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Title:
ENZYME DERIVED FROM P AENIBACILLUS PABULI AND CAPABLE OF PRODUCING GALACTOOLIGOSACCHARIDE AND METHOD FOR PRODUCING GALACTOOLIGOSACCHARIDE
Document Type and Number:
WIPO Patent Application WO/2019/194062
Kind Code:
A1
Abstract:
[Problem] To provide: a novel enzyme which is derived from a microorganism other than Bacillus circulans and is highly selective for galactotrisaccharide production and with which the galactooligosaccharide can be highly efficiently produced; and a novel method capable of producing the galactooligosaccharide. [Solution] A method for producing a galatooligosaccharide, the method including bringing an enzyme having an amino acid sequence selected from the group consisting of (a) to (f) and cells of a bacterium belonging to the genus Paenibacillus and/or a galactooligosaccharide-producing enzyme for the bacterium, into contact with lactose. (a) An amino acid sequence of sequence number 1. (b) An amino acid sequence of sequence number 2. (c) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence being the amino acid sequence of sequence number 1 in which one to ten amino acids have been replaced, removed, or inserted. (d) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence being the amino acid sequence of sequence number 2 in which one to ten amino acids have been replaced, removed, or inserted. (e) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence having a homology of 80% or higher but less than 100% to the amino acid sequence of sequence number 1. (f) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence having a homology of 80% or higher but less than 100% to the amino acid sequence of sequence number 2.

Inventors:
ODAKA, Rei (Enzyme & Pharmaceuticals Research Laboratory, 250 Aza-nakahara, Kamihongo, Matsudo-sh, Chiba 64, 〒2710064, JP)
BABA, Yasuhiro (Enzyme & Pharmaceuticals Research Laboratory, 250 Aza-nakahara, Kamihongo, Matsudo-sh, Chiba 64, 〒2710064, JP)
OGASAWARA, Junki (Enzyme & Pharmaceuticals Research Laboratory, 250 Aza-nakahara, Kamihongo, Matsudo-sh, Chiba 64, 〒2710064, JP)
YOSHIKAWA, Jun (Enzyme & Pharmaceuticals Research Laboratory, 250 Aza-nakahara, Kamihongo, Matsudo-sh, Chiba 64, 〒2710064, JP)
Application Number:
JP2019/013549
Publication Date:
October 10, 2019
Filing Date:
March 28, 2019
Export Citation:
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Assignee:
GODO SHUSEI CO., LTD. (6-2-10 Ginza, Chuo-ku Tokyo, 61, 〒1040061, JP)
International Classes:
C07K14/195; C07H3/06; C12N1/13; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/56; C12P19/04
Other References:
LIU, YU ET AL.: "Biochemical characterization of a novel beta -galactosidase from Paenibacillus barengoltzii suitable for lactose hydrolysis and galactooligosaccharides synthesis", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 104, 2017, pages 1055 - 1063
BENESOVA, EVA ET AL.: "beta -D-Galactosidase from Paenibacillus thiaminolyticus catalyzing transfucosylation reactions", GLYCOBIOLOGY, vol. 20, 2010, pages 442 - 451, XP055645233
LEE, JAE-CHAN ET AL.: "Paenibacillus woosongensis sp. nov. , a xylanolytic bacterium isolated from forest soil", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 58, 2008, pages 612 - 616, XP055429335
RIVAS, RAUL ET AL.: "Paenibacillus xylanilyticus sp. nov. , an airborne xylanolytic bacterium", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 55, 2005, pages 405 - 408, XP055645243
IVY, REID A. ET AL.: "Identification and Characterization of Psychrotolerant Sporeformers Associated with Fluid Milk Production and Processing", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 78, 2012, pages 1853 - 1864, XP055645260
ISORNA, PABLO ET AL.: "Crystal Structures of Paenibacillus polymyxa beta -Glucosidase B Complexes Reveal the Molecular Basis of Substrate Specificity and Give New Insights into the Catalytic Machinery of Family I Glycosidases", J. MOL. BIOL., vol. 371, 2007, pages 1204 - 1218, XP022189922
SUMIDA, TOMOMI ET AL.: "Utilization of Ganglioside-Degrading Paenibacillus sp. Strain TS12 for Production of Glucosylceramide", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, 2002, pages 5241 - 5248, XP055645271
Attorney, Agent or Firm:
ITOH, Atsushi (Togeki Building 4F, 4-1-1 Tsukiji Chuo-k, Tokyo 45, 〒1040045, JP)
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