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Title:
EXPRESSION METHOD OF ACTIVE GOLDFISH TGF2 TRANSPOSON RECOMBINANT TRANSPOSASE PROTEIN
Document Type and Number:
WIPO Patent Application WO/2015/143930
Kind Code:
A1
Abstract:
An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1(DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22℃ in shaking of 150-200rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.

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Inventors:
ZOU SHUMING (CN)
JIANG XIAYUN (CN)
XU HAILI (CN)
HOU FEI (CN)
CHENG LUODAN (CN)
SHEN XIAODAN (CN)
DENG SHOUCHANG (CN)
Application Number:
PCT/CN2015/070017
Publication Date:
October 01, 2015
Filing Date:
January 04, 2015
Export Citation:
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Assignee:
UNIV SHANGHAI OCEAN (CN)
International Classes:
C12N1/21; C12N15/70; C12N9/12; C12N15/85
Domestic Patent References:
WO2001040477A12001-06-07
Foreign References:
CN103981204A2014-08-13
CN101962660A2011-02-02
CN101962659A2011-02-02
Other References:
JIANG, XIAYUN ET AL.: "Goldfish transposase Tgf2 presumably from recent horizontal transfer is active", THE FASEB JOURNAL, vol. 26, no. 7, 31 July 2012 (2012-07-31), pages 2743 - 2751, XP055227493, ISSN: 0892-6638
XU, HAILI ET AL.: "Prokaryotic Expression and Purification of Soluble Goldfish Tgf2 Transposase with Transposition Activity", MOL BIOTECHNOL, vol. 57, 5 November 2014 (2014-11-05), pages 94 - 100, XP035423697, ISSN: 1073-6085
Attorney, Agent or Firm:
SHANGHAI ZHUOYANG INTELLECTUAL PROPERTY AGENT FIRM (CN)
上海卓阳知识产权代理事务所（普通合伙） (CN)
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