EPITOPE-TAGGED RECOMBINANT GROWTH ARREST SPECIFIC GENE 6 PROTEIN

WIPO Patent Application WO2004029209

A3

The present invention provides epitope-tagged mammalian recombinant Growth Arrest Specific Gene 6 (Gas6) proteins or polypeptides that retain their biological activity of Gas6, including a novel method of generating and purifying the aforesaid proteins or polypeptides. Such biolgically active epitope-tagged Gas6 proteins or polypeptides can be used when the presence of Gas6 is required and/or when Gas6 antagonists and receptors need to be identified. Prior to being expressed from a mammalian cell, the recombinant Gas6 protein or polypeptide is tagged with either Flag or polyhistidine to the C-terminus. After expression, the protein or polypeptide is purified in a single step affinity purification using either anti-Flag antibody resin or nickle resin. The purified epitope-tagged mammalian recombinant Gas6 protein or polypeptide maintains its biological activity and can be used thereafter.

WO1998042296	COMPOSITIONS AND METHODS FOR DIAGNOSING/TREATING DISEASE BASED ON BETA-CATENIN/TRANSCRIPTION FACTOR INTERACTIONS
WO/2020/218823	COMPOSITION CONTAINING, AS ACTIVE INGREDIENT, KLOTHO PROTEINS OR GENES ENCODING SAME
JP2937481	[Title of the Invention] Oligonucleotide regulation of multi-drug resistance-related proteins

YANG JING (US)
CHEN CAILIN (US)
HU BING (US)
ANDRADE-GORDON PATRICIA (US)
JORDAN ROBERT (US)

PCT/US2003/030330

January 27, 2005

September 24, 2003

Click for automatic bibliography generation  

CENTOCOR INC (US)
YANG JING (US)
CHEN CAILIN (US)
HU BING (US)
ANDRADE-GORDON PATRICIA (US)
JORDAN ROBERT (US)

C07K14/47; (IPC1-7): A61K38/00

US6169070B1

2001-01-02

View/Download PDF      PDF Help

Previous Patent: CELL SEPARATION COMPOSITIONS AND METHODS

Next Patent: RESOLUTION OF AGGRECAN GENE EXPRESSION USING SPECIFIC AND SELECTIVE ELECTRICAL AND ELECTROMAGNETIC S...