Title:
EXPRESSION VECTOR FOR USE IN METHANOTROPH
Document Type and Number:
WIPO Patent Application WO/2022/065916
Kind Code:
A1
Abstract:
Provided is a vector comprising: a first expression cassette comprising DmpR as a transcriptional control gene, a ribosome binding site, and a promoter operably linked to same; a second expression cassette comprising a sequence encoding a target polypeptide, a ribosome binding site, and a Po promoter operably linked to same. Also provided is a transformed methanotroph transformed by said vector.
Inventors:
LEE SEUNG GOO (KR)
LEE DAE HEE (KR)
LEE HYE WON (KR)
KIM SEONG KEUN (KR)
BAEK JI IN (KR)
KIM TAE HYUN (KR)
OH SO HYUNG (KR)
LEE DAE HEE (KR)
LEE HYE WON (KR)
KIM SEONG KEUN (KR)
BAEK JI IN (KR)
KIM TAE HYUN (KR)
OH SO HYUNG (KR)
Application Number:
PCT/KR2021/013043
Publication Date:
March 31, 2022
Filing Date:
September 24, 2021
Export Citation:
Assignee:
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
International Classes:
C12N15/74
Domestic Patent References:
| WO2015195972A1 | 2015-12-23 | |||
| WO2007022623A1 | 2007-03-01 |
Foreign References:
| KR20200062070A | 2020-06-03 | |||
| KR102112286B1 | 2020-05-19 |
Other References:
KWON KIL KOANG, YEOM SOO-JIN, CHOI SU-LIM, RHA EUGENE, LEE HYEWON, KIM HASEONG, LEE DAE-HEE, LEE SEUNG-GOO: "Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system", SCIENTIFIC REPORTS, vol. 10, no. 1, 1 December 2020 (2020-12-01), pages 6091, XP055913481, DOI: 10.1038/s41598-020-62892-1
KARLINALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
PEARSON, METHODS ENZYMOL., vol. 183, 1990, pages 63
ACS SYNTH BIOL., vol. 3, no. 3, 21 March 2014 (2014-03-21), pages 163 - 171
STOLYAR ET AL., MIKROBIOLOGIYA, vol. 64, 1995, pages 686 - 691
APPL ENVIRON MICROBIOL, vol. 81, no. 5, March 2015 (2015-03-01), pages 1775 - 1781
KARLINALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
PEARSON, METHODS ENZYMOL., vol. 183, 1990, pages 63
ACS SYNTH BIOL., vol. 3, no. 3, 21 March 2014 (2014-03-21), pages 163 - 171
STOLYAR ET AL., MIKROBIOLOGIYA, vol. 64, 1995, pages 686 - 691
APPL ENVIRON MICROBIOL, vol. 81, no. 5, March 2015 (2015-03-01), pages 1775 - 1781
Attorney, Agent or Firm:
PCR INTELLECTUAL PROPERTY LAW FIRM (KR)
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