Title:
EXOSOME ACTIVE PREPARATION PROMOTING ENDOTHELIAL CELL ANGIOGENESIS AND PREPARATION METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/210833
Kind Code:
A1
Abstract:
Provided are an exosome active preparation promoting endothelial cell angiogenesis and a preparation method and use thereof. The preparation method comprises the following steps: isolating primary umbilical venous endothelial cells, and carrying out cell culture and passage; adding anisodamine into a subcultured endothelial cell culture solution for pre-treating endothelial cells, and then exchange same for a new endothelial cell culture solution to continuously culture the endothelial cells; extracting an exosome from the endothelial cell culture solution obtained after the culturing of the endothelial cells; and identifying the exosome. The exosome active preparation promoting endothelial cell angiogenesis prepared by the preparation method can be used in the preparation of medicines for treating microvascular injuries, microcirculation disturbances and cardiac and cerebral infarctions.
Inventors:
ZHANG HONGGANG (CN)
ZHANG QIUJU (CN)
LI BINGWEI (CN)
XIU RUIJUAN (CN)
ZHANG QIUJU (CN)
LI BINGWEI (CN)
XIU RUIJUAN (CN)
Application Number:
PCT/CN2019/085071
Publication Date:
November 07, 2019
Filing Date:
April 29, 2019
Export Citation:
Assignee:
INST OF MICROCIRCULATION CHINESE ACADEMY OF MEDICAL SCIENCES & PEKING UNION MEDICAL COLLEGE (CN)
International Classes:
C12N5/071; A61K35/44; A61P9/00; A61P9/10; A61P9/14
Foreign References:
| CN108753682A | 2018-11-06 | |||
| CN107937342A | 2018-04-20 | |||
| US20160108368A1 | 2016-04-21 |
Other References:
YAN, BINGHAO ET AL.: "Preparation and Application of Exosomes Derived from Mesenchymal Stem Cells", CHINESE JOURNAL OF ANATOMY, vol. 39, no. 5, 25 October 2016 (2016-10-25), pages 622 - 625, ISSN: 1001-1633
ZHANG, YZ ET AL.: "Exosomes Derived from Human Umbilical Vein Endothelial Cells Promote Neural Stem Cell Expansion While Maintain Their Sternness in Culture", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 495, no. 1, 15 November 2017 (2017-11-15), pages 892 - 897, XP055648816, ISSN: 0006-291X
ZHANG, YZ ET AL.: "Exosomes Derived from Human Umbilical Vein Endothelial Cells Promote Neural Stem Cell Expansion While Maintain Their Sternness in Culture", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 495, no. 1, 15 November 2017 (2017-11-15), pages 892 - 897, XP055648816, ISSN: 0006-291X
Attorney, Agent or Firm:
KEYCOM PARTNERS, P.C. (CN)
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