Title:
EXOSOME SECRETED BY LNCRNA GENE MODIFIED CELL AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/098101
Kind Code:
A1
Abstract:
Provided are an exosome secreted by an lncRNA gene modified cell and an application, relating to the field of cell biology. The exosome is secreted by an HEK-293T cell strain obtained by means of genetic engineering, the HEK-293T cell strain stably expresses lncENAF, and the nucleotide sequence of the lncENAF is as shown in SEQ ID NO: 1. The exosome can effectively inhibit generation of lipopolysaccharide-induced macrophage cytokines, is a potential cytokine inhibitor, and provides a new direction for cytokine storm and treatment of autoimmune diseases.
Inventors:
LI WEI (CN)
LIU LIN (CN)
HU XUDONG (CN)
LIU LIN (CN)
HU XUDONG (CN)
Application Number:
PCT/CN2022/106965
Publication Date:
June 08, 2023
Filing Date:
July 21, 2022
Export Citation:
Assignee:
UNIV WENZHOU MEDICAL (CN)
International Classes:
C12N15/113; A61K35/17; A61P7/00; A61P37/02; C12N5/10; C12N15/867
Foreign References:
| CN114058621A | 2022-02-18 | |||
| US20210024893A1 | 2021-01-28 | |||
| US20200407725A1 | 2020-12-31 | |||
| CN109837306A | 2019-06-04 | |||
| CN112538478A | 2021-03-23 |
Other References:
LI XUE, LEI YI, WU MIAO, LI NAN: "Regulation of Macrophage Activation and Polarization by HCC-Derived Exosomal lncRNA TUC339", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 19, no. 10, pages 2958, XP093070955, DOI: 10.3390/ijms19102958
CHEN QIAN, XIONG CHAOLIANG, JIA KUNYUN, JIN JING, LI ZIYANG, HUANG YAZHOU, LIU YEWEN, WANG LINGLING, LUO HAITAO, LI HAIYAN, MENG Q: "Hepatic transcriptome analysis from HFD-fed mice defines a long noncoding RNA regulating cellular cholesterol levels", JOURNAL OF LIPID RESEARCH, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC., US, vol. 60, no. 2, 1 February 2019 (2019-02-01), US , pages 341 - 352, XP093070956, ISSN: 0022-2275, DOI: 10.1194/jlr.M086215
GHAFOURI-FARD, S. ET AL.: "The impact of non-coding RNAs on macrophage polarization", BIOMEDICINE & PHARMACOTHERAPY, vol. 142, 27 August 2021 (2021-08-27), XP086785467, DOI: 10.1016/j.biopha.2021.112112
CHEN QIAN, XIONG CHAOLIANG, JIA KUNYUN, JIN JING, LI ZIYANG, HUANG YAZHOU, LIU YEWEN, WANG LINGLING, LUO HAITAO, LI HAIYAN, MENG Q: "Hepatic transcriptome analysis from HFD-fed mice defines a long noncoding RNA regulating cellular cholesterol levels", JOURNAL OF LIPID RESEARCH, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC., US, vol. 60, no. 2, 1 February 2019 (2019-02-01), US , pages 341 - 352, XP093070956, ISSN: 0022-2275, DOI: 10.1194/jlr.M086215
GHAFOURI-FARD, S. ET AL.: "The impact of non-coding RNAs on macrophage polarization", BIOMEDICINE & PHARMACOTHERAPY, vol. 142, 27 August 2021 (2021-08-27), XP086785467, DOI: 10.1016/j.biopha.2021.112112
Attorney, Agent or Firm:
BEIJING SHENGXUN IP AGENT LLC (CN)
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