Title:
FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS
Document Type and Number:
WIPO Patent Application WO/2014/039702
Kind Code:
A3
Abstract:
Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
Inventors:
AINLEY WILLIAM MICHAEL (US)
WEBB STEVEN R (US)
SAMUEL PON (US)
GUSCHIN DMITRY Y (US)
MILLER JEFFREY C (US)
ZHANG LEI (US)
WEBB STEVEN R (US)
SAMUEL PON (US)
GUSCHIN DMITRY Y (US)
MILLER JEFFREY C (US)
ZHANG LEI (US)
Application Number:
PCT/US2013/058299
Publication Date:
July 24, 2014
Filing Date:
September 05, 2013
Export Citation:
Assignee:
DOW AGROSCIENCES LLC (US)
SANGAMO BIOSCIENCES INC (US)
SANGAMO BIOSCIENCES INC (US)
International Classes:
A01H5/00; C07H21/02; C07H21/04; C12N5/04; C12N15/82
Foreign References:
| US20110167521A1 | 2011-07-07 | |||
| US20110301073A1 | 2011-12-08 | |||
| US20070059795A1 | 2007-03-15 | |||
| US20130326645A1 | 2013-12-05 | |||
| US20110123509A1 | 2011-05-26 |
Other References:
DATABASE GENBANK [online] 25 January 2011 (2011-01-25), "Glycine max GmFAD2-1 a gene for mocrosomal omega-6 fatty acid desaturase", XP055242395, retrieved from NCBI Database accession no. AB188250
CURTIN ET AL.: "Targeted Mutagenesis of Duplicated Genes in Soybean with Zinc-Finger Nucleases1", PLANT PHYSIOLOGY, vol. 156, June 2011 (2011-06-01), XP055242424
CHI ET AL.: "Genome-Wide Analysis of Fatty Acid Desaturases in Soybean (Glycine max", PLANT MOL BIOL REP, vol. 29, 2011, XP019967112
CURTIN ET AL.: "Targeted Mutagenesis of Duplicated Genes in Soybean with Zinc-Finger Nucleases1", PLANT PHYSIOLOGY, vol. 156, June 2011 (2011-06-01), XP055242424
CHI ET AL.: "Genome-Wide Analysis of Fatty Acid Desaturases in Soybean (Glycine max", PLANT MOL BIOL REP, vol. 29, 2011, XP019967112
Attorney, Agent or Firm:
PASTERNAK, Dahna S. (1900 Embarcadero Rd.Suite 21, Palo Alto California, US)
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