Title:
EXPANSION CULTURE MEDIUM AND CULTURE METHOD FOR NEURAL CELLS
Document Type and Number:
WIPO Patent Application WO/2022/037570
Kind Code:
A1
Abstract:
A culture medium and a culture method for neural cells, particularly relating to a composition; the composition comprises an SMAD protein signaling pathway inhibitor, an SHH signaling pathway agonist, a Wnt signaling pathway agonist and a Myosin II ATPase inhibitor, and optionally further comprises an ROCK inhibitor; or the composition is composed of the SMAD protein signaling pathway inhibitor, the SHH signaling pathway agonist, the Wnt signaling pathway agonist, the Myosin II ATPase inhibitor and optionally the ROCK inhibitor. The same batch of neural cells, such as mesencephalic dopamine neural cell precursors, can be efficiently obtained by using the culture method and the culture medium designed on the basis of the composition.
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Inventors:
ZHOU QI (CN)
LI WEI (CN)
HAO JIE (CN)
WANG YUKAI (CN)
FENG LIN (CN)
WANG LIU (CN)
HU BAOYANG (CN)
SUN YUN (CN)
TIAN YAO (CN)
HE ZHENGQUAN (CN)
LI WEI (CN)
HAO JIE (CN)
WANG YUKAI (CN)
FENG LIN (CN)
WANG LIU (CN)
HU BAOYANG (CN)
SUN YUN (CN)
TIAN YAO (CN)
HE ZHENGQUAN (CN)
Application Number:
PCT/CN2021/112986
Publication Date:
February 24, 2022
Filing Date:
August 17, 2021
Export Citation:
Assignee:
INST ZOOLOGY CAS (CN)
BEIJING INST FOR STEM CELL AND REGENERATIVE MEDICINE (CN)
BEIJING INST FOR STEM CELL AND REGENERATIVE MEDICINE (CN)
International Classes:
A61K38/18; C12N5/0793; C12N5/0797
Foreign References:
| US20200172862A1 | 2020-06-04 | |||
| CN109880800A | 2019-06-14 | |||
| CN109844102A | 2019-06-04 | |||
| CN108531453A | 2018-09-14 | |||
| CN102940631A | 2013-02-27 | |||
| CN105658788A | 2016-06-08 | |||
| CN202010824749A | 2020-08-17 |
Other References:
MEDEIROS NELSON A., BURNETTE DYLAN T., FORSCHER PAUL: "Myosin II functions in actin-bundle turnover in neuronal growth cones", NATURE CELL BIOLOGY, vol. 8, no. 3, 1 March 2006 (2006-03-01), London, pages 216 - 226, XP055902350, ISSN: 1465-7392, DOI: 10.1038/ncb1367
BEJOY JULIE, SONG LIQING, LI YAN: "Wnt-YAP interactions in the neural fate of human pluripotent stem cells and the implications for neural organoid formation", ORGANOGENESIS, vol. 12, no. 1, 2 January 2016 (2016-01-02), US , pages 1 - 15, XP055902354, ISSN: 1547-6278, DOI: 10.1080/15476278.2016.1140290
GROOT SIMON C., SLIEDREGT KAREN, BENTHEM PETER PAUL G., RIVOLTA MARCELO N., HUISMAN MARGRIET A.: "Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology", THE ANATOMICAL RECORD, AMERICAN ASSOCIATION OF ANATOMISTS, vol. 303, no. 3, 1 March 2020 (2020-03-01), Hoboken, NJ : Wiley-Liss , pages 408 - 426, XP055902366, ISSN: 1932-8486, DOI: 10.1002/ar.24067
CAS , no. 483367-10-8
KIM ET AL.: "Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use", CELL STEM CELL, vol. 28, 2021, pages 343 - 355
TCHIEU ET AL.: "A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages", CELL STEM CELL, vol. 21, 2017, pages 399 - 410
BEJOY JULIE, SONG LIQING, LI YAN: "Wnt-YAP interactions in the neural fate of human pluripotent stem cells and the implications for neural organoid formation", ORGANOGENESIS, vol. 12, no. 1, 2 January 2016 (2016-01-02), US , pages 1 - 15, XP055902354, ISSN: 1547-6278, DOI: 10.1080/15476278.2016.1140290
GROOT SIMON C., SLIEDREGT KAREN, BENTHEM PETER PAUL G., RIVOLTA MARCELO N., HUISMAN MARGRIET A.: "Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology", THE ANATOMICAL RECORD, AMERICAN ASSOCIATION OF ANATOMISTS, vol. 303, no. 3, 1 March 2020 (2020-03-01), Hoboken, NJ : Wiley-Liss , pages 408 - 426, XP055902366, ISSN: 1932-8486, DOI: 10.1002/ar.24067
CAS , no. 483367-10-8
KIM ET AL.: "Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use", CELL STEM CELL, vol. 28, 2021, pages 343 - 355
TCHIEU ET AL.: "A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages", CELL STEM CELL, vol. 21, 2017, pages 399 - 410
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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