Title:
ENZYME MEASUREMENT METHOD, MICROCHAMBER ARRAY, KIT, METHOD FOR CAPTURING RAMAN SCATTERING IMAGE, AND METHOD FOR MEASURING TARGET MOLECULE
Document Type and Number:
WIPO Patent Application WO/2023/106372
Kind Code:
A1
Abstract:
This enzyme measurement method includes: a liquid mixture preparation step in which a liquid mixture containing an enzyme and a selective substrate for the enzyme is prepared; an array preparation step in which a microchamber array with immobilized metal nanostructures that includes a plurality of microchambers in which metal nanostructures are immobilized is prepared; a mixture filling step in which the plurality of microchambers are filled with the liquid mixture so that no more than one molecule of the enzyme is present in each of the plurality of microchambers; and a bright point quantity measurement step in which the microchamber array with immobilized metal nanostructures is illuminated with excitation light and the number of microchambers in which the surface-enhanced Raman scattering intensity derived from an enzymatic reaction product of the selective substrate or from the selective substrate has changed by a value equal to or greater than a preset value is measured, or the number of microchambers in which the surface-enhanced Raman scattering intensity is equal to or greater than a preset threshold value or equal to or less than the threshold value is measured.
Inventors:
WATANABE RIKIYA (JP)
ANDO JUN (JP)
ANDO JUN (JP)
Application Number:
PCT/JP2022/045297
Publication Date:
June 15, 2023
Filing Date:
December 08, 2022
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
G01N21/65
Domestic Patent References:
| WO2010016267A1 | 2010-02-11 |
Foreign References:
| JP2011511933A | 2011-04-14 | |||
| JP2021120646A | 2021-08-19 |
Other References:
LI ET AL.: "A practical wide-field Raman imaging method with high spectral and spatial resolution", REVIEW OF SCIENTIFIC INSTRUMENTS, vol. 89, 2018, pages 083103 - 1-083103-5, XP012230541, DOI: 10.1063/1.5041529
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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