Title:
EXOSOME PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2020/027185
Kind Code:
A1
Abstract:
An exosome production method comprising: (i) a step for ultrafiltering a sample containing at least one exosome; and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.
Inventors:
SHIKU HIROSHI (JP)
SEO NAOHIRO (JP)
KANEDA TSUGUHIRO (JP)
NAKAMURA JUNKO (JP)
AKIYOSHI KAZUNARI (JP)
SEO NAOHIRO (JP)
KANEDA TSUGUHIRO (JP)
NAKAMURA JUNKO (JP)
AKIYOSHI KAZUNARI (JP)
Application Number:
PCT/JP2019/029972
Publication Date:
February 06, 2020
Filing Date:
July 31, 2019
Export Citation:
Assignee:
UNIV MIE (JP)
International Classes:
G01N30/02; G01N33/48; G01N30/88
Foreign References:
| JP2002535665A | 2002-10-22 | |||
| US20170143812A1 | 2017-05-25 | |||
| JP2018501221A | 2018-01-18 |
Other References:
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COLOMBO MMOITA CVAN NIEL GKOWAL JVIGNERON JBENAROCH PMANEL NMOITA LFTHERY CRAPOSO G: "Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles", J CELL SCI, vol. 126, 2013, pages 5553 - 5565, XP055286174, DOI: 10.1242/jcs.128868
SETTE PJADWIN JADUSSUPT VBELLO NFBOUAMR F: "The ESCRT-associated protein Alix recruits the ubiquitin ligase Nedd4-1 to facilitate HIV-1 release through the LYPXnL L domain motif", J VIROL, vol. 84, no. 16, 2010, pages 8181 - 8192
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VAN NIEL GCHARRIN SSIMOES SROMAO MROCHIN LSAFTIG PMARKS MSRUBINSTEIN ERAPOSO G: "The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis", DEV CELL, vol. 21, no. 4, 2011, pages 708 - 721, XP028320002, DOI: 10.1016/j.devcel.2011.08.019
PALMA JYADDANAPUDI SCPIGATI LHAVENS MAJEONG SWEINER GAWEIMER KMSTERN BHASTINGS MLDUELLI DM: "MicroRNAs are exported from malignant cells in customized particles", NUCLEIC ACIDS RES, vol. 40, no. 18, 2012, pages 9125 - 9138, XP055292912, DOI: 10.1093/nar/gks656
ROBBINS PDMORELLI AE: "Regulation of immune responses by extracellular vesicles", NAT REV IMMUNOL, vol. 14, no. 3, 2014, pages 195 - 208, XP055324647, DOI: 10.1038/nri3622
ROYO FFALCON-PEREZ JM: "Liver extracellular vesicles in health and disease", J EXTRACELL VESICLES, vol. 1, 2012, pages 18825
WENDLER FSTAMP GWGIAMAS G: "Tumor-Stromal Cell Communication: Small Vesicles Signal Big Changes", TRENDS CANCER, vol. 2, no. 7, 2016, pages 326 - 329
BECKER ATHAKUR BKWEISS JMKIM HSPEINADO HLYDEN D: "Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis", CANCER CELL, vol. 30, no. 6, 2016, pages 836 - 848, XP029845231, DOI: 10.1016/j.ccell.2016.10.009
MULLER LMITSUHASHI MSIMMS PGOODING WEWHITESIDE TL: "Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets", SCI REP, vol. 6, 2016, pages 20254, XP055616614, DOI: 10.1038/srep20254
SEO NSHIRAKURA YTAHARA YMOMOSE FHARADA NIKEDA HAKIYOSHI KSHIKU H: "Activated CD8+ T cell extracellular vesicles prevent tumour progression by targeting of lesional mesenchymal cells", NAT COMMUN, vol. 9, no. 1, 2018, pages 435
