Title:
ENZYMATIC METHOD FOR INDUSTRIAL PRODUCTION OF NAD
Document Type and Number:
WIPO Patent Application WO/2020/258111
Kind Code:
A1
Abstract:
An enzymatic method for the industrial production of NAD. The method uses nicotinamide ribose (NR) and ATP as raw materials and conducts a biocatalytic reaction in the presence of NAD synthetase to produce NAD, wherein, the NAD synthetase comprises a nicotinamide mononucleotide adenosine transferase structure domain derived from Haemophilus influenzae as well as a nicotinamide ribokinase structure domain derived from any among humans, Saccharomyces cerevisiae, Escherichia coli, and Salmonella typhimurium. The method may achieve one-step catalytic production of NAD using NR and ATP as raw materials.
Inventors:
ZHANG ZHANG (CN)
ZHANG QI (CN)
ZHANG QI (CN)
Application Number:
PCT/CN2019/093140
Publication Date:
December 30, 2020
Filing Date:
June 27, 2019
Export Citation:
Assignee:
BONTAC INVITROLIFE BIO TECH SHENZHEN CO LTD (CN)
BONTAC BIO ENG SHENZHEN CO LTD (CN)
BONTAC BIO ENG SHENZHEN CO LTD (CN)
International Classes:
C12P19/32
Foreign References:
| CN108998484A | 2018-12-14 | |||
| CN102471793A | 2012-05-23 | |||
| CN107955791A | 2018-04-24 | |||
| CN106884028A | 2017-06-23 |
Other References:
SINGH S. KUMAR, KURNASOV OLEG V., CHEN BAOZHI, ROBINSON HOWARD, GRISHIN NICK V., OSTERMAN ANDREI L., ZHANG HONG: "Crystal Structure of Haemophilus influenzae NadR Protein", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 36, 14 June 2002 (2002-06-14), American Society for Biochemistry and Molecular Biology, pages 33291 - 33299, XP055774555, ISSN: 0021-9258, DOI: 10.1074/jbc.M204368200
KURNASOV, O.V. ET AL.: "Ribosylnicotinamide Kinase Domain of NadR Protein: Identification and Implications in NAD Biosynthesis", JOURNAL OF BACTERIOLOGY, vol. 184, no. 24, 31 December 2002 (2002-12-31), XP055770659, DOI: 20200306151143
KURNASOV, O.V. ET AL.: "Ribosylnicotinamide Kinase Domain of NadR Protein: Identification and Implications in NAD Biosynthesis", JOURNAL OF BACTERIOLOGY, vol. 184, no. 24, 31 December 2002 (2002-12-31), XP055770659, DOI: 20200306151143
Download PDF:
Previous Patent: RECOMBINANT NAD SYNTHETASE AND GENE AND USE THEREOF
Next Patent: NOVEL CAMBERED SURFACE TYPE ELECTRIC SHAVER
Next Patent: NOVEL CAMBERED SURFACE TYPE ELECTRIC SHAVER