EXPRESSION VECTOR FOR CELLULOSE PRODUCTION, TRANSFORMANT, AND CELLULOSE PRODUCTION METHOD

Title:

EXPRESSION VECTOR FOR CELLULOSE PRODUCTION, TRANSFORMANT, AND CELLULOSE PRODUCTION METHOD

Document Type and Number:

WIPO Patent Application WO/2014/104310

Kind Code:

A1

Abstract:

Provided is a method for readily and inexpensively producing cellulose. The production method uses a cellulose production vector having a promoter, a CesA gene, and a CesB gene, and having an upstream sequence of a 5'-side gene in a 5'-side gene-derived organism, between the promoter and the initiation codon for the 5'-side gene out of either the CesA gene or the CesB gene, whichever is further on the upstream side. A transformant having the cellulose production vector introduced thereto is cultivated, and cellulose is biosynthesized using the transformant, by causing the vector and a dgc gene to be co-expressed.

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Inventors:

IMAI TOMOYA (JP)
SUGIYAMA JUNJI (JP)

Application Number:

PCT/JP2013/085138

Publication Date:

July 03, 2014

Filing Date:

December 27, 2013

Export Citation:

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Assignee:

UNIV KYOTO (JP)

International Classes:

C12N15/09; C12N1/21; C12P19/04

Foreign References:

JPH04503456A

Other References:

SU , HSIANG-YEN ET AL.: "Increased cellulose production by heterologous expression of cellulose synthase genes in a filamentous heterocystous cyanobacterium with a modification in photosynthesis performance and growth ability.", BOTANICAL STUDIES, vol. 52, 2011, pages 265 - 275
TAL, RONY ET AL.: "Three cdg operons control cellular turnover of cyclic Di-GMP in Acetobacter xylinum: Genetic organization and occurrence of conserved domains in isoenzymes.", JOURNAL OF BACTERIOLOGY, vol. 180, 1998, pages 4416 - 4425
WONG, H.C. ET AL.: "GENETIC ORGANIZATION OF THE CELLULOSE SYNTHASE OPERON IN ACETOBACTER-XYLINUM.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 87, 1990, pages 8130 - 8134

Attorney, Agent or Firm:

TSUJIMARU Koichiro et al. (JP)
Koichiro Tsujimaru (JP)

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