation is used in state-of-art processes related to the generation of compounds that include the natural pigment as a desired color. Artificial coloring has been used extensively in a wide variety of products, however natural pigments are gaining recognition as being a healthier alternative to artificial coloring.

Oxidative Stress and Inflammation are two of the most important markers for disease states and are associated with many illnesses and dysfunctions. Anti-oxidants and nutrients are therefore important regimens in disease treatment and prevention.

Natural colors are comprised mostly of classes of ingredients considered to be antioxidants. Products with natural pigments include food and beverage products, dietary supplements, pharmaceuticals, skin care and cosmetics, and similar compounds that require some form of pigmentation in the mix of compounds and ingredients. Current state-of-art processes for generating these natural pigments focus on preserving the color in the compound as opposed to preserving amounts of nutrients and/or anti-oxidative components that may also be found in the pigment source.

A limitation with current natural pigment processing techniques is that generating the color is the primary focus of generating the compounds. As a result, consumer products bearing natural pigments have nutritional and anti-oxidation properties that are not useful in the compound because the concentration levels are too low. These natural pigment compounds would need to be consumed at a very high rate in order that nutritional and anti-oxidant properties have any positive effect on the consumer. It would be desired that natural pigments used in consumer products include useful levels of the nutrients and anti-oxidants that are generic to the pigment sources.

Therefore, what is clearly needed is a set of natural colors that include higher or useful levels of source-associated and anti-oxidants and nutrients while preserving, and in many cases, improving the intensity of the associated color, as well as preserve the functionality as a color, like flavor and aroma neutrality.

SUMMARY OF THE INVENTION The problem stated above is that nutritional, anti-inflammatory, and anti- oxidative value is desirable for natural color compounds, but many of the

conventional means for extracting natural colors, such as a screw press, only focus on obtaining the color. The inventors discovered that by using various methods for extraction, purification, and concentration, these natural color compounds could be enhanced significantly to include useful amounts of vitamins, nutrients, antioxidants, and anti-inflammatory elements and compounds.

The present inventor realized in a moment of discovery that, at the point of extraction, natural color compounds could be caused to exhibit useful amounts of anti-oxidants, anti-inflammatory compounds, and nutrients either derived from the source materials or caused to be retained within the compound from an external source separate from the original compound. The nutritive compounds can be concentrated to a point where they exert a function beyond color, into the area of human nutrition, while preserving the functional properties of the natural color.

The invention discloses processes and actual product examples that accomplish both goals of preserving functional color and nutritional ingredients. The processes and products illustrated have all been demonstrated to have both highly concentrated functional color properties with acceptable use characteristics (like little flavor or aroma impact), as well as known nutritive qualities, primarily as antioxidants and anti-inflammatory properties.

Accordingly, in one embodiment of the present invention, a natural color is concentrated to intensify color range and to provide useful amounts of one or more of anti-oxidant, nutritional, and anti-inflammatory compounds derived from one or more pigment sources. In a preferred embodiment, the pigment source is a fruit, a vegetable, a legume, a spice, algae, or a combination thereof.

In one embodiment, the pigment or pigments in the color are extracted from or concentrated from one or a combination of grape, beet, red cabbage, red radish, hibiscus, carmine, red sandalwood, purple carrot, black carrot, purple sweet potato, purple corn, black currant, bilberry, elderberry, maqui berry, natural carotenoids, carrot, turmeric, curcumin, paprika, annatto, lutein, marigold, spinach, chlorophyll, and spirulina. In one embodiment, the pigment or pigments in the color are extracted by one of or a combination of screw press, hydraulic press, juicing, natural solvent extraction, synthetic solvent extraction, and distillation.

In a variation of this embodiment, the natural color is further processed by one or a combination of vacuum concentration, steam concentration, supercritical carbon dioxide extraction, distillation, ultra-filtration, membrane filtration, column purification, and ion exchange. In one embodiment, the color compound is dried using one or a combination of spray drying, vacuum drying, drum drying, refractance window drying, radiant zone drying and freeze drying.

In one embodiment, the color compound is endogenous. In another embodiment, the color compound is exogenous. In a variation of the endogenous embodiment, the color compound includes one or more of vitamins, minerals, fats, proteins, and sugars. In a variation of the exogenous embodiment, the color compound includes one or a combination of rosemary, butylated hydroxytoulene (BHT), citrus oils, citric acid, and potassium sorbate. In another variation of the exogenous embodiment, the color compound includes one or more of the compounds co-enzyme Q10 (CoQIO), resveratrol, statins, phytosterols, and dietary fiber.

