Title:
ENZYME COMPLEX AND SELF-ASSEMBLY CATALYTIC NANOWIRES
Document Type and Number:
WIPO Patent Application WO/2019/091454
Kind Code:
A1
Abstract:
Provided is the use of a fibroblast protein in enhancing enzyme catalytic activity and/or enzyme stability. The present invention displays enzyme molecules with high density and arrayed on the surface of the nanostructure of the fibroblast protein by the self-assembly of the structure of a fibrillar protein to form a linear nanostructure having catalytic capacity. The nanostructure simulates the natural regionalization status of intracellular enzyme molecules, thereby improves the enzyme catalytic activity and stability without modifying the enzyme molecules themselves.
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Inventors:
MEN DONG (CN)
ZHANG XIAN'EN (CN)
WEI CUIHUA (CN)
ZHANG XIAN'EN (CN)
WEI CUIHUA (CN)
Application Number:
PCT/CN2018/114833
Publication Date:
May 16, 2019
Filing Date:
November 09, 2018
Export Citation:
Assignee:
WUHAN INST VIROLOGY CAS (CN)
International Classes:
C12N9/96
Other References:
MEN, DONG ET AL.: "Self-Assembled Virus-Like Nanostructure and Ultra-Sensitive Biosensing", JOURNAL OF SOUTHEAST UNIVERSITY (MEDICAL SCIENCE EDITION, vol. 30, no. 1, 28 February 2011 (2011-02-28), ISSN: 1671-6264
LENG, YAN ET AL.: "Integration of a Fluorescent Molecular Biosensor into Self-Assembled Protein Nanowires: a Large Sensitivity Enhancement", ANGEW CHEM INT ED ENGL., vol. 49, no. 40, 24 September 2010 (2010-09-24), XP055444775, ISSN: 1521-3773, DOI: doi:10.1002/anie.201002452
MEN, DONG ET AL.: "Seeding-Induced Self-Assembling Protein Nanowires Dramatically Increase the Sensitivity of Immunoassays", NANO LETT., vol. 9, no. 6, 30 June 2009 (2009-06-30), XP055444773, ISSN: 1530-6984, DOI: doi:10.1021/nl9003464
LENG, YAN: "High Enzyme Activity Screening of Methyl Parathion Hydrolase and Study of Nanowire Fluorescent Molecular Biosensor", CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, BASIC SCIENCES, 15 April 2011 (2011-04-15), ISSN: 1674-022X
YIN, WEN ET AL.: "Sup35 (Mechanism and Application of Molecular Self-Assembly in Sup35 Prion Domain of Saccharomyces Cerevisiae", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 27, 25 October 2011 (2011-10-25), XP055607872, ISSN: 1000-3061
LENG, YAN ET AL.: "Integration of a Fluorescent Molecular Biosensor into Self-Assembled Protein Nanowires: a Large Sensitivity Enhancement", ANGEW CHEM INT ED ENGL., vol. 49, no. 40, 24 September 2010 (2010-09-24), XP055444775, ISSN: 1521-3773, DOI: doi:10.1002/anie.201002452
MEN, DONG ET AL.: "Seeding-Induced Self-Assembling Protein Nanowires Dramatically Increase the Sensitivity of Immunoassays", NANO LETT., vol. 9, no. 6, 30 June 2009 (2009-06-30), XP055444773, ISSN: 1530-6984, DOI: doi:10.1021/nl9003464
LENG, YAN: "High Enzyme Activity Screening of Methyl Parathion Hydrolase and Study of Nanowire Fluorescent Molecular Biosensor", CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, BASIC SCIENCES, 15 April 2011 (2011-04-15), ISSN: 1674-022X
YIN, WEN ET AL.: "Sup35 (Mechanism and Application of Molecular Self-Assembly in Sup35 Prion Domain of Saccharomyces Cerevisiae", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 27, 25 October 2011 (2011-10-25), XP055607872, ISSN: 1000-3061
Attorney, Agent or Firm:
BEIJING BRIGHT IP AGENCY CO., LTD. (CN)
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