Title:
ERROR CONFIRMATION METHOD AND DEVICE FOR MASSIVE PARALLEL SEQUENCING
Document Type and Number:
WIPO Patent Application WO/2017/179946
Kind Code:
A1
Abstract:
Provided is an error confirmation method for massive parallel sequencing, comprising the steps of: (a) selecting at least one first read to be verified, among reads generated by massive parallel sequencing; (b) recovering, from a sequencing plate, hexane fragments corresponding to the first read; (c) performing sequencing for the respective fragments or an amplification product thereof so as to generate a second read; and (d) comparing the second read with the first read corresponding thereto so as to verify an error of the first read. In addition, provided is an error confirmation device, which is for massive parallel sequencing and performing the method.
Inventors:
KWON SUNG HOON (KR)
YEOM HUI RAN (KR)
RYU TAE HOON (KR)
LEE YONG HEE (KR)
KIM JUNG MIN (KR)
NOH JIN SUNG (KR)
YEOM HUI RAN (KR)
RYU TAE HOON (KR)
LEE YONG HEE (KR)
KIM JUNG MIN (KR)
NOH JIN SUNG (KR)
Application Number:
PCT/KR2017/004067
Publication Date:
October 19, 2017
Filing Date:
April 14, 2017
Export Citation:
Assignee:
UNIV SEOUL NAT R & DB FOUND (KR)
International Classes:
C12Q1/68; G16B20/20
Foreign References:
| JP2013226104A | 2013-11-07 |
Other References:
SCHMITT ET AL.: "Detection of Ultra-rare Mutations by Next-generation Sequencing", PNAS, vol. 109, no. 36, 2012, pages 14508 - 14513, XP055161683
ROBASKY ET AL.: "The Role of Replicates for Error Mitigation in Next-generation Sequencing", NATURE REVIEWS GENETICS, vol. 15, no. 1, 2014, pages 56 - 62, XP055433408
ZAGORDI ET AL.: "Error Correction of Next-generation Sequencing Data and Reliable Estimation of HIV Quasispecies", NUCLEIC ACIDS RESEARCH, vol. 38, no. 21, 2010, pages 7400 - 7409, XP055157963
FLAHERTY ET AL.: "Ultrasensitive Detection of Rare Mutations Using Next-generation Targeted Resequencing", NUCLEIC ACIDS RESEARCH, vol. 40, no. 1, XP055161679
LOU ET AL.: "High-throughput DNA Sequencing Errors are Reduced by Orders of Magnitude Using Circle Sequencing", PNAS, vol. 110, no. 49, 2013, pages 19872 - 19877, XP055374686
ROBASKY ET AL.: "The Role of Replicates for Error Mitigation in Next-generation Sequencing", NATURE REVIEWS GENETICS, vol. 15, no. 1, 2014, pages 56 - 62, XP055433408
ZAGORDI ET AL.: "Error Correction of Next-generation Sequencing Data and Reliable Estimation of HIV Quasispecies", NUCLEIC ACIDS RESEARCH, vol. 38, no. 21, 2010, pages 7400 - 7409, XP055157963
FLAHERTY ET AL.: "Ultrasensitive Detection of Rare Mutations Using Next-generation Targeted Resequencing", NUCLEIC ACIDS RESEARCH, vol. 40, no. 1, XP055161679
LOU ET AL.: "High-throughput DNA Sequencing Errors are Reduced by Orders of Magnitude Using Circle Sequencing", PNAS, vol. 110, no. 49, 2013, pages 19872 - 19877, XP055374686
Attorney, Agent or Firm:
KWAK, Hyun Kyu et al. (KR)
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