Title:
EXTRACELLULAR HEME PRODUCTION METHOD USING METABOLICALLY ENGINEERED MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2019/117612
Kind Code:
A1
Abstract:
The present invention relates to a microorganism variome which is capable of extracellular heme generation, and more specifically, to a metabolically engineered microorganism variome which is capable of extracellular heme generation and a heme production method using same. Heme, a complex salt, is increasingly being used as a health food and food additive to treat porphyria, and the present invention has an advantageous effect of allowing high-yield, extracellular production of heme using a microorganism variome, rather than by existing methods of chemical synthesis, enzyme synthesis and the like.
Inventors:
LEE SANG YUP (KR)
ZHAO XIN RUI (KR)
CHOI KYEONG ROK (KR)
ZHAO XIN RUI (KR)
CHOI KYEONG ROK (KR)
Application Number:
PCT/KR2018/015748
Publication Date:
June 20, 2019
Filing Date:
December 12, 2018
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C12N15/67; C12P17/18
Foreign References:
| KR20140107025A | 2014-09-04 | |||
| KR20110070977A | 2011-06-27 | |||
| KR20080061801A | 2008-07-03 | |||
| EP0631631A1 | 1995-01-04 | |||
| US5681725A | 1997-10-28 | |||
| US20160340411A1 | 2016-11-24 |
Other References:
FEISSNER, R.E. ET AL: "Molecular Microbiology", ABC TRANSPORTER-MEDIATED RELEASE OF A HAEM CHAPERONE ALLOWS CYTOCHROME C BIOGENISIS, vol. 61, no. 1, 30 May 2006 (2006-05-30), pages 219 - 231, XP055612175, DOI: 10.1111/j.1365-2958.2006.05221.x
ZHANG, J.: "Optimization of the Heme Biosynthesis pathway for the production of 5-aminolevulinic acid in Escherichia Coli", SCIENTIFIC REPORTS, vol. 5, no. 8584, 26 February 2015 (2015-02-26), pages 1 - 7, XP055612203, DOI: 10.1038/srep08584
DAILEY, H.A.: "The Escherichia coli Protein YfeX Functions as a Porphyrinogen Oxidase, Not a Heme Dechelatase", MBIO, vol. 2, no. 6, 8 November 2011 (2011-11-08), pages e00248-11, XP055612208, DOI: 10.1128/mBio.00248-11
ZHAO, X.R.: "Metabolic engineering of escherichia Coli for secretory production of free Haem", NATURE CATALYSIS, vol. 1, no. 9, 1 September 2018 (2018-09-01), pages 720 - 728, XP055612213, DOI: 10.1038/s41929-018-0126-1
ANZALDI ET AL., INFECT.IMMUN., vol. 78, 2010, pages 4977 - 4989
FEISSNER ET AL., MOL. MICROBIOL., vol. 61, 2006, pages 219 - 231
LAYER G. ET AL., PROTEIN SCI., vol. 19, 2010, pages 1137 - 1161
DING ET AL., J. IND. MICROBIOL. BIOTECHNOL., vol. 44, 2017, pages 1127 - 1135
ZHANG ET AL., SCI. REP., vol. 5, 2015, pages 8584
KANG ET AL., WORLD J. MICROBIOL. BIOTECHNOL., vol. 33, 2017
KWON S. J. ET AL., APPL. ENVIRON. MICROBIOL., vol. 69, 2003, pages 4875 - 4883
KWON 0. H. ET AL., J. MICROBIOL. BIOTECHNOL., vol. 19, 2009, pages 604 - 609
LEE M. J. ET AL., J. MICROBIOL. BIOTECHNOL., vol. 23, 2013, pages 668 - 673
PRANAWIDJAJA S. ET AL., J. MICROBIOL. BIOTECHNOL., vol. 25, 2015, pages 880 - 886
STUDIER ET AL., J. MOL. BIOL., vol. 189, 1986, pages 113 - 130
BURNHAM, METHODS ENZYMOL., vol. 17A, 1970, pages 195 - 204
LI ET AL., FEMS MICROBIOL. LETT., vol. 350, 2014, pages 209 - 215
LEE ET AL., J. MICROBIOL. BIOTECHNOL., vol. 22, 2012, pages 1653 - 1658
BU ET AL., J. CHROMATOGR. B ANALYT. TECHNOL. BIOMED. LIFE SCI., vol. 783, 2003, pages 411 - 423
