Title:
ENZYMES WHICH PARTICIPATE IN C-TERMINAL AMIDATION, AND PRODUCTION AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/1991/002790
Kind Code:
A1
Abstract:
An enzyme which acts on a peptide C-terminal/glycine adduct of formula (I), (wherein A represents a residue formed by removing an $g(a)-amino or imino group and an $g(a)-carboxyl group from a natural $g(a)-amino acid, and X represents a hydrogen atom or a residue of an amino acid derivative which combines with the N atom via a carbonyl group) to produce a peptide C-terminal/$g(a)-hydroxyglycine adduct of formula (II), (wherein A and X are each as defined above), and another enzyme which acts on the compound of formula (II) to produce a C-terminal amidated peptide of formula (III), wherein A and X are each as defined above.
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Inventors:
IIDA TOSHII (JP)
KAMINUMA TOSHIHIKO (JP)
FUSE YUKA (JP)
TAJIMA MASAHIRO (JP)
YANAGI MITSUO (JP)
KAMINUMA TOSHIHIKO (JP)
FUSE YUKA (JP)
TAJIMA MASAHIRO (JP)
YANAGI MITSUO (JP)
Application Number:
PCT/JP1990/001036
Publication Date:
March 07, 1991
Filing Date:
August 14, 1990
Export Citation:
Assignee:
SHISEIDO CO LTD (JP)
International Classes:
C07K1/107; C12N9/02; C12N15/53; C12N15/60; (IPC1-7): C12N9/02; C12N9/88
Other References:
J. Am. Chem. Soc., Vol. 111, (1989), S. D. YOUNG, et al. (Enzymatic Peptidyl alfa-Amidating Proceed through Formation of an alfa-Hydoxyglycine Intermidiate) p. 1933-1934.
Biochemical and Biophysical Research Communication, Vol. 151, No. 1, (1988), A.G. KATOPODIS, et al. (A New Facile Trinitpophenylated Substrated for Peptide alfa-Amidaton and Its Use to Characterize PAM Activity in Chromaffin Granules) p. 499-505.
Biochemistry, Vol. 29, No. 26, (1990), A.G. KATOPODIS, et al. (A Novel Enzyme from Bovine Neurointermediate Pituitary Catalyzes Dealkylation of alfa-Hydroxyglycine Derivatives, Thereby Functioning Sequentially with Peptidylglycine alfa-Amidating Monooxygenaze in Peptide Amidation) p. 6115-6120.
Molecular Endocrinology, Vol. 1, No. 11, (1987), B.A. EIPPER, et al. (Structure of the Precursor to an Enzmc Mediating COOH-Tevminal Amidating in Peptide Biosynthesis) p. 777-790.
See also references of EP 0438600A4
Biochemical and Biophysical Research Communication, Vol. 151, No. 1, (1988), A.G. KATOPODIS, et al. (A New Facile Trinitpophenylated Substrated for Peptide alfa-Amidaton and Its Use to Characterize PAM Activity in Chromaffin Granules) p. 499-505.
Biochemistry, Vol. 29, No. 26, (1990), A.G. KATOPODIS, et al. (A Novel Enzyme from Bovine Neurointermediate Pituitary Catalyzes Dealkylation of alfa-Hydroxyglycine Derivatives, Thereby Functioning Sequentially with Peptidylglycine alfa-Amidating Monooxygenaze in Peptide Amidation) p. 6115-6120.
Molecular Endocrinology, Vol. 1, No. 11, (1987), B.A. EIPPER, et al. (Structure of the Precursor to an Enzmc Mediating COOH-Tevminal Amidating in Peptide Biosynthesis) p. 777-790.
See also references of EP 0438600A4
Attorney, Agent or Firm:
Aoki, Akira (Seiko Toranomon Bldg. 8-10, Toranomon 1-chom, Minato-ku Tokyo 105, JP)
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