In yet another variation of the exogenous embodiment, the color compound includes one or more of polysaccharides, methylxanthine, caffeine, theobromine and theophylline. In another variation of this embodiment, the color compound includes one or more of 1-thiamine, 1-arginine, 1-phenylalanine, 1-tryptophan, rhodiola, and rosea. In a further variation to this embodiment, the natural color compound includes one or more of omega 3 fatty acids, docosahexanoic acid (DHA), eicosapentaenoic acid (EPA), phosphatidyl choline, phosphatidyl serine, and gingko biloba.

BRIEF DESCRIPTION OF THE DRAWING FIGURES

Fig. 1 is an organizational chart 100 listing phenolic compounds and their sources.

Fig. 2 is an organizational chart 200 listing vitamins and their sources.

Fig. 3 is an organizational chart 300 listing carotenoid terpenoids and their sources.

Fig. 4 is a collection of organizational charts 400 listing vitamin co-factors and minerals, organosulpher compounds, and hormones and organic antioxidants and their sources.

DETAILED DESCRIPTION The inventors have discovered that natural colors may be enhanced through a variety of extraction and or concentration methods to include useful amounts of one or more of anti-oxidant, nutritional, and anti-inflammatory compounds derived from one or more pigment sources. The present invention will be described in enabling detail using the following examples, which may describe more than one relevant embodiment falling within the scope of the present invention.

Fig. 1 is an organizational chart 100 listing phenolic compounds and their sources. Fig. 2 is an organizational chart 200 listing vitamins and their sources.

Fig. 3 is an organizational chart 300 listing carotenoid terpenoids and their sources.

Fig. 4 is a collection of organizational charts 400 listing vitamin co-factors and minerals, organosulpher compounds, and hormones and organic antioxidants and their sources.

Manufacturing Methods

Referring now to figs. 1-4, the enhanced composition (natural color) can be achieved through a variety of manufacturing and concentrating techniques. Typically speaking, natural colors are extracted from a variety of natural ingredients including (but not limited to) berries, grapes, carrots (orange and black/purple), beets, purple sweet potato, red cabbage, red radish, purple corn, hibiscus, different marine algae

(including spirulina), paprika, marigold, lutein, annatto, tomato, turmeric and spinach. The natural colors can be extracted from whole products, juices or even waste streams. Natural colors are often extracted using techniques like pressing (screw press, hydraulic press and so on), juicing, solvent extraction (natural and synthetic solvents), distillation as well as supercritical carbon dioxide. The enhanced composition goes beyond these processing techniques and includes additional processes, including: Concentration (vacuum, steam), Supercritical C02 (carbon dioxide) extraction and purification, Distillation, Ultra-Filtration, Membrane

Filtration, Column or Purification, Ion Exchange Resins. Similarly, the enhanced composition can be dried and appropriate technologies for drying include: spray drying, vacuum drying, drum drying, refractance window drying, radiant zone drying and freeze drying.

Families of Antioxidant Compounds

Referring now to Figs. 1-4, while almost all natural colors contain antioxidant and/or anti-inflammatory pigments, not all antioxidants are pigments. The discovery focuses on a select range of antioxidant compounds that have color as well as nutritional value. These families of compounds include, but are not limited to:

Please see the Antioxidant Family Charts in Appendix A for additional characterization of antioxidant compounds into families. Appendix A is incorporated entirely in this specification by reference.

Currently available Natural Colors

Single Ingredients:

Grape/Grape Concentrate/Grape Skin

Beet

Red Cabbage

Red Radish

Hibiscus

Carmine

Red Sandalwood

Purple/Black Carrot

Purple Sweet Potato

Purple Corn Black Currant

Bilberry

Elderberry

Maqui Berry

Natural Caretonoids

Carrot

Turmeric/ Curcumin

Paprika

Annatto

Lutein

Marigold

Spinach

Chlorophyll

Spirulina

Combination of Ingredients - Any combination of these ingredients is also acceptable.

Enhanced Natural Colors

Single Ingredients

Grape/Grape Concentrate/Grape Skin

Beet

Red Cabbage

Red Radish

Hibiscus

Carmine

Red Sandalwood

Purple/Black Carrot

Purple Sweet Potato

Purple Corn

Black Currant

Bilberry

Elderberry

Maqui Berry Natural Carotenoids

Carrot

Turmeric/ Curcumin

Annatto

Lutein

Marigold

Spinach

Chlorophyll

Spirulina

Combination of Ingredients - Any combination of these ingredients is also acceptable.

