ENZYME IMMOBILIZATION CARRIER AND PREPATION METHOD THEREFOR, AND IMMOBILIZED ENZYME AND PREPARATION METHOD THEREFOR

Title:

ENZYME IMMOBILIZATION CARRIER AND PREPATION METHOD THEREFOR, AND IMMOBILIZED ENZYME AND PREPARATION METHOD THEREFOR

Document Type and Number:

WIPO Patent Application WO/2023/087270

Kind Code:

A1

Abstract:

Provided are an enzyme immobilization carrier and a preparation method therefor, and an immobilized enzyme and a preparation method therefor. The enzyme immobilization carrier comprises a resin sphere matrix, an -N(CH2COOH)2 group linked to the resin sphere matrix by means of a chemical bond, and a metal ion adsorbed on the -N(CH2COOH)2 group by means of a coordination action, wherein the resin sphere matrix is an amino-type methacrylic resin and/or epoxy-type methacrylic resin, and the resin sphere matrix has a particle size of 200-700 μm. The problems in the prior art that an enzyme immobilization carrier is relatively poor in enzyme compatibility and poor in immobilization effect, and an immobilized enzyme is relatively poor in activity, and relatively poor in stability during repeated use are solved.

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Inventors:

HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
VYASA WILLIAMS (US)
ZHAO JIADONG (CN)
PAN LONG (CN)
MA LITENG (CN)
CUI YUXIA (CN)

Application Number:

PCT/CN2021/131878

Publication Date:

May 25, 2023

Filing Date:

November 19, 2021

Export Citation:

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Assignee:

LIAONING ASYMCHEM LABORATORIES CO LTD (CN)

International Classes:

C12N11/087; C08F8/00; C08F20/10; C12N9/02; C12N9/04; C12N9/06; C12N9/10

Foreign References:

CN108384767A	2018-08-10
CN111117995A	2020-05-08
CN105219745A	2016-01-06
US20210054428A1	2021-02-25
JP2001029783A	2001-02-06

Other References:

CHEN HAIXIN; ZHANG SAINAN; ZHAO LIMIN; CHEN YAO: "Enzyme Immobilization: from Strategies to Materials Design", CHINESE JOURNAL OF BIOPROCESS ENGINEERING, vol. 18, no. 1, 15 January 2020 (2020-01-15), pages 88 - 95, XP009546480, ISSN: 1672-3678, DOI: 10.3969/j.issn.1672-3678.2020.01.011

Attorney, Agent or Firm:

KANGXIN PARTNERS, P.C. (CN)

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