Title:
ENZYMATIC PREPARATION METHOD FOR OXIDIZED COENZYME II
Document Type and Number:
WIPO Patent Application WO/2014/146242
Kind Code:
A1
Abstract:
The present invention relates an enzymatic preparation method for an oxidized coenzyme II using a one pot method. Three substrates: nicotinamide nucleotide (NR), adenosine triphosphate disodium salt (ATP-Na2), and sodium hexametaphosphate are used as raw materials, to make them react in the existence of four enzymes: nicotinamide nucleoside kinase (NRK), inorganic pyrophosphatase, NAD kinase, and poly-pyrophosphokinase, in a buffer solution with pH being 4.0 to 8.5, and at a temperature of 10°C to 40°C, so as to obtain the oxidized coenzyme II. The present invention solves the technical problem that nicotinamide nucleotide has a high cost and is difficult to be obtained, the reaction time is long, a process cost is high, and a process condition is not suitable for industrial scale-up in a current enzymatic preparation method; and the oxidized coenzyme II can be obtained with high efficiency and a low cost in an industrialized scale-up production manner.
Inventors:
TAO JUNHUA (CN)
LI BIN (CN)
XIE LEI (CN)
ZHUANG JICHANG (CN)
ZHOU YONGDA (CN)
ZHANG CHAO (CN)
LIU GEN (CN)
LI BIN (CN)
XIE LEI (CN)
ZHUANG JICHANG (CN)
ZHOU YONGDA (CN)
ZHANG CHAO (CN)
LIU GEN (CN)
Application Number:
PCT/CN2013/072845
Publication Date:
September 25, 2014
Filing Date:
March 19, 2013
Export Citation:
Assignee:
ENZYMEWORKS INC (CN)
International Classes:
C12P19/36
Foreign References:
| CN102605027A | 2012-07-25 | |||
| EP1416051A1 | 2004-05-06 | |||
| CN102605026A | 2012-07-25 | |||
| US4411995A | 1983-10-25 |
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT & TRADEMARK AGENCY LTD. (CN)
苏州创元专利商标事务所有限公司 (CN)
苏州创元专利商标事务所有限公司 (CN)
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