Defining Typical and Enhanced Ranges

In this disclosure there is broad use of two terms: typical range and enhanced range. Below is a definition for both terms:

Typical range(s) - The typical range refers to the measured ranges (like color density, amount of marker or antioxidant compounds, ORAC values, use rates etc.) for natural color ingredients/products that are currently and typically available in the market today. The range reflects the typically available and typically used color ingredients used in the food, dietary supplement and skin-care industries.

Enhanced range(s) - The enhanced range refers to the measured ranges (like color density, amount of marker or antioxidant compounds, ORAC values, use rates etc.) for natural color ingredients/products that have been concentrated or enhanced to increase levels of antioxidant or anti-inflammatory compounds, ORAC values, as well as have enhanced nutritional benefit.

Color Density Measurements - Typical and Enhanced Ranges

Color density reading is the primary means for evaluating colors. There are a variety of different means of testing color density based on different commercial standards and the characteristics of the product. The most common accepted method, especially for water-soluble compounds, is to measure OD (Optical Density) by diluting the sample and reading it through UV-VIS (ultraviolet to visual spectrum of light) in a spectrophotometer. In Appendix B and Appendix C are color readings and methods for both the typical range of color products in the market and the enhanced ranges. Also in Appendix D are attached written procedures for the analysis of color density.

See Functional Color Analysis Spreadsheet. Also attached, please find written procedures for testing color density (Appendix D). Appendices A, B, C, and D are hereby incorporated into this specification by reference.

Marker Compounds - Typical and Enhanced Ranges

As stated earlier, there are numerous compounds and families of compounds that have the dual purpose of natural color and antioxidant/anti-inf ammatory

(nutritional value). There are also a variety of different methods employed to identify and quantify these compounds. Many of these are commonly accepted or published methods. In Appendix B and Appendix C are marker compound values (and ranges) and methods for both the typical range of color products in the market and the enhanced ranges. Also in Appendix D are attached written procedures for the analysis of marker compounds.

See Functional Color Analysis Spreadsheet. Also attached, please find written procedures for testing marker compounds (Appendix D). USDA Database for the Flavonoid Content of Selected Foods:

The link below is to the USDA's website disclosing the contend of Flavonoids in a broad spectrum of foods and which serves as a guideline for the importance of concentrating and enhancing composition so as to enhance the antioxidant properties of the products.

http://www.ars.usda.gov/SP2UserFiles/Place/12354500/Data/ Flav/Flav02- 1 .pdf

ORAC Values - Typical and Enhanced Ranges

There are a few different methods for analyzing ORAC values and a few variations to the most common method (for determination of water soluble antioxidant capacity - hydrophyllic method). In Appendix B and Appendix C are ORAC values (and ranges) and methods for both the typical range of color products in the market and the enhanced ranges. Also in Appendix D are attached written procedures for the analysis of ORAC values.

See Functional Color Analysis Spreadsheet. Also attached, please find written procedures for testing ORAC value (Appendix D).

Antioxidants and the Importance of Bio-Assays like ORAC

Free radicals are highly reactive compounds which cause damage to cellular components such as DNA and cell membranes. Such damage is called "oxidative damage" and is the common pathway in the aging process, inflammation and such diseases as:

• Cancer

• Diabetes

• Arthritis

Cardiovascular Disease

Antioxidants are incredibly important compounds, which effectively "mop up" free radicals produced through metabolism and environmental stresses. ORAC (Oxygen Radical Absorbance Capacity) assay measures the ability of a substance to disarm oxygen free radicals and thereby inhibiting their ability to cause oxidative damage. The ORAC assay compares a sample to Trolox (a non-commercial water- soluble derivative of tocopherol). The results are then reported as μιηοΐεβ Trolox Equivalents (TE)/g.

This method has become synonymous with antioxidant potency in the dietary supplement industry and in food industry. The ORAC assay can provide a much- needed system to compare the antioxidant capacity of various products to the ORAC intake of healthy diet.