ESTELLES ASPERINDE JROULON TAGUILAR BBONNER CLEPECQ JBDELCAYRE A: "Exosome nanovesicles displaying G protein-coupled receptors for drug discovery", INT J NANOMEDICINE, vol. 2, no. 4, 2007, pages 751 - 760
WUBBOLTS RLECKIE RSVEENHUIZEN PTSCHWARZMANN GMOBIUS WHOERNSCHEMEYER JSLOT JWGEUZE HJSTOORVOGEL W: "Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation", J BIOL CHEM, vol. 278, no. 13, 2003, pages 10963 - 10972, XP055196513, DOI: 10.1074/jbc.M207550200
DE GASSART AGEMINARD CFEVRIER BRAPOSO GVIDAL M: "Lipid raft-associated protein sorting in exosomes", BLOOD, vol. 102, no. 13, 2003, pages 4336 - 4344
MATSUMOTO ATAKAHASHI YNISHIKAWA MSANO KMORISHITA MCHAROENVIRIYAKUL CSAJI HTAKAKURA Y: "Role of Phosphatidylserine-Derived Negative Surface Charges in the Recognition and Uptake of Intravenously Injected B 16BL6-Derived Exosomes by Macrophages", J PHARM SCI, vol. 106, no. 1, 2017, pages 168 - 175
PAROLINI IFEDERICI CRAGGI CLUGINI LPALLESCHI SDE MILITO ACOSCIA CIESSI ELOGOZZI MMOLINARI A: "Microenvironmental pH is a key factor for exosome traffic in tumor cells", J BIOL CHEM, vol. 284, no. 49, 2009, pages 34211 - 34222, XP055201785, DOI: 10.1074/jbc.M109.041152
LOBB RJBECKER MWEN SWWONG CSWIEGMANS APLEIMGRUBER AMOLLER A: "Optimized exosome isolation protocol for cell culture supernatant and human plasma", J EXTRACELL VESICLES, vol. 4, 2015, pages 27031, XP055279331, DOI: 10.3402/jev.v4.27031
MOMEN-HERAVI FBALAJ LALIAN SMANTEL PYHALLECK AETRACHTENBERG AJSORIA CEOQUIN SBONEBREAK CMSARACOGLU E: "Current methods for the isolation of extracellular vesicles", BIOL CHEM, vol. 394, no. 10, 2013, pages 1253 - 1262, XP009191063
BARANYAI THERCZEG KONODI ZVOSZKA IMODOS KMARTON NNAGY GMAGER IWOOD MJEL ANDALOUSSI S: "Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods", PLOS ONE, vol. 10, no. 12, 2015, pages e0145686, XP055406112, DOI: 10.1371/journal.pone.0145686
WILLMS EJOHANSSON HJMAGER ILEE YBLOMBERG KESADIK MALAARG ASMITH CILEHTIO JEL ANDALOUSSI S: "Cells release subpopulations of exosomes with distinct molecular and biological properties", SCI REP, vol. 6, 2016, pages 22519
XU RGREENING DWZHU HJTAKAHASHI NSIMPSON RJ: "Extracellular vesicle isolation and characterization: toward clinical application", J CLIN INVEST, vol. 126, no. 4, 2016, pages 1152 - 1162, XP055500209, DOI: 10.1172/JCI81129
ZHANG ZWANG CLI TLIU ZLI L: "Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line-derived exosomes", ONCOL LETT, vol. 8, no. 4, 2014, pages 1701 - 1706
STRANSKA RGYSBRECHTS LWOUTERS JVERMEERSCH PBLOCH KDIERICKX DANDREI GSNOECK R: "Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma", J TRANSL MED, vol. 16, no. 1, 2018, pages 1, XP055590010, DOI: 10.1186/s12967-017-1374-6
NAKAI WYOSHIDA TDIEZ DMIYATAKE YNISHIBU TIMAWAKA NNARUSE KSADAMURA YHANAYAMA R: "A novel affinity-based method for the isolation of highly purified extracellular vesicles", SCI REP, vol. 6, 2016, pages 33935, XP055561243, DOI: 10.1038/srep33935
