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Title:
ESCHERICHIA COLI EXPRESSION VECTOR AND THE USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2007/016860
Kind Code:
A1
Abstract:
The present invention discloses Escherichia coli expression vector, as well as a method for steadily, high-efficiently expressing a required target protein in Escherichia coli using the expression vector. The expression vector is constructed from vector pRSET-A, wherein ampicillin resistance gene in said vector pRSET-A is replaced by kanamycin resistance gene. The expression vector can be used to high-efficiently express enzyme required for the production of 7-aminocephalosporanic acid, meanwhile it does not produce β-lactamase by which the productivity of 7-aminocephalosporanic acid is reduced. The present invention also discloses two expression vectors, i.e., one expression vector pHS-GHA whose gene product is D-amino acid oxidase mutant GHA, and another expression vector pT7-kan-ACY whose gene product is glutaryl-7-aminocephalosporanic acid acylase of Pseudomonas sp. SE83.

Inventors:
WANG JUN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
LIU ZHAOMING (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)
Application Number:
PCT/CN2006/001939
Publication Date:
February 15, 2007
Filing Date:
August 02, 2006
Export Citation:
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Assignee:
BIORIGHT WORLDWIDE CO LTD
WANG JUN (CN)
TSANG WAIKEI (CN)
YAP HONGKIN (CN)
LIU ZHAOMING (CN)
SIU YAULUNG (CN)
TSANG SUPYIN (CN)
International Classes:
C12N15/70; C12N9/06; C12N9/14; C12N15/68; C12N15/72; C12P21/02; C12P35/06
Foreign References:
US5922583A1999-07-13
CN1680558A2005-10-12
CN1680583A2005-10-12
EP0364275A21990-04-18
Other References:
LONG-LIU LIN ET AL.: "Expression of trigonopsis variabilis D-amino acid oxidase gene in escherichia coli and characterization of its inactive mutants", ENZYME AND MICROBIAL TECHNOLOGY, vol. 27, 2000, pages 482 - 491, XP002253416
See also references of EP 1925670A4
Attorney, Agent or Firm:
TEE & HOWE INTELLECTUAL PROPERTY ATTORNEY (Room 718 Beijing Capital Times Square, 88 Xichang'an Avenu, Xicheng District 1 Beijing, CN)
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