Title:
EXOSOMAL DNA-BASED METHOD FOR PERFORMING NON-INVASIVE PRENATAL DIAGNOSIS AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/137141
Kind Code:
A1
Abstract:
Provided is a method for isolating exosomal DNA, the method comprising the steps of: (i) providing a sample, the sample comprising a blood sample from peripheral blood of a pregnant woman; and (ii) separating the sample so as to obtain exosomal DNA. Also provided are a method for performing detection on a blood sample, a method for constructing a sequencing library for blood samples, a method for high-throughput sequencing of an exosomal DNA sequencing library, a non-invasive prenatal gene detection method, a non-invasive prenatal gene detection device, and a kit for performing detection on a blood sample and a use thereof.
Inventors:
GAO YA (CN)
LU SEN (CN)
ZHAO JIA (CN)
ZENG PENG (CN)
CHEN FANG (CN)
JIANG HUI (CN)
LU SEN (CN)
ZHAO JIA (CN)
ZENG PENG (CN)
CHEN FANG (CN)
JIANG HUI (CN)
Application Number:
PCT/CN2017/072488
Publication Date:
August 02, 2018
Filing Date:
January 24, 2017
Export Citation:
Assignee:
BGI SHENZHEN (CN)
MGI TECH CO LTD (CN)
MGI TECH CO LTD (CN)
International Classes:
C12M1/34; C12N15/10; C12Q1/68; G01N33/53
Domestic Patent References:
| WO2015153732A2 | 2015-10-08 |
Foreign References:
| CN105779586A | 2016-07-20 | |||
| CN105400864A | 2016-03-16 | |||
| CN103403183A | 2013-11-20 |
Other References:
LI, YUJING ET AL.: "Isolation and identification of placental exosomes from maternal serum", JOURNAL OF MEDICAL POSTGRADUATES, vol. 28, no. 6, 15 June 2015 (2015-06-15), pages 632 - 636, XP009515714, ISSN: 1008-8199, DOI: 10.16571/j.cnki.1008-8199.2015.06.017
SABAPATHA, A. ET AL.: "Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences", AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol. 56, no. 5-6, 31 October 2006 (2006-10-31), XP055054551, ISSN: 1046-7408
SAMBROOK ET AL.: "Molecular Cloning: Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3575399A4
SABAPATHA, A. ET AL.: "Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences", AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol. 56, no. 5-6, 31 October 2006 (2006-10-31), XP055054551, ISSN: 1046-7408
SAMBROOK ET AL.: "Molecular Cloning: Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3575399A4
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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