Title:
ENZYMES, MICRO-ORGANISMS AND USES THEREOF, AND A METHOD OF DEGRADING A POLYOLEFIN
Document Type and Number:
WIPO Patent Application WO/2022/219239
Kind Code:
A3
Abstract:
The present invention relates to the fields of life sciences, micro-organisms and degradation of polyolefin polymers. Specifically, the invention relates to an isolated specific enzyme or a fragment thereof, wherein said enzyme or fragment is capable of degrading a polyolefin, and to a micro-organism or a host cell comprising the enzyme or a fragment thereof. Also, the present invention relates to a polynucleotide encoding the enzyme or fragment thereof, and to an expression vector or plasmid comprising the polynucleotide of the present invention. And still, the present invention relates to use of the enzyme, fragment, micro-organism, host cell, polynucleotide, expression vector or plasmid of the present invention for degrading a polyolefin; to a method of degrading a polyolefin with the specific enzyme or a fragment thereof; and to a method of producing the enzyme or fragment thereof of the present invention.
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Inventors:
KOIVURANTA KARI (FI)
TSITKO IRINA (FI)
CASTILLO SANDRA (FI)
NYGREN HELI (FI)
TSITKO IRINA (FI)
CASTILLO SANDRA (FI)
NYGREN HELI (FI)
Application Number:
PCT/FI2022/050238
Publication Date:
December 22, 2022
Filing Date:
April 12, 2022
Export Citation:
Assignee:
TEKNOLOGIAN TUTKIMUSKESKUS VTT OY (FI)
International Classes:
C12N9/08; B09B3/60; C08J11/10; C12P7/00
Domestic Patent References:
| WO2016097325A1 | 2016-06-23 | |||
| WO2014079844A1 | 2014-05-30 | |||
| WO2008012236A1 | 2008-01-31 |
Other References:
A: "Non-heme chloroperoxidase from Bacillus cereus", 10 February 2021 (2021-02-10), XP055948983, Retrieved from the Internet [retrieved on 20220804]
TAGHAVI NAVID ET AL: "Challenges in biodegradation of non-degradable thermoplastic waste: From environmental impact to operational readiness", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 49, 27 March 2021 (2021-03-27), XP086578734, ISSN: 0734-9750, [retrieved on 20210327], DOI: 10.1016/J.BIOTECHADV.2021.107731
JIAKANG RU ET AL: "Microbial Degradation and Valorization of Plastic Wastes", FRONTIERS IN MICROBIOLOGY, vol. 11, 21 April 2020 (2020-04-21), XP055738857, DOI: 10.3389/fmicb.2020.00442
SUN YUERU ET AL: "Molecular Basis of the General Base Catalysis of an [alpha]/[beta]-Hydrolase Catalytic Triad", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 22, 1 May 2014 (2014-05-01), US, pages 15867 - 15879, XP055948923, ISSN: 0021-9258, DOI: 10.1074/jbc.M113.535641
TAGHAVI NAVID ET AL: "Challenges in biodegradation of non-degradable thermoplastic waste: From environmental impact to operational readiness", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 49, 27 March 2021 (2021-03-27), XP086578734, ISSN: 0734-9750, [retrieved on 20210327], DOI: 10.1016/J.BIOTECHADV.2021.107731
JIAKANG RU ET AL: "Microbial Degradation and Valorization of Plastic Wastes", FRONTIERS IN MICROBIOLOGY, vol. 11, 21 April 2020 (2020-04-21), XP055738857, DOI: 10.3389/fmicb.2020.00442
SUN YUERU ET AL: "Molecular Basis of the General Base Catalysis of an [alpha]/[beta]-Hydrolase Catalytic Triad", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 22, 1 May 2014 (2014-05-01), US, pages 15867 - 15879, XP055948923, ISSN: 0021-9258, DOI: 10.1074/jbc.M113.535641
Attorney, Agent or Firm:
BERGGREN OY (FI)
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