Title:
EXOSOME MEASUREMENT METHOD AND EXOSOME MEASUREMENT KIT
Document Type and Number:
WIPO Patent Application WO/2020/262374
Kind Code:
A1
Abstract:
The present invention provides an exosome measurement method that makes it possible to measure the exosome concentration in a sample with higher sensitivity and more easily compared with existing measurement methods. In the measurement method according to the present invention, a measurement chip including a metallic film and a first binding substance that is immobilized on the metallic film and that binds to exosomes is prepared. A sample containing exosomes is provided on the metallic film, causing the exosomes contained in the sample to bind to the first binding substance. The exosomes are labelled with a fluorescent substance via a second binding substance that binds to the exosomes. In a state where the exosomes labelled with the fluorescent substance are bound to the first binding substance, the metallic film is irradiated with excitation light so as to cause surface plasmon resonance on the metallic film, and fluorescence radiated from the fluorescent substance is detected.
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Inventors:
AOKI YOUICHI
KAYA TAKATOSHI
KAYA TAKATOSHI
Application Number:
PCT/JP2020/024585
Publication Date:
December 30, 2020
Filing Date:
June 23, 2020
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
G01N21/65; G01N33/53; G01N33/543
Foreign References:
| US20160334398A1 | 2016-11-17 | |||
| JP2016125937A | 2016-07-11 | |||
| JP2019050737A | 2019-04-04 | |||
| JP2014522993A | 2014-09-08 | |||
| JP2015230280A | 2015-12-21 |
Other References:
ZHU, LING ET AL.: "Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging", ANALYTICAL CHEMISTRY, vol. 86, no. 17, 2014, pages 8857 - 8864, XP055368046, DOI: 10.1021/ac5023056
Attorney, Agent or Firm:
WASHIDA & ASSOCIATES (JP)
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