Oxygen Radical Absorbance Capacity (ORAC) of Selected Foods - 2007

The link below is to the USDA's website disclosing the most recent ORAC values for a broad spectrum of foods and which serves as a guideline for the importance of concentrating and enhancing composition so as to enhance the ORAC values of the compounds.

http://www.ars.usda.gov/SP2UserFiles/Place/12354500/Data/ ORAC/ORAC07.pdf Claimed Health Benefits from Enhanced Composition

The enhanced composition of the single and combination products has a variety of health related functions and benefits. The benefits and functions are associated to the antioxidant and anti-inflammatory properties of the enhanced color products. These benefits and functions may include, but are not limited to:

Cardiovascular Benefits

Cholesterol and Plaque Reducing Properties

Immune Enhancing Properties

Micro-circulatory Benefits

Cognitive Health Benefits

Mood Enhancing Properties

Eye Health

Anti-Inflammatory Function

Energy and Endurance Functions

Hormonal Balancing

Sexual Function

Combining Pigments to Enhance Color as well as Nutritional Properties

It is common practice in the natural colors industry to combine different ingredients to produce different shades and hues with specific applications. This same concept is applied to the discovery with added benefits in addition to the hues.

The combination of different nutritional pigments may promote synergistic effects.

Anthocyanins, for example, are a fairly large family of compounds, many of which have specific nutritional function, including antioxidant and anti-inflammatory effects. The combination therefore of different nutritional pigments may have health promoting properties. Some examples of these may include, but are not limited to:

Cardiovascular Benefits

Cholesterol and Plaque Reducing Properties

Immune Enhancing Properties

Micro-circulatory Benefits

Cognitive Health Benefits Mood Enhancing Properties

Eye Health

Anti-Inflammatory Function

Energy and Endurance Functions

Hormonal Balancing

Sexual Function

The combination of these pigments can take place in a variety of different ways, but ideally constitutes blending the different sources of the pigments to produce color and health enhancing properties. All of the color sources listed herein can be mixed and blended to further enhance the nutritional benefits of the blend.

The Relationship of Color Pigments with other Endogenous Compounds in the Matrix

Most of the color ingredients contain antioxidant compounds and these compounds have been concentrated or enhanced to produce the nutritional color products. In many of these cases, the antioxidant compounds are part of a matrix of other compounds that comprise the product. The matrix comprises other endogenous compounds like water, carbohydrates (a variety of different sugars), fats, protein, vitamins and minerals. These other endogenous compounds also perform an important role in the performance of the products, either as stabilizing agents (compounds that help protect the other active ingredients in the matrix) or as supporting nutritional compounds. This is certainly the case of vitamins, minerals, some fats and proteins. It is also true that many of the sugars, especially the complex sugars in some of these products can act as transport compounds. This means these compounds can enhance the delivery of the antioxidant compounds into the blood stream and as a result improve bioavailability as well as enhance the properties of these nutritional colors.

The Relationship of Color Pigments with other Exogenous Compounds in the Matrix

The discovery also recognizes the importance of exogenous, or added, ingredients to the product matrix. There are primarily two types of Exogenous Compounds that go into the matrix: those added to the product to aid in stability or other color function, and those added to the matrix to enhance nutritional properties. This discovery claims that there are many exogenous compounds that fit these purposes and that these compounds may also enhance, preserve, or otherwise support the nutritional value of the pigments.

Exogenous Compounds that aid Stability or other Color Function, including but not limited to:

Rosemary

BHT

Citrus Oils

Citric Acid

Potassium Sorbate

The discovery also claims that exogenous compounds can be added to the matrix that enhance antioxidant, anti-inflammatory or other nutritional qualities to the matrix.

Exogenous Compounds that Enhance Nutritional Properties include, but are not limited to:

All the antioxidants listed in this disclosure, especially under the section "Family of Compounds"

Heart and Cardiovascular health promoting compounds including, but not limited to: Co-enzyme Q10 (CoQlO), Resveratrol, statins, phytosterols, dietary fiber, polysaccharides

Energy compounds including, but not limited to: methylxanthine alkaloids (caffeine, theobromine and theophylline) along with their natural sources (coffee, tea, green tea, white tea, mate, cocoa, kola nut)

Mood enhancing compounds including, but not limited to: L-theanine, amino acids (especially L-arginine, L-Phenylalanine, L-Tryptophan), Rhodiola rosea, Cognitive enhancing compounds including, but not limited to: Omega 3 Fatty Acids (from Fish and Vegetable oils), DHA & EPA, Phosphatidyl Choline, Phosphatidyl Serine, Gingko Biloba It will be apparent to one with skill in the art that the natural color of the invention may be provided using some or all of the mentioned features and components without departing from the spirit and scope of the present invention. It will also be apparent to the skilled artisan that the embodiments described above are specific examples of a single broader invention that may have greater scope than any of the singular descriptions taught. There may be many alterations made in the descriptions without departing from the spirit and scope of the present invention.

