Title:
ERGOTHIONEINE PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2019/163767
Kind Code:
A1
Abstract:
Provided are a method for producing ergothioneine in high yield at low cost and a bacterium for use in the method. This method is designed to produce ergothioneine, a related substance thereof, or a mixture of these, and is characterized by comprising culturing, in a medium, an Enterobacteriaceae bacterium capable of producing ergothioneine, and collecting ergothioneine, a related substance thereof, or a mixture of these from the medium or cultured cells, and in that the bacterium is a strain that is modified such that the activity of a protein containing a core sequence region having 5 amino acids Ser-Arg-Gly-Arg-Thr (SEQ ID NO: 7) is reduced. This bacterium is an Enterobacteriaceae bacterium that is capable of producing ergothioneine, and is modified such that the activity of a protein containing a core sequence region having 5 amino acids Ser-Arg-Gly-Arg-Thr (SEQ ID NO: 7) is reduced.
Inventors:
NAKATANI TAKESHI (JP)
NAKASHIMA NANAMI (JP)
NAKASHIMA NANAMI (JP)
Application Number:
PCT/JP2019/006089
Publication Date:
August 29, 2019
Filing Date:
February 19, 2019
Export Citation:
Assignee:
NAGASE & CO LTD (JP)
International Classes:
C12P13/04; C12N1/21; C12N15/31
Domestic Patent References:
| WO2015168112A1 | 2015-11-05 | |||
| WO2016121285A1 | 2016-08-04 | |||
| WO2017150304A1 | 2017-09-08 | |||
| WO2017150304A1 | 2017-09-08 |
Foreign References:
| JP2016502859A | 2016-02-01 | |||
| JP2017225368A | 2017-12-28 | |||
| JP2006160748A | 2006-06-22 | |||
| JP2007300916A | 2007-11-22 |
Other References:
JONES, GARY W. ET AL.: "The evolutionary history of the genes involved in the biosynthesis of the antioxidant ergothioneine", GENE, vol. 549, 2014, pages 161 - 170, XP029044015, doi:10.1016/j.gene.2014.07.065
L. SERVILLO ET AL., FREE. RADIC. BIOL. MED., vol. 79, 2015, pages 228 - 36
R. M. Y. TANG ET AL., SCI. REP., vol. 8, no. 1, 2018, pages 1601
F. P. SEEBECK, J. AM. CHEM. SOC., vol. 132, 2010, pages 6632 - 6633
T. PLUSKAL ET AL., PLOS ONE., vol. 9, no. 5, 2014, pages e97774
W. HU ET AL., ORG. LETT., vol. 16, 2014, pages 5382 - 5385
S. NAKAJIMA ET AL., J. BIOSCI. BIOENG., vol. 120, 2015, pages 294 - 298
R. BURN ET AL., ANGEW. CHEM. INT. EDIT., vol. 56, 2017, pages 12508 - 12511
G. W. JONES ET AL., GENE, vol. 549, 2014, pages 161 - 170
M. S. DONNENBERGJ. S. KAPER, INFECT. IMMUN., 1991, pages 4310 - 4317
H. MIZOGUCHI ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 71, no. 12, 2007, pages 2905 - 2911
See also references of EP 3751001A4
L. SERVILLO ET AL., FREE. RADIC. BIOL. MED., vol. 79, 2015, pages 228 - 36
R. M. Y. TANG ET AL., SCI. REP., vol. 8, no. 1, 2018, pages 1601
F. P. SEEBECK, J. AM. CHEM. SOC., vol. 132, 2010, pages 6632 - 6633
T. PLUSKAL ET AL., PLOS ONE., vol. 9, no. 5, 2014, pages e97774
W. HU ET AL., ORG. LETT., vol. 16, 2014, pages 5382 - 5385
S. NAKAJIMA ET AL., J. BIOSCI. BIOENG., vol. 120, 2015, pages 294 - 298
R. BURN ET AL., ANGEW. CHEM. INT. EDIT., vol. 56, 2017, pages 12508 - 12511
G. W. JONES ET AL., GENE, vol. 549, 2014, pages 161 - 170
M. S. DONNENBERGJ. S. KAPER, INFECT. IMMUN., 1991, pages 4310 - 4317
H. MIZOGUCHI ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 71, no. 12, 2007, pages 2905 - 2911
See also references of EP 3751001A4
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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