Title:
ENHANCED PROTEIN EXPRESSION
Document Type and Number:
WIPO Patent Application WO/2011/085095
Kind Code:
A3
Abstract:
The application describes methods to enhance protein production using mammalian cells. Increased production of heterologous protein can be obtained by increasing osmolality and lowering temperature. The method maintains the cell growth rate and provides high product yield.
Inventors:
SATAKARNI MAKKAPATI (IN)
VAIBHAV S NIKAM (IN)
SATYAM SUBRAHMANYAM (IN)
VAIBHAV S NIKAM (IN)
SATYAM SUBRAHMANYAM (IN)
Application Number:
PCT/US2011/020367
Publication Date:
November 17, 2011
Filing Date:
January 06, 2011
Export Citation:
Assignee:
REDDYS LAB LTD DR (IN)
REDDYS LAB INC DR (US)
SATAKARNI MAKKAPATI (IN)
VAIBHAV S NIKAM (IN)
SATYAM SUBRAHMANYAM (IN)
REDDYS LAB INC DR (US)
SATAKARNI MAKKAPATI (IN)
VAIBHAV S NIKAM (IN)
SATYAM SUBRAHMANYAM (IN)
International Classes:
C12N15/63; C12N5/02; C12P21/08
Other References:
KIM ET AL.: "Biphasic culture strategy based on hyperosmotic pressure for improved humanized antibody production in Chinese hamster ovary cell culture", IN VITRO CELL DEV BIOL ANIM., vol. 38, no. 6, June 2002 (2002-06-01), pages 314 - 319, XP008160821
WULHFARD ET AL.: "Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells", BIOTECHNOL PROG., vol. 24, no. 2, 26 January 2008 (2008-01-26), pages 458 - 465, XP008160823
RYU ET AL.: "Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant chinese hamster ovary cell cultures differs among cell lines", BIOTECHNOL BIOENG., vol. 70, no. 2, 20 October 2000 (2000-10-20), pages 167 - 175, XP008160825
MARCHANT ET AL.: "Metabolic rates, growth phase, and mRNA levels influence cell-specific antibody production levels from in vitro-cultured mammalian cells at sub-physiological temperatures", MOL BIOTECHNOL., vol. 39, no. 1, 6 February 2008 (2008-02-06), pages 69 - 77, XP008160826
FAN ET AL.: "A high-yielding, generic fed-batch process for recombinant antibody production of GS-engineered cell lines", J MICROBIOL BIOTECHNOL., vol. 19, no. 12, December 2009 (2009-12-01), pages 1695 - 1702, XP008160830
DEZENGOTITA ET AL.: "Characterization of hybridoma cell responses to elevated pCO(2) and osmolality: intracellular pH, cell size, apoptosis, and metabolism", BIOTECHNOL BIOENG., vol. 77, no. 4, 15 February 2002 (2002-02-15), pages 369 - 380, XP055016503
WULHFARD ET AL.: "Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells", BIOTECHNOL PROG., vol. 24, no. 2, 26 January 2008 (2008-01-26), pages 458 - 465, XP008160823
RYU ET AL.: "Osmoprotective effect of glycine betaine on foreign protein production in hyperosmotic recombinant chinese hamster ovary cell cultures differs among cell lines", BIOTECHNOL BIOENG., vol. 70, no. 2, 20 October 2000 (2000-10-20), pages 167 - 175, XP008160825
MARCHANT ET AL.: "Metabolic rates, growth phase, and mRNA levels influence cell-specific antibody production levels from in vitro-cultured mammalian cells at sub-physiological temperatures", MOL BIOTECHNOL., vol. 39, no. 1, 6 February 2008 (2008-02-06), pages 69 - 77, XP008160826
FAN ET AL.: "A high-yielding, generic fed-batch process for recombinant antibody production of GS-engineered cell lines", J MICROBIOL BIOTECHNOL., vol. 19, no. 12, December 2009 (2009-12-01), pages 1695 - 1702, XP008160830
DEZENGOTITA ET AL.: "Characterization of hybridoma cell responses to elevated pCO(2) and osmolality: intracellular pH, cell size, apoptosis, and metabolism", BIOTECHNOL BIOENG., vol. 77, no. 4, 15 February 2002 (2002-02-15), pages 369 - 380, XP055016503
Attorney, Agent or Firm:
FRANKS, Robert A. (Inc.200 Somerset Corporate Boulevard 7th Floo, Bridgewater New Jersey, US)
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