GYORGY BSZABO TGPASZTOI MPAL ZMISJAK PARADI BLASZLO VPALLINGER EPAP EKITTEL A: "Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles", CELL MOL LIFE SCI, vol. 68, no. 16, 2011, pages 2667 - 2688, XP019929984, DOI: 10.1007/s00018-011-0689-3
GUTIERREZ-VAZQUEZ CVILLARROYA-BELTRI CMITTELBRUNN MSANCHEZ-MADRID F: "Transfer of extracellular vesicles during immune cell-cell interactions", IMMUNOL REV, vol. 251, no. 1, 2013, pages 125 - 142
COLOMBO MMOITA CVAN NIEL GKOWAL JVIGNERON JBENAROCH PMANEL NMOITA LFTHERY CRAPOSO G: "Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles", J CELL SCI, vol. 126, 2013, pages 5553 - 5565, XP055286174, DOI: 10.1242/jcs.128868
SETTE PJADWIN JADUSSUPT VBELLO NFBOUAMR F: "The ESCRT-associated protein Alix recruits the ubiquitin ligase Nedd4-1 to facilitate HIV-1 release through the LYPXnL L domain motif", J VIROL, vol. 84, no. 16, 2010, pages 8181 - 8192
TRAJKOVIC KHSU CCHIANTIA SRAJENDRAN LWENZEL DWIELAND FSCHWILLE PBRUGGER BSIMONS M: "Ceramide triggers budding of exosome vesicles into multivesicular endosomes", SCIENCE, vol. 319, no. 5867, 2008, pages 1244 - 1247
VAN NIEL GCHARRIN SSIMOES SROMAO MROCHIN LSAFTIG PMARKS MSRUBINSTEIN ERAPOSO G: "The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis", DEV CELL, vol. 21, no. 4, 2011, pages 708 - 721, XP028320002, DOI: 10.1016/j.devcel.2011.08.019
PALMA JYADDANAPUDI SCPIGATI LHAVENS MAJEONG SWEINER GAWEIMER KMSTERN BHASTINGS MLDUELLI DM: "MicroRNAs are exported from malignant cells in customized particles", NUCLEIC ACIDS RES, vol. 40, no. 18, 2012, pages 9125 - 9138, XP055292912, DOI: 10.1093/nar/gks656
ROBBINS PDMORELLI AE: "Regulation of immune responses by extracellular vesicles", NAT REV IMMUNOL, vol. 14, no. 3, 2014, pages 195 - 208, XP055324647, DOI: 10.1038/nri3622
ROYO FFALCON-PEREZ JM: "Liver extracellular vesicles in health and disease", J EXTRACELL VESICLES, vol. 1, 2012, pages 18825
WENDLER FSTAMP GWGIAMAS G: "Tumor-Stromal Cell Communication: Small Vesicles Signal Big Changes", TRENDS CANCER, vol. 2, no. 7, 2016, pages 326 - 329
BECKER ATHAKUR BKWEISS JMKIM HSPEINADO HLYDEN D: "Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis", CANCER CELL, vol. 30, no. 6, 2016, pages 836 - 848, XP029845231, DOI: 10.1016/j.ccell.2016.10.009
MULLER LMITSUHASHI MSIMMS PGOODING WEWHITESIDE TL: "Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets", SCI REP, vol. 6, 2016, pages 20254, XP055616614, DOI: 10.1038/srep20254
SEO NSHIRAKURA YTAHARA YMOMOSE FHARADA NIKEDA HAKIYOSHI KSHIKU H: "Activated CD8+ T cell extracellular vesicles prevent tumour progression by targeting of lesional mesenchymal cells", NAT COMMUN, vol. 9, no. 1, 2018, pages 435
ESTELLES ASPERINDE JROULON TAGUILAR BBONNER CLEPECQ JBDELCAYRE A: "Exosome nanovesicles displaying G protein-coupled receptors for drug discovery", INT J NANOMEDICINE, vol. 2, no. 4, 2007, pages 751 - 760
