Title:
EXPRESSION VECTOR FOR ESTABLISHING HYPER-PRODUCING CELLS, AND HYPER-PRODUCING CELLS
Document Type and Number:
WIPO Patent Application WO/2010/140387
Kind Code:
A1
Abstract:
Disclosed is an expression vector which is effective for efficiently establishing a transformed cell capable of expressing a gene for a protein of interest at a high level. Specifically disclosed is an expression vector which comprises: an expression cassette for a drug selection marker gene, which comprises an mRNA-destabilizing sequence; at least one gene expression stabilizing element; and an expression cassette for a gene for a protein of interest. Preferably, the mRNA-destabilizing sequence is derived from an AT-rich sequence occurring in a cytokine, an interleukin or a 3'-untranslated region of a protooncogene, and the gene expression stabilizing element is derived from the genome of a Chinese hamster.
Inventors:
YAMAZAKI TOMOMI (JP)
MASUDA KENJI (JP)
NISHII SHIGEAKI (JP)
KAWAKAMI BUNSEI (JP)
MASUDA KENJI (JP)
NISHII SHIGEAKI (JP)
KAWAKAMI BUNSEI (JP)
Application Number:
PCT/JP2010/003759
Publication Date:
December 09, 2010
Filing Date:
June 04, 2010
Export Citation:
Assignee:
TOYO BOSEKI (JP)
YAMAZAKI TOMOMI (JP)
MASUDA KENJI (JP)
NISHII SHIGEAKI (JP)
KAWAKAMI BUNSEI (JP)
YAMAZAKI TOMOMI (JP)
MASUDA KENJI (JP)
NISHII SHIGEAKI (JP)
KAWAKAMI BUNSEI (JP)
International Classes:
C12N15/09; C07K16/00; C12N5/10; C12P21/00
Domestic Patent References:
| WO2005024015A1 | 2005-03-17 | |||
| WO2001032901A1 | 2001-05-10 | |||
| WO2004050884A2 | 2004-06-17 |
Foreign References:
| JP2007503837A | 2007-03-01 | |||
| US5627033A | 1997-05-06 | |||
| JP2001037478A | 2001-02-13 |
Other References:
MILOUX B. ET AL: "Rapid isolation of highly productive recombinant Chinese Hamster Ovary cell lines", GENE, vol. 149, 1994, pages 341 - 344, XP023541780
BUTLER M.: "Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals", APPL. MICROBIOL. BIOTECHNOL., vol. 68, 2005, pages 283 - 291, XP019331930
KWAKS T. H. J. ET AL: "Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells", TRENDS BIOTECHNOL., vol. 24, no. 3, 2006, pages 137 - 142, XP027921749
HOUDEBINE L-M. ET AL: "Antibody manufacture in transgenic animals and comparisons with other systems", PHARMACEUTICAL BIOTECHNOL., vol. 13, 2002, pages 625 - 629, XP008164594
ALLISON D. S. ET AL: "Rapid Development of CHO Cell Lines for High-Level Production of Recombinant Antibodies", BIOPROCESSING J., vol. 2, no. 2, March 2003 (2003-03-01) - April 2003 (2003-04-01), pages 33 - 40, XP008165792
ZUBIAGA A. M. ET AL: "The Nonamer UUAUUUAUU Is the Key AU-Rich Sequence Motif That Mediates mRNA Degradation", MOL. CELL. BIOL., vol. 15, no. 4, 1995, pages 2219 - 2230, XP008164595
BAO L. ET AL: "CTCFBSDB: a CTCF-binding site database for characterization of vertebrate genomic insulators", NUCL. ACIDS RES., vol. 36, 2008, pages D83 - D87, XP055077388
KIM J-M. ET AL: "Improved recombinant gene expression in CHO cells using matrix attachment regions", J. BIOTECHNOL., vol. 107, 2004, pages 95 - 105, XP002458354
See also references of EP 2439269A4
NIWA H; YAMAMURA K; MIYAZAKI J., GENE, vol. 108, 1991, pages 193 - 200
