Title:
ENZYME CO-EXPRESSION SYSTEM AND USE THEREOF IN SYNTHESIS OF SIALIC ACID
Document Type and Number:
WIPO Patent Application WO/2023/184822
Kind Code:
A1
Abstract:
Provided are an N-acetylneuraminic acid aldolase (NAL) mutant, a system for co-expressing same and N-acetylglucosamine-2-epimerase (AGE), and use thereof in the synthesis of sialic acid. By adopting a co-expression system of dual promoters, the simultaneous over-expression of two enzymes is realized, and the reaction efficiency of an enzyme-catalyzed reaction is improved. At the same time, the mutation modification in NAL improves the synthesis efficiency of sialic acid produced by a forward reaction.
Inventors:
ZHAO HONG (CN)
QIN GUOFU (CN)
YU TIEMEI (CN)
PAN JUNFENG (CN)
LIU JIAN (CN)
QIN GUOFU (CN)
YU TIEMEI (CN)
PAN JUNFENG (CN)
LIU JIAN (CN)
Application Number:
PCT/CN2022/112444
Publication Date:
October 05, 2023
Filing Date:
August 15, 2022
Export Citation:
Assignee:
SHENZHEN READLINE BIOTECH CO LTD (CN)
International Classes:
C12N9/88; A23L33/00; A61K8/60; A61Q19/00; C12N1/21; C12N15/60
Foreign References:
| CN114507658B | 2022-07-05 | |||
| CN102649965A | 2012-08-29 | |||
| CN113337495A | 2021-09-03 | |||
| CN104001165A | 2014-08-27 | |||
| CN101668850A | 2010-03-10 | |||
| AU2012261528A1 | 2013-01-10 |
Other References:
DATABASE Protein 28 July 2021 (2021-07-28), ANONYMOUS : "dihydrodipicolinate synthase family protein [[Clostridium] scindens]", XP093097730, retrieved from NCBI Database accession no. WP_004605209
DATABASE Protein 21 July 2021 (2021-07-21), ANONYMOUS : "AGE family epimerase/isomerase [Bacteroides fragilis] ", XP093097735, retrieved from NCBI Database accession no. WP_010992631
XU YANG, CHEN FANG, LIN BAI-XUE, TAO YONG: "Optimization of Whole-Cell Biocatalytic Process for N-Acetylneuraminic Acid Production", MICROBIOLOGY CHINA, INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES; CHINESE MICROBIOLOGY, CN, vol. 40, no. 8, 31 August 2013 (2013-08-31), CN , pages 1331 - 1338, XP009550235, ISSN: 0253-2654, DOI: 10.13344/j.microbiol.china.2013.08.006
DATABASE Protein 21 July 2021 (2021-07-21), ANONYMOUS : "AGE family epimerase/isomerase [Bacteroides fragilis] ", XP093097735, retrieved from NCBI Database accession no. WP_010992631
XU YANG, CHEN FANG, LIN BAI-XUE, TAO YONG: "Optimization of Whole-Cell Biocatalytic Process for N-Acetylneuraminic Acid Production", MICROBIOLOGY CHINA, INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES; CHINESE MICROBIOLOGY, CN, vol. 40, no. 8, 31 August 2013 (2013-08-31), CN , pages 1331 - 1338, XP009550235, ISSN: 0253-2654, DOI: 10.13344/j.microbiol.china.2013.08.006
Attorney, Agent or Firm:
SHENPAT INTELLECTUAL PROPERTY AGENCY (CN)
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