Title:
FEN ENDONUCLEASES
Document Type and Number:
WIPO Patent Application WO2002070755
Kind Code:
A3
Abstract:
The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
Inventors:
LYAMICHEV VICTOR I (US)
KAISER MICHAEL W (US)
LYAMICHEVA NATASHA (US)
KAISER MICHAEL W (US)
LYAMICHEVA NATASHA (US)
Application Number:
PCT/US2001/044953
Publication Date:
June 19, 2003
Filing Date:
November 15, 2001
Export Citation:
Assignee:
THIRD WAVE TECH INC (US)
LYAMICHEV VICTOR I (US)
KAISER MICHAEL W (US)
LYAMICHEVA NATASHA (US)
LYAMICHEV VICTOR I (US)
KAISER MICHAEL W (US)
LYAMICHEVA NATASHA (US)
International Classes:
C12N15/09; C12N9/12; C12N9/16; C12N9/22; C12Q1/02; C12Q1/34; C12Q1/68; A61K38/00; (IPC1-7): C12Q1/68; C12N9/22
Domestic Patent References:
| WO1998023774A1 | 1998-06-04 |
Other References:
HOSFILED D.J. ET AL.,: "newly discovered archaebacterial flap endonucleases show a structure-specific mechanism for DNA substrate binding and catalysis resembling human flap endonuclease-1", J. BIOL. CHEMISTRY, vol. 273, no. 42, 1998, pages 27154 - 27161, XP002215815
KIMURA S ET AL: "PLANT HOMOLOGUE OF FLAP ENDONUCLEASE-1: MOLECULAR CLONING, CHARACTERIZATION, AND EVIDENCE OF EXPRESSION IN MERISTEMATIC TISSUES", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 42, 2000, pages 415 - 427, XP000886911, ISSN: 0167-4412
BINGHUI S ET AL: "Flap endonuclease homologs in archaebacteria exist as independent proteins", TIBS TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER PUBLICATION, CAMBRIDGE, EN, vol. 23, no. 5, 1 May 1998 (1998-05-01), pages 171 - 173, XP004118516, ISSN: 0968-0004
KAISER M W ET AL: "A COMPARISON OF EUBACTERIAL AND ARCHAEAL STRUCTURE-SPECIFIC 5'-EXONUCLEASES", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 30, no. 274, 23 July 1999 (1999-07-23), pages 21387 - 21394, XP001028490, ISSN: 0021-9258
LYAMICHEV V ET AL: "Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 3, March 1999 (1999-03-01), pages 292 - 296, XP002121762, ISSN: 1087-0156
KIMURA S ET AL: "PLANT HOMOLOGUE OF FLAP ENDONUCLEASE-1: MOLECULAR CLONING, CHARACTERIZATION, AND EVIDENCE OF EXPRESSION IN MERISTEMATIC TISSUES", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 42, 2000, pages 415 - 427, XP000886911, ISSN: 0167-4412
BINGHUI S ET AL: "Flap endonuclease homologs in archaebacteria exist as independent proteins", TIBS TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER PUBLICATION, CAMBRIDGE, EN, vol. 23, no. 5, 1 May 1998 (1998-05-01), pages 171 - 173, XP004118516, ISSN: 0968-0004
KAISER M W ET AL: "A COMPARISON OF EUBACTERIAL AND ARCHAEAL STRUCTURE-SPECIFIC 5'-EXONUCLEASES", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 30, no. 274, 23 July 1999 (1999-07-23), pages 21387 - 21394, XP001028490, ISSN: 0021-9258
LYAMICHEV V ET AL: "Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 3, March 1999 (1999-03-01), pages 292 - 296, XP002121762, ISSN: 1087-0156
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