BURNHAM, METHODS ENZYMOL., vol. 17A, pages 195 - 204,1970
VUORISTO ET AL., AMB EXPRESS, vol. 5, 2015, pages 61
DATSENKO ET AL., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645
KIM ET AL., FEMS MICROBIOL. LETT, vol. 278, 2008, pages 78 - 85
WILD ET AL., GENE, vol. 223, 1998, pages 55 - 66
LETOFFE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 106, 2009, pages 11719 - 11724
DAILEY ET AL., MBIO, vol. 2, 2011, pages e00248 - 00211
TURLIN ET AL., MICROBIOLOGY OPEN, vol. 3, 2014, pages 849 - 859
KIM ET AL., FEMS MICROBIOL. LETT., vol. 278, 2008, pages 78 - 85
ANZALDI ET AL., INFECT. IMMUN., vol. 78, 2010, pages 4977 - 4989
SCHULZ ET AL., PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 6462 - 6467
ZHANG, J.: "Optimization of the Heme Biosynthesis pathway for the production of 5-aminolevulinic acid in Escherichia Coli", SCIENTIFIC REPORTS, vol. 5, no. 8584, 26 February 2015 (2015-02-26), pages 1 - 7, XP055612203, DOI: 10.1038/srep08584
DAILEY, H.A.: "The Escherichia coli Protein YfeX Functions as a Porphyrinogen Oxidase, Not a Heme Dechelatase", MBIO, vol. 2, no. 6, 8 November 2011 (2011-11-08), pages e00248-11, XP055612208, DOI: 10.1128/mBio.00248-11
ZHAO, X.R.: "Metabolic engineering of escherichia Coli for secretory production of free Haem", NATURE CATALYSIS, vol. 1, no. 9, 1 September 2018 (2018-09-01), pages 720 - 728, XP055612213, DOI: 10.1038/s41929-018-0126-1
ANZALDI ET AL., INFECT.IMMUN., vol. 78, 2010, pages 4977 - 4989
FEISSNER ET AL., MOL. MICROBIOL., vol. 61, 2006, pages 219 - 231
LAYER G. ET AL., PROTEIN SCI., vol. 19, 2010, pages 1137 - 1161
DING ET AL., J. IND. MICROBIOL. BIOTECHNOL., vol. 44, 2017, pages 1127 - 1135
ZHANG ET AL., SCI. REP., vol. 5, 2015, pages 8584
KANG ET AL., WORLD J. MICROBIOL. BIOTECHNOL., vol. 33, 2017
KWON S. J. ET AL., APPL. ENVIRON. MICROBIOL., vol. 69, 2003, pages 4875 - 4883
KWON 0. H. ET AL., J. MICROBIOL. BIOTECHNOL., vol. 19, 2009, pages 604 - 609
LEE M. J. ET AL., J. MICROBIOL. BIOTECHNOL., vol. 23, 2013, pages 668 - 673
PRANAWIDJAJA S. ET AL., J. MICROBIOL. BIOTECHNOL., vol. 25, 2015, pages 880 - 886
STUDIER ET AL., J. MOL. BIOL., vol. 189, 1986, pages 113 - 130
BURNHAM, METHODS ENZYMOL., vol. 17A, 1970, pages 195 - 204
LI ET AL., FEMS MICROBIOL. LETT., vol. 350, 2014, pages 209 - 215
LEE ET AL., J. MICROBIOL. BIOTECHNOL., vol. 22, 2012, pages 1653 - 1658
BU ET AL., J. CHROMATOGR. B ANALYT. TECHNOL. BIOMED. LIFE SCI., vol. 783, 2003, pages 411 - 423
BURNHAM, METHODS ENZYMOL., vol. 17A, pages 195 - 204,1970
VUORISTO ET AL., AMB EXPRESS, vol. 5, 2015, pages 61
DATSENKO ET AL., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645
KIM ET AL., FEMS MICROBIOL. LETT, vol. 278, 2008, pages 78 - 85
WILD ET AL., GENE, vol. 223, 1998, pages 55 - 66
LETOFFE ET AL., PROC. NATL. ACAD. SCI. USA, vol. 106, 2009, pages 11719 - 11724
DAILEY ET AL., MBIO, vol. 2, 2011, pages e00248 - 00211
TURLIN ET AL., MICROBIOLOGY OPEN, vol. 3, 2014, pages 849 - 859
KIM ET AL., FEMS MICROBIOL. LETT., vol. 278, 2008, pages 78 - 85
ANZALDI ET AL., INFECT. IMMUN., vol. 78, 2010, pages 4977 - 4989
SCHULZ ET AL., PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 6462 - 6467
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
Download PDF: