Title:
ENHANCING SPECIFICITY OF ANALYTE BINDING
Document Type and Number:
WIPO Patent Application WO/2021/092433
Kind Code:
A3
Abstract:
Methods for enhancing specificity of an analyte binding moiety or probe oligonucleotide to an analyte are provided herein. For example, methods provided herein include blocking a capture binding domain, thereby preventing hybridization to the capture domain of the capture probe affixed to a substrate. Further methods include releasing the block from the capture binding domain, thereby allowing the capture binding domain to specifically bind to the capture domain of the capture probe on the substrate.
Inventors:
STOECKIUS MARLON (US)
Application Number:
PCT/US2020/059472
Publication Date:
July 01, 2021
Filing Date:
November 06, 2020
Export Citation:
Assignee:
10X GENOMICS INC (US)
STOECKIUS MARLON (US)
STOECKIUS MARLON (US)
International Classes:
C12Q1/6874
Domestic Patent References:
| WO2016168825A1 | 2016-10-20 | |||
| WO2006124771A2 | 2006-11-23 | |||
| WO2012049316A1 | 2012-04-19 | |||
| WO2018075436A1 | 2018-04-26 | |||
| WO2011127099A1 | 2011-10-13 |
Foreign References:
| US20070161029A1 | 2007-07-12 | |||
| US20150148239A1 | 2015-05-28 |
Other References:
JOEL J. CREDLE ET AL: "Multiplexed analysis of fixed tissue RNA using Ligation in situ Hybridization", NUCLEIC ACIDS RESEARCH, vol. 45, no. 14, 19 June 2017 (2017-06-19), pages e128 - e128, XP055525254, ISSN: 0305-1048, DOI: 10.1093/nar/gkx471
J. H. LEE ET AL: "Highly Multiplexed Subcellular RNA Sequencing in Situ", SCIENCE, vol. 343, no. 6177, 27 February 2014 (2014-02-27), US, pages 1360 - 1363, XP055305772, ISSN: 0036-8075, DOI: 10.1126/science.1250212
K. H. CHEN ET AL: "Spatially resolved, highly multiplexed RNA profiling in single cells", SCIENCE, vol. 348, no. 6233, 9 April 2015 (2015-04-09), US, pages aaa6090 - aaa6090, XP055391215, ISSN: 0036-8075, DOI: 10.1126/science.aaa6090
STÅHL PATRIK L ET AL: "Visualization and analysis of gene expression in tissue sections by spatial transcriptomics", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 353, no. 6294, 1 July 2016 (2016-07-01), pages 78 - 82, XP002784680, ISSN: 1095-9203, DOI: 10.1126/SCIENCE.AAF2403
J. H. LEE ET AL: "Highly Multiplexed Subcellular RNA Sequencing in Situ", SCIENCE, vol. 343, no. 6177, 27 February 2014 (2014-02-27), US, pages 1360 - 1363, XP055305772, ISSN: 0036-8075, DOI: 10.1126/science.1250212
K. H. CHEN ET AL: "Spatially resolved, highly multiplexed RNA profiling in single cells", SCIENCE, vol. 348, no. 6233, 9 April 2015 (2015-04-09), US, pages aaa6090 - aaa6090, XP055391215, ISSN: 0036-8075, DOI: 10.1126/science.aaa6090
STÅHL PATRIK L ET AL: "Visualization and analysis of gene expression in tissue sections by spatial transcriptomics", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 353, no. 6294, 1 July 2016 (2016-07-01), pages 78 - 82, XP002784680, ISSN: 1095-9203, DOI: 10.1126/SCIENCE.AAF2403
Attorney, Agent or Firm:
KNABEL, Matthew K. et al. (US)
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