PIKAART MJ; RECILLAS-TARGA F; FELSENFELD G., GENES DEV, vol. 12, 1998, pages 2852 - 2862
IZUMI M; GILBERT DM, J CELL BIOCHEM, vol. 76, 1999, pages 280 - 289
BAKHEET T; FREVEL M; WILLIAMS B. R. G; GREER W; KHABAR K. S. A., NUCLEIC ACIDS RESEARCH, vol. 29, 2001, pages 246 - 254
LAGNADO C. A; BROWN C. L; GOODALL G. J., MOLECULAR AND CELLULAR BIOLOGY, vol. 14, 1994, pages 7984 - 7995
ZUBIAGA A. M; BELASCO J. G; GREENBERG M. E., MOLECULAR AND CELLULAR BIOLOGY, vol. 15, 1995, pages 2219 - 2230
BLASCO MA, NAT REV GENET, vol. 8, 2007, pages 299 - 309
GONZALO S; JACO I; FRAGA MF; CHEN T; LI E; ESTELLER M; BLASCO MA, NAT CELL BIOL, vol. 8, 2006, pages 416 - 424
PERROD S; GASSER SM, CELL MOL LIFE SCI, vol. 60, 2003, pages 2303 - 2318
WAKIMOTO BT, CELL, vol. 93, 1998, pages 321 - 324
BAO L; ZHOU M; CUI Y, NUCLEIC ACIDS RESEARCH, vol. 36, 2008, pages D83 - D87
BUTLER M.: "Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals", APPL. MICROBIOL. BIOTECHNOL., vol. 68, 2005, pages 283 - 291, XP019331930
KWAKS T. H. J. ET AL: "Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells", TRENDS BIOTECHNOL., vol. 24, no. 3, 2006, pages 137 - 142, XP027921749
HOUDEBINE L-M. ET AL: "Antibody manufacture in transgenic animals and comparisons with other systems", PHARMACEUTICAL BIOTECHNOL., vol. 13, 2002, pages 625 - 629, XP008164594
ALLISON D. S. ET AL: "Rapid Development of CHO Cell Lines for High-Level Production of Recombinant Antibodies", BIOPROCESSING J., vol. 2, no. 2, March 2003 (2003-03-01) - April 2003 (2003-04-01), pages 33 - 40, XP008165792
ZUBIAGA A. M. ET AL: "The Nonamer UUAUUUAUU Is the Key AU-Rich Sequence Motif That Mediates mRNA Degradation", MOL. CELL. BIOL., vol. 15, no. 4, 1995, pages 2219 - 2230, XP008164595
BAO L. ET AL: "CTCFBSDB: a CTCF-binding site database for characterization of vertebrate genomic insulators", NUCL. ACIDS RES., vol. 36, 2008, pages D83 - D87, XP055077388
KIM J-M. ET AL: "Improved recombinant gene expression in CHO cells using matrix attachment regions", J. BIOTECHNOL., vol. 107, 2004, pages 95 - 105, XP002458354
See also references of EP 2439269A4
NIWA H; YAMAMURA K; MIYAZAKI J., GENE, vol. 108, 1991, pages 193 - 200
PIKAART MJ; RECILLAS-TARGA F; FELSENFELD G., GENES DEV, vol. 12, 1998, pages 2852 - 2862
IZUMI M; GILBERT DM, J CELL BIOCHEM, vol. 76, 1999, pages 280 - 289
BAKHEET T; FREVEL M; WILLIAMS B. R. G; GREER W; KHABAR K. S. A., NUCLEIC ACIDS RESEARCH, vol. 29, 2001, pages 246 - 254
LAGNADO C. A; BROWN C. L; GOODALL G. J., MOLECULAR AND CELLULAR BIOLOGY, vol. 14, 1994, pages 7984 - 7995
ZUBIAGA A. M; BELASCO J. G; GREENBERG M. E., MOLECULAR AND CELLULAR BIOLOGY, vol. 15, 1995, pages 2219 - 2230
BLASCO MA, NAT REV GENET, vol. 8, 2007, pages 299 - 309
GONZALO S; JACO I; FRAGA MF; CHEN T; LI E; ESTELLER M; BLASCO MA, NAT CELL BIOL, vol. 8, 2006, pages 416 - 424
PERROD S; GASSER SM, CELL MOL LIFE SCI, vol. 60, 2003, pages 2303 - 2318
WAKIMOTO BT, CELL, vol. 93, 1998, pages 321 - 324
BAO L; ZHOU M; CUI Y, NUCLEIC ACIDS RESEARCH, vol. 36, 2008, pages D83 - D87
Attorney, Agent or Firm:
KAZAHAYA, Nobuaki et al. (JP)
Nobuaki Kazahaya (JP)
Nobuaki Kazahaya (JP)
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