Appendix D

Attachment

Oxygen Radical Absorbance Capacity (ORAC) of Selected Foods - 2007

Prepared by

Nutrient Data Laboratory

Beltsville Human Nutrition Research Center (BHNRC)

Agricultural Research Service (ARS)

U.S. Department of Agriculture (USDA) in collaboration with

Arkansas Children's Nutrition Center, ARS, USDA, Little Rock, AR

November 2007

U.S. Department of Agriculture

Agricultural Research Service

Beltsville Human Nutrition Research Center

Nutrient Data Laboratory

10300 Baltimore Avenue

Building 005, Room 107, BARC-West

Beltsville, Maryland 20705

Tel. 301-504-0630, FAX: 301-504-0632

E-Mail: ndlinfo@.ars.usda.gov

Web site: http://www.ars.usda.gov/nutrientdata Table of Contents

Introduction 1

Methods 2

Data Evaluation 2

File Formats 3

References used in the documentation 4

Oxygen Radical Absorbance Capacity (ORAC) of Selected Foods 6

Sources of Data 33

Oxygen Radical Absorbance Capacity (ORAC) of Selected Foods - 2007 Introduction

The development of many chronic and degenerative diseases, such as cancer (1), heart disease (5), and neuronal degeneration such as Alzheimer's (4) and Parkinson's disease (9) has been theorized to be caused, in part, by oxidative stress. Oxidative stress has also been implicated in the process of aging (2). It is known that reactive oxygen species can damage biological molecules such as proteins, lipids, and DNA. While the human body has developed a number of systems to eliminate free radicals from the body, it is not 100% efficient (20).

Diets rich in fruits, nuts, and vegetables have long been considered to be an excellent source of antioxidants. A number of minerals and vitamins have a role as dietary antioxidants in addition to their other biological functions. These include vitamin C (ascorbic acid), vitamin E and its isomers (tocopherols and tocotrienols), and selenium. Data for these are included in the USDA National Nutrient Database for Standard Reference (SR) (18). USDA has also published a number of Special Interest Databases on various antioxidants: Carotenoids (14) (now merged with SR); isoflavones (15), flavonoids (16), and proanthocyanidins (17). However, there is no database of antioxidant activity for selected foods.

As part of the National Food and Nutrient Analysis Program (NFNAP) (12), USDA, in collaboration with the Produce for Better Health Foundation, undertook the analysis of 59 individual fruits, nuts, and vegetables. In addition to the traditional proximates, minerals, and vitamins analyses, which are included in SR, these foods were analyzed for a number of potentially bioactive compounds. These foods, along with a few foods collected for the food composition database for American Indians and Alaskan Natives, were also analyzed for their oxygen radical absorbance capacity (ORAC) by Wu et al. (19) at the Arkansas Children's Nutrition Center, ARS, USDA. These data as well as a limited amount of analytical data from the literature were compiled for the ORAC database.

In addition to the ORAC assay, other common measures of antioxidant capacity (AC) include ferric ion reducing antioxidant power (FRAP) and trolox equivalence antioxidant capacity (TEAC) assays. These assays are based on different underlying mechanisms using different radical or oxidant sources and therefore generate different values and cannot be compared directly. The ORAC assay is considered by some to be a preferable method because of its biological relevance to the in vivo antioxidant efficacy (3). In general, a food that has a high value for one measure of AC will also be high for another measure. However, because antioxidant compounds with different chemical structures interact with different radical sources differently, the relationship between any two AC methods will be quite low if considered across all foods. Thus, it is not possible to develop a mathematical relationship between 2 methods across a wide spectrum of foods. Like the content of any food component, AC values will differ due to a wide array of reasons, such as cultivar, growing conditions, harvesting, food processing and

preparation, sampling, and analytical procedures. Methods

The analytical method developed by Prior et al (13) was used as the reference method for evaluating analytical methods from other published sources. This method uses fluorescein as the fluorescent probe and assays hydrophilic as well as lipophilic antioxidants. Analytical data from literature based on methods that used B-phycoerythrin (B-PE) as the probe were not used in this compilation as B-PE may produce inconsistent results in some foods, is not photostable, and may involve nonspecific protein binding with polyphenols (11).

