Title:
FERMENTATION TECHNIQUE TO IMPROVE PRODUCTION LEVEL OF RECOMBINANT HUMAN COLLAGEN
Document Type and Number:
WIPO Patent Application WO/2018/014453
Kind Code:
A1
Abstract:
The present invention provides a fermentation technique to improve the production level of a recombinant human collagen. First inoculating a sterilized fermentation culture medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hours before adding methanol to induce the expression, and adding sodium pyruvate into the culture medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L.
Inventors:
YANG SHULIN (CN)
DU ERFENG (CN)
HUANG JIANMIN (CN)
GAO LIHU (CN)
ZHAO JIANFENG (CN)
TAO HAI (CN)
FENG LIPING (CN)
ZHOU AIMEI (CN)
JI LE (CN)
DU ERFENG (CN)
HUANG JIANMIN (CN)
GAO LIHU (CN)
ZHAO JIANFENG (CN)
TAO HAI (CN)
FENG LIPING (CN)
ZHOU AIMEI (CN)
JI LE (CN)
Application Number:
PCT/CN2016/102435
Publication Date:
January 25, 2018
Filing Date:
October 18, 2016
Export Citation:
Assignee:
JIANGSU JLAND BIOTECH CO LTD (CN)
International Classes:
C07K14/78; C12P21/02; C12R1/84
Domestic Patent References:
| WO2014170460A2 | 2014-10-23 |
Foreign References:
| CN103725623A | 2014-04-16 |
Other References:
H. YIN ET AL.: "Intracellular Expression and Purification of the Canstatin-N Protein in Pichia Pastoris", GENE, vol. 504, no. 1, 2 May 2015 (2015-05-02), pages 122 - 126, XP 055452700
LI, BAOKU ET AL.: "Study on the Culture Conditions of L-lactic Acid Bacteria", LIQUOR-MAKING SCIENCE & TECHNOLOGY, 31 December 2007 (2007-12-31), pages 51 - 57, XP009511442
See also references of EP 3473726A4
LI, BAOKU ET AL.: "Study on the Culture Conditions of L-lactic Acid Bacteria", LIQUOR-MAKING SCIENCE & TECHNOLOGY, 31 December 2007 (2007-12-31), pages 51 - 57, XP009511442
See also references of EP 3473726A4
Attorney, Agent or Firm:
JIANGSU SHENGDIAN LAW FIRM (CN)
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