Title:
ESCHERICHIA COLI JLTRP STRAIN AND APPLICATION THEREOF IN L-TRYPTOPHAN SYNTHESIS
Document Type and Number:
WIPO Patent Application WO/2017/197887
Kind Code:
A1
Abstract:
An Escherichia coli JLTrp strain and application thereof in L-tryptophan synthesis. The strain has a culture collection number of CGMCC No.7.217 and is resistant against multiple antibiotics. The method using the strain for fermentation-production of L-tryptophan comprises the following steps: (1) growing a mature Escherichia coli JLTrp bacterial culture; and (2) fermentation tank culturing: inoculating the mature bacterial culture in a fermentation tank, and performing fermentation-production and extraction of L-tryptophan at a temperature of 34-36°C, stage-based pH control by adding liquid ammonia, a pressure of 0.03-0.08 MPa, an aeration rate of 0.3-2.0 VVM, and a residual sugar level maintained at 0.01-0.5% during the fermentation process. The L-tryptophan produced using the strain has an increased concentration of 70-80 g/L of L-tryptophan.
Inventors:
LIU SHUAI (CN)
CAO HUAJIE (CN)
CAO HUAJIE (CN)
Application Number:
PCT/CN2016/112363
Publication Date:
November 23, 2017
Filing Date:
December 27, 2016
Export Citation:
Assignee:
HENAN JULONG BIOLOGICAL ENG CO LTD (CN)
International Classes:
C12N1/20; C12P13/22; C12R1/19
Domestic Patent References:
WO2012099396A2 | 2012-07-26 |
Foreign References:
CN105861380A | 2016-08-17 | |||
CN105861587A | 2016-08-17 | |||
CN105925633A | 2016-09-07 | |||
CN104185679A | 2014-12-03 | |||
CN101323868A | 2008-12-17 |
Other References:
LIAO, WEIHONG ET AL.: "da4chang2gan3junlfaljiao4shenglchan3 L-se4an1suan1gong1yi4jian3xi1", BIOLOGY TECHNOLOGY WORLD, 30 April 2016 (2016-04-30), ISSN: 1674-2060
ZHAO ZHI-JUN ET AL.: "Development of L-tryptophan production strains by defined genetic modification in Escherichia coli", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 38, no. 12, 31 December 2011 (2011-12-31), pages 1921 - 1929, XP019982205, ISSN: 1367-5435
ZHAO ZHI-JUN ET AL.: "Development of L-tryptophan production strains by defined genetic modification in Escherichia coli", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 38, no. 12, 31 December 2011 (2011-12-31), pages 1921 - 1929, XP019982205, ISSN: 1367-5435
Attorney, Agent or Firm:
LUOYANG GONGXIN IP AGENCY (CN)
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