ORAC Values are reported for hydrophilic-ORAC (H-ORAC), lipophilic-ORAC (L- ORAC), total-ORAC, and total phenolics (TP). H-ORAC, L-ORAC and total-ORAC are reported in μmol of Trolox Equivalents per 100 grams ( μmolΤΕ/100 g), while TP is reported in mg gallic acid equivalents per 100 grams (mgGAE/100 g). When only an H- ORAC value was available for a particular food item low in fat, H-ORAC value was also utilized for the Total ORAC value. In some cases values for H-ORAC, L-ORAC and Total-ORAC may come from different sources, and the sum of the average values for H- ORAC and L-ORAC may not equal the value for Total-ORAC.

Data Evaluation

The data were evaluated for quality using procedures developed by scientists at the NDL as part of the Nutrient Databank System (7). These procedures were based on criteria described earlier (6, 10) with some modifications. Procedures developed for the first release in 2003 of the flavonoid database were followed (8). The five categories of documentation which were evaluated included: sampling plan, sample handling, number of samples, analytical method, and analytical quality control. NDL modified the criteria for the sampling plan rating at the aggregation stage to accommodate data from international sources. For aggregated data which included data from countries other than the United States, the number of countries replaced the number of regions within a country. The information presented in each reviewed paper was evaluated for each category, which then received a rating ranging from 0 to 20 points. The ratings for each of the five categories are summed to yield a quality index (QI) with the maximum possible score of 100 points. A confidence code (CC) is derived from the QI and is an indicator of the relative quality of the data and the reliability of a given mean. The CC is assigned as indicated in Table 1 :

The data were aggregated where possible to match the food descriptions in the USDA National Nutrient Database for Standard Reference (SR). Subsequently, the mean value (mg/lOOg), standard error of the mean (SEM), minimum (Min), and maximum (Max.) values were determined for each food and ORAC value. Mean values are weighted to account for the different number of samples among the various studies used. The weighted mean is, in turn, used to calculate the standard error based on the total number of samples in each aggregated food.

File Formats

Each food was given a Nutrient Data Bank (NDB) number, the five digit numerical code used in the SR to identify each unique food entry if it matches a food in SR. As the data came from various sources both in the United States and other countries, there are a number of foods which are not included in the SR database. Temporary NDB numbers, beginning with either "97" or "99", were assigned to foods that are not included in the SR. While efforts were made to assign the same "temporary" NDB Numbers to the same foods as in other Special Interest Databases, some numbers may have been used to encode different foods in other Special Interest Databases produced by the NDL, and therefore may not be unique. Minimum and maximum values are not reported when the number of samples = 1. A reference number corresponding to the publications in the sources of data section of the documentation is included in the table. Table 2 contains ORAC values for 277 foods and is arranged in alphabetical order and is also provided as a Microsoft Access database.

This table of ORAC values will provide the user with a listing of antioxidant capacity as measured by the oxygen radical absorbance capacity method for a number of food items. When used in tandem with the phytonutrient Special Interest Tables developed by NDL, the user can assess the various sources of antioxidants in the food supply.

References used in the documentation

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2. Ames, B. N., Shigenaga, M. K. & Hagen, T. M. (1993) Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA. 90: 7915- 7922.

3. Awika, J. M., Rooney, L. W., Wu, X., Prior, R. L., and Cisneros-Zevallos, L.

Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J. Agric. Food Chem.,2003, 51 :6657-6662.

4. Christen, Y. (2000) Oxidative stress and Alzheimer disease. Am. J. Clin. Nutr.

71 : 621S-629S.

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atherosclerotic heart disease. New Eng. J. Med. 337: 408-416.

6. Holden, J. M., Schubert, A., Wolf, W. R., Beecher, G. R. 1987. A system for evaluating the quality of published nutrient data: Selenium, a test case. Food Nutr. Bull. 9(suppl), 177-193.

7. Holden, J. M., Bhagwat, S. A., Patterson, K. Y. 2002. Development of a multi- nutrient data quality evaluation system. J. Food Comp. Anal. 15, 339-348.

8. Holden, J.M., Bhagwat, S.A., Beecher, G.R., Haytowitz, D.B., Gebhardt, S.E., Eldridge, A.L., Dwyer, J., and Peterson, J. 2005. Development of a Database of Critically Evaluated Flavonoids Data: Application of USDA's Data Quality Evaluation. J. Food Comp. Anal. 18:829-844.

9. Lang, A. E. & Lozano, A. M. (1998) Parkinson's disease. First of two parts.

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