Title:
ESCHERICHIA COLI EXPRESSION VECTOR AND THE USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2007/016860
Kind Code:
A1
Abstract:
The present invention discloses Escherichia coli expression vector, as well as
a method for steadily, high-efficiently expressing a required target protein in
Escherichia coli using the expression vector. The expression vector is constructed from
vector pRSET-A, wherein ampicillin resistance gene in said vector pRSET-A is
replaced by kanamycin resistance gene. The expression vector can be used to high-efficiently
express enzyme required for the production of 7-aminocephalosporanic acid,
meanwhile it does not produce β-lactamase by which the productivity of
7-aminocephalosporanic acid is reduced. The present invention also discloses two
expression vectors, i.e., one expression vector pHS-GHA whose gene product is
D-amino acid oxidase mutant GHA, and another expression vector pT7-kan-ACY whose
gene product is glutaryl-7-aminocephalosporanic acid acylase of Pseudomonas sp.
SE83.
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Inventors:
WANG JUN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
LIU ZHAOMING (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
LIU ZHAOMING (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)
Application Number:
PCT/CN2006/001939
Publication Date:
February 15, 2007
Filing Date:
August 02, 2006
Export Citation:
Assignee:
BIORIGHT WORLDWIDE CO LTD
WANG JUN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
LIU ZHAOMING (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)
WANG JUN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
LIU ZHAOMING (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)
International Classes:
C12N15/70; C12N9/06; C12N9/14; C12N15/68; C12N15/72; C12P21/02; C12P35/06
Foreign References:
US5922583A | 1999-07-13 | |||
CN1680558A | 2005-10-12 | |||
CN1680583A | 2005-10-12 | |||
EP0364275A2 | 1990-04-18 |
Other References:
LONG-LIU LIN ET AL.: "Expression of trigonopsis variabilis D-amino acid oxidase gene in escherichia coli and characterization of its inactive mutants", ENZYME AND MICROBIAL TECHNOLOGY, vol. 27, 2000, pages 482 - 491, XP002253416
See also references of EP 1925670A4
See also references of EP 1925670A4
Attorney, Agent or Firm:
TEE & HOWE INTELLECTUAL PROPERTY ATTORNEY (Room 718 Beijing Capital Times Square, 88 Xichang'an Avenu, Xicheng District 1 Beijing, CN)
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