Title:
EURAKYOTIC SECRETION SIGNAL SEQUENCE FOR RECOMBINANT EXPRESSION IN PROKARYOTES
Document Type and Number:
WIPO Patent Application WO/2009/147382
Kind Code:
A3
Abstract:
An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.
Inventors:
KARA BHUPENDRA VALLABH (GB)
LENNON CHRISTOPHER DAVID JOHN (GB)
LENNON CHRISTOPHER DAVID JOHN (GB)
Application Number:
PCT/GB2009/001372
Publication Date:
February 24, 2011
Filing Date:
June 01, 2009
Export Citation:
Assignee:
MSD BIOLOG UK LTD (GB)
KARA BHUPENDRA VALLABH (GB)
LENNON CHRISTOPHER DAVID JOHN (GB)
KARA BHUPENDRA VALLABH (GB)
LENNON CHRISTOPHER DAVID JOHN (GB)
International Classes:
C12N15/62; C12N15/70
Domestic Patent References:
| WO2003068956A1 | 2003-08-21 |
Other References:
HUMPHREYS D P ET AL: "High-Level Periplasmic Expression in Escherichia coli Using a Eukaryotic Signal Peptide: Importance of Codon Usage at the 5' End of the Coding Sequence", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 20, no. 2, 1 November 2000 (2000-11-01), pages 252 - 264, XP004435483, ISSN: 1046-5928
BELIN D ET AL: "Functional Activity of Eukaryotic Signal Sequences in Escherichia coli: the Ovalbumin Family of Serine Protease Inhibitors", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 335, no. 2, 9 January 2004 (2004-01-09), pages 437 - 453, XP004479234, ISSN: 0022-2836
BELIN D ET AL: "Functional Activity of Eukaryotic Signal Sequences in Escherichia coli: the Ovalbumin Family of Serine Protease Inhibitors", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 335, no. 2, 9 January 2004 (2004-01-09), pages 437 - 453, XP004479234, ISSN: 0022-2836
Attorney, Agent or Firm:
REVELL, Christopher (PO Box 42 Hexagon Tower,Blackley, Manchester M9 8ZS, GB)
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