WUBBOLTS RLECKIE RSVEENHUIZEN PTSCHWARZMANN GMOBIUS WHOERNSCHEMEYER JSLOT JWGEUZE HJSTOORVOGEL W: "Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation", J BIOL CHEM, vol. 278, no. 13, 2003, pages 10963 - 10972, XP055196513, DOI: 10.1074/jbc.M207550200
DE GASSART AGEMINARD CFEVRIER BRAPOSO GVIDAL M: "Lipid raft-associated protein sorting in exosomes", BLOOD, vol. 102, no. 13, 2003, pages 4336 - 4344
MATSUMOTO ATAKAHASHI YNISHIKAWA MSANO KMORISHITA MCHAROENVIRIYAKUL CSAJI HTAKAKURA Y: "Role of Phosphatidylserine-Derived Negative Surface Charges in the Recognition and Uptake of Intravenously Injected B 16BL6-Derived Exosomes by Macrophages", J PHARM SCI, vol. 106, no. 1, 2017, pages 168 - 175
PAROLINI IFEDERICI CRAGGI CLUGINI LPALLESCHI SDE MILITO ACOSCIA CIESSI ELOGOZZI MMOLINARI A: "Microenvironmental pH is a key factor for exosome traffic in tumor cells", J BIOL CHEM, vol. 284, no. 49, 2009, pages 34211 - 34222, XP055201785, DOI: 10.1074/jbc.M109.041152
LOBB RJBECKER MWEN SWWONG CSWIEGMANS APLEIMGRUBER AMOLLER A: "Optimized exosome isolation protocol for cell culture supernatant and human plasma", J EXTRACELL VESICLES, vol. 4, 2015, pages 27031, XP055279331, DOI: 10.3402/jev.v4.27031
MOMEN-HERAVI FBALAJ LALIAN SMANTEL PYHALLECK AETRACHTENBERG AJSORIA CEOQUIN SBONEBREAK CMSARACOGLU E: "Current methods for the isolation of extracellular vesicles", BIOL CHEM, vol. 394, no. 10, 2013, pages 1253 - 1262, XP009191063
BARANYAI THERCZEG KONODI ZVOSZKA IMODOS KMARTON NNAGY GMAGER IWOOD MJEL ANDALOUSSI S: "Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods", PLOS ONE, vol. 10, no. 12, 2015, pages e0145686, XP055406112, DOI: 10.1371/journal.pone.0145686
WILLMS EJOHANSSON HJMAGER ILEE YBLOMBERG KESADIK MALAARG ASMITH CILEHTIO JEL ANDALOUSSI S: "Cells release subpopulations of exosomes with distinct molecular and biological properties", SCI REP, vol. 6, 2016, pages 22519
XU RGREENING DWZHU HJTAKAHASHI NSIMPSON RJ: "Extracellular vesicle isolation and characterization: toward clinical application", J CLIN INVEST, vol. 126, no. 4, 2016, pages 1152 - 1162, XP055500209, DOI: 10.1172/JCI81129
ZHANG ZWANG CLI TLIU ZLI L: "Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line-derived exosomes", ONCOL LETT, vol. 8, no. 4, 2014, pages 1701 - 1706
STRANSKA RGYSBRECHTS LWOUTERS JVERMEERSCH PBLOCH KDIERICKX DANDREI GSNOECK R: "Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma", J TRANSL MED, vol. 16, no. 1, 2018, pages 1, XP055590010, DOI: 10.1186/s12967-017-1374-6
NAKAI WYOSHIDA TDIEZ DMIYATAKE YNISHIBU TIMAWAKA NNARUSE KSADAMURA YHANAYAMA R: "A novel affinity-based method for the isolation of highly purified extracellular vesicles", SCI REP, vol. 6, 2016, pages 33935, XP055561243, DOI: 10.1038/srep33935
Attorney, Agent or Firm:
SHA Takuho (JP)
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