Title:
FERMENTATIVE PRODUCTION OF ALCOHOLS
Document Type and Number:
WIPO Patent Application WO/2013/102084
Kind Code:
A3
Abstract:
The invention relates to the fields of industrial microbiology and alcohol production. The invention also relates to the development of a microorganism capable of producing fermentation products via an engineered pathway, and uses of the microorganism. A process for producing butanol comprising the following steps: (a) providing a butanologen, (b) contacting the butanologen with one or more carbon substrates under conditions wherein butanol is produced at an effective yield; (c) collecting the butanologen; (d) recovering butanol at a concentration of at least about 6 g/L; (e) contacting the collected butanologen of (c) with one or more carbon substrates under conditions wherein butanol is produced at an effective yield and wherein the effective yield is at least about 90% of the effective yield of (b); (f) repeating steps (c)-(e); and, optionally exposing the collected butanologen of (c) to conditions of pH less than or equal to about 2.0 for at least about 1 hour in the presence of at least about 0.3% butanol. The invention also relates to the methods to improve cell viability and productivity and the use of recycling and acid washing to increase the yield of fermentation products.
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Inventors:
BHADRA BHASKAR (IN)
BHALLA RITU (IN)
KRUCKEBERG ARTHUR LEO (US)
NAGARAJAN VASANTHA (US)
PATNAIK RANJAN (US)
SUH WONCHUL (US)
BHALLA RITU (IN)
KRUCKEBERG ARTHUR LEO (US)
NAGARAJAN VASANTHA (US)
PATNAIK RANJAN (US)
SUH WONCHUL (US)
Application Number:
PCT/US2012/072079
Publication Date:
October 24, 2013
Filing Date:
December 28, 2012
Export Citation:
Assignee:
BUTAMAX TM ADVANCED BIOFUELS (US)
International Classes:
C12P7/16
Domestic Patent References:
| WO2011159962A1 | 2011-12-22 | |||
| WO2011097711A1 | 2011-08-18 |
Other References:
XIAOPING YANG ET AL: "Enhanced acetone-butanol fermentation using repeated fed-batch operation coupled with cell recycle by membrane and simultaneous removal of inhibitory products by adsorption", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 47, no. 4, 20 August 1995 (1995-08-20), pages 444 - 450, XP002505045, ISSN: 0006-3592, DOI: 10.1002/BIT.260470405
STEEN ERIC J ET AL: "Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, NL, vol. 7, no. 1, 3 December 2008 (2008-12-03), pages 36, XP021049875, ISSN: 1475-2859, DOI: 10.1186/1475-2859-7-36
CHEN XIAO ET AL: "Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism.", BIOTECHNOLOGY FOR BIOFUELS 2011, vol. 4, 21, 28 July 2011 (2011-07-28), pages 12PP, XP002696693, ISSN: 1754-6834, DOI: 10.1186/1754-6834-4-21
EZEJI T C ET AL: "Acetone butanol ethanol (ABE) production from concentrated substrate: Reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 63, no. 6, 1 February 2004 (2004-02-01), pages 653 - 658, XP002512560, ISSN: 0175-7598, [retrieved on 20030809], DOI: 10.1007/S00253-003-1400-X
ATSUMI S ET AL: "Metabolic engineering of Escherichia coli for 1-butanol production", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 10, no. 6, 1 November 2008 (2008-11-01), pages 305 - 311, XP025800632, ISSN: 1096-7176, [retrieved on 20081101], DOI: 10.1016/J.YMBEN.2007.08.003
STEEN ERIC J ET AL: "Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, NL, vol. 7, no. 1, 3 December 2008 (2008-12-03), pages 36, XP021049875, ISSN: 1475-2859, DOI: 10.1186/1475-2859-7-36
CHEN XIAO ET AL: "Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism.", BIOTECHNOLOGY FOR BIOFUELS 2011, vol. 4, 21, 28 July 2011 (2011-07-28), pages 12PP, XP002696693, ISSN: 1754-6834, DOI: 10.1186/1754-6834-4-21
EZEJI T C ET AL: "Acetone butanol ethanol (ABE) production from concentrated substrate: Reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 63, no. 6, 1 February 2004 (2004-02-01), pages 653 - 658, XP002512560, ISSN: 0175-7598, [retrieved on 20030809], DOI: 10.1007/S00253-003-1400-X
ATSUMI S ET AL: "Metabolic engineering of Escherichia coli for 1-butanol production", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 10, no. 6, 1 November 2008 (2008-11-01), pages 305 - 311, XP025800632, ISSN: 1096-7176, [retrieved on 20081101], DOI: 10.1016/J.YMBEN.2007.08.003
Attorney, Agent or Firm:
PELLEGRINO, Susan, M. (DuPont Experimental StationBuilding 35, Wilmington Delaware, US)
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