Title:
EXO-β-1,2-GLUCANASE AND METHOD FOR PRODUCING SOPHOROSE
Document Type and Number:
WIPO Patent Application WO/2018/194092
Kind Code:
A1
Abstract:
Provided is exo-β-1,2-glucanase which is one member selected from among: exo-β-1,2-glucanase acting on linear β-1,2-glucan to form sophorose; (a) a protein comprising the amino acid sequence of SEQ ID NO: 1; (b) a protein comprising an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by deletion of the amino acid residues at the 1-18 positions; (c) a protein comprising an amino acid sequence derived from the amino acid sequence of (a) or (b) by substitution, deletion or addition of one to several amino acid residues and having exo-β-1,2-glucanase activity; and (d) a protein showing a 90% or more sequence identity to the amino acid sequence of (a) or (b) and having exo-β-1,2-glucanase activity. Also provided is a method for producing sophorose with the use of exo-β-1,2-glucanase.
Inventors:
NAKAJIMA MASAHIRO (JP)
TAGUCHI HAYAO (JP)
SHIMIZU HISAKA (JP)
NAKAI HIROYUKI (JP)
TAGUCHI HAYAO (JP)
SHIMIZU HISAKA (JP)
NAKAI HIROYUKI (JP)
Application Number:
PCT/JP2018/016001
Publication Date:
October 25, 2018
Filing Date:
April 18, 2018
Export Citation:
Assignee:
UNIV TOKYO SCIENCE FOUND (JP)
UNIV NIIGATA (JP)
UNIV NIIGATA (JP)
International Classes:
C12N15/31; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/24; C12N15/52; C12N15/63; C12P19/12
Foreign References:
| JPS59154985A | 1984-09-04 |
Other References:
DATABASE Protein [online] 31 January 2014 (2014-01-31), "Conserved hypothetical protein [Parabacteroides distasonis ATCC 8503", XP055613360, retrieved from NCBI Database accession no. ABR44770.1
XU, JIAN ET AL.: "Evolution of symbiotic bacteria in the distal human intestine", PLOS BIOLOGY, vol. 5, no. 7, 2007, pages 1575 1585 23 - 27, XP055103941
REESE, ELWYN T. ET AL.: "beta-D-1,2-GLUCANASES IN FUNGI", CANADIAN JOURNAL OF MICROBIOLOGY, vol. 7, no. 3, 1961, pages 309 - 317
TANAKA, NOBUKIYO ET AL.: "Isolation and identification of novel 1, 2-beta-glucan degradation enzyme, and functional analysis", LECTURE ABSTRACT IN 2015 MEETING OF JAPAN SOCIETY OF BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 5 March 2015 (2015-03-05)
ABE, KOICHI ET AL.: "Biochemical and structural analyses of a bacterial endo-beta-1, 2-glucanase reveal a new glycoside hydrolase family", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 292, no. 18, 7 March 2017 (2017-03-07), pages 7487 - 7506, XP055613367
XU, JIAN ET AL.: "Evolution of symbiotic bacteria in the distal human intestine", PLOS BIOLOGY, vol. 5, no. 7, 2007, pages 1575 1585 23 - 27, XP055103941
REESE, ELWYN T. ET AL.: "beta-D-1,2-GLUCANASES IN FUNGI", CANADIAN JOURNAL OF MICROBIOLOGY, vol. 7, no. 3, 1961, pages 309 - 317
TANAKA, NOBUKIYO ET AL.: "Isolation and identification of novel 1, 2-beta-glucan degradation enzyme, and functional analysis", LECTURE ABSTRACT IN 2015 MEETING OF JAPAN SOCIETY OF BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY, 5 March 2015 (2015-03-05)
ABE, KOICHI ET AL.: "Biochemical and structural analyses of a bacterial endo-beta-1, 2-glucanase reveal a new glycoside hydrolase family", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 292, no. 18, 7 March 2017 (2017-03-07), pages 7487 - 7506, XP055613367
Attorney, Agent or Firm:
SHOBAYASHI Masayuki (JP)
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