Title:
ENZYME PREPARATION FOR USE IN MEASUREMENT OF GLUCOSE
Document Type and Number:
WIPO Patent Application WO/2016/163448
Kind Code:
A1
Abstract:
[Problem] To provide GDH increased in the applicability to a glucose sensor. [Solution] A composition containing FADGDH, said composition having such a property that, when 0.1 ml of the composition is added to 2.9 ml of a solution containing 10 mM of trehalose and 1 mmol/L of potassium ferricyanide so that the resultant solution can have a glucose dehydrogenase activity of 500 U/ml and subsequently the resultant solution is incubated at 37°C, the degree of decrease in absorbance at 405 nm, which arises from the reduction of potassium ferricyanide, is less than 20 mAbs per minute.
Inventors:
UTASHIMA YUU (JP)
KISHIMOTO TAKAHIDE (JP)
KISHIMOTO TAKAHIDE (JP)
Application Number:
PCT/JP2016/061370
Publication Date:
October 13, 2016
Filing Date:
April 07, 2016
Export Citation:
Assignee:
TOYO BOSEKI (JP)
International Classes:
C12Q1/32; C12M1/34; C12N9/04; C12Q1/54; G01N27/327
Domestic Patent References:
| WO2010126139A1 | 2010-11-04 | |||
| WO2008001903A1 | 2008-01-03 | |||
| WO2007116710A1 | 2007-10-18 | |||
| WO2016035849A1 | 2016-03-10 | |||
| WO2009069381A1 | 2009-06-04 | |||
| WO2006101239A1 | 2006-09-28 | |||
| WO2013147206A1 | 2013-10-03 | |||
| WO2000055349A1 | 2000-09-21 |
Foreign References:
| JP2010057427A | 2010-03-18 | |||
| JP2010054503A | 2010-03-11 | |||
| JP2011139677A | 2011-07-21 | |||
| JP2003261591A | 2003-09-19 | |||
| JP2001233817A | 2001-08-28 |
Other References:
WHITE,J.W. ET AL.: "A glucose oxidase reagent for maltase assay", ANAL. BIOCHEM., vol. 2, no. 4, 1961, pages 380 - 384, XP024824652
UTASHIMA, Y. ET AL.: "Heterologous expression of FAD-dependent glucose dehydrogenase from Aspergillus oryzae in a basidiomycete yeast Cryptococcus sp. S-2", ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 65th, 2013, pages 28
FRIEDMAN, S.: "The purification and properties of trehalase isolated from Phormia regina", MEIG, ARCH. BIOCHEM. BIOPHYS., vol. 87, no. 2, 1960, pages 252 - 258, XP024805258
BAK, T.-G.: "Studies on glucose dehydrogenase of Aspergillus oryzae. II. Purification and physical and chemical properties", BIOCHIM. BIOPHYS. ACTA, vol. 139, no. 2, 1967, pages 277 - 293, XP023510222
BAK, T.-G. ET AL.: "Studies on the glucose dehydrogenase of Aspergillus oryzae. I. Induction of its synthesis by p-benzoquinone and hydroquinone", BIOCHIM. BIOPHYS. ACTA, vol. 139, 1967, pages 265 - 276, XP023510221
UTASHIMA, Y. ET AL.: "Heterologous expression of FAD-dependent glucose dehydrogenase from Aspergillus oryzae in a basidiomycete yeast Cryptococcus sp. S-2", ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, vol. 65th, 2013, pages 28
FRIEDMAN, S.: "The purification and properties of trehalase isolated from Phormia regina", MEIG, ARCH. BIOCHEM. BIOPHYS., vol. 87, no. 2, 1960, pages 252 - 258, XP024805258
BAK, T.-G.: "Studies on glucose dehydrogenase of Aspergillus oryzae. II. Purification and physical and chemical properties", BIOCHIM. BIOPHYS. ACTA, vol. 139, no. 2, 1967, pages 277 - 293, XP023510222
BAK, T.-G. ET AL.: "Studies on the glucose dehydrogenase of Aspergillus oryzae. I. Induction of its synthesis by p-benzoquinone and hydroquinone", BIOCHIM. BIOPHYS. ACTA, vol. 139, 1967, pages 265 - 276, XP023510221
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