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Title:
FERMENTED HERBAL HEALTH DRINK FROM PLANT $i(ANDROGRAPHIS)
Document Type and Number:
WIPO Patent Application WO/2003/062367
Kind Code:
A1
Abstract:
The present invention relates to a fermented herbal health drink, comprising extract from plant Andrographis, extract from barley, wheat, and/or fruit and ethanol concentration ranging between 3 to 13% in the total health drink and optionally extract from plant Tinospora and other additives, said drink not containing hops and still having characteristic taste of a beer with no adverse effect and contributing to several medicinal properties comprising anti-oxidant, nervine relaxant, choleretic, cardio-tonic, diuretic, digestive and immunomodulant, and a method of producing the herbal health drink, preferably a method of introducing bitterness into said health drink and using the same as a beer or to produce beer with said medicinal properties.

Inventors:
PUSHPANGADAN PALPU (IN)
MEHROTRA SHANTA (IN)
RAWAT AJAY KUMAR SINGH (IN)
KHATOON SAYYADA (IN)
SRIVASTAVA SHARAD KUMAR (IN)
RASTOGI SUBHA (IN)
CHAUBEY MANJOOSHA (IN)
AGNIHOTRI ADARSH KUMAR (IN)
Application Number:
PCT/IB2002/000389
Publication Date:
July 31, 2003
Filing Date:
January 25, 2002
Export Citation:
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Assignee:
COUNCIL SCIENT IND RES (IN)
PUSHPANGADAN PALPU (IN)
MEHROTRA SHANTA (IN)
RAWAT AJAY KUMAR SINGH (IN)
KHATOON SAYYADA (IN)
SRIVASTAVA SHARAD KUMAR (IN)
RASTOGI SUBHA (IN)
CHAUBEY MANJOOSHA (IN)
AGNIHOTRI ADARSH KUMAR (IN)
International Classes:
A23L1/30; A23L2/52; A61K36/00; A61K36/19; A61K36/22; A61K36/47; A61K36/59; A61K36/61; A61K36/899; A61K36/8998; C12C5/02; C12C12/00; C12G3/02; C12G3/04; C12G3/06; (IPC1-7): C12C12/00; C12G3/06; A61K35/78
Foreign References:
US6136316A2000-10-24
Other References:
DATABASE WPI Section Ch Week 199805, Derwent World Patents Index; Class B04, AN 1998-043001, XP002213534
DAMU A G ET AL: "Two acylated flavone glucosides from Andrographis serpyllifolia", PHYTOCHEMISTRY, PERGAMON PRESS, GB, vol. 52, no. 1, September 1999 (1999-09-01), pages 147 - 151, XP004290981, ISSN: 0031-9422
CHEUNG H Y ET AL: "Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER SCIENCE, NL, vol. 930, no. 1-2, 28 September 2001 (2001-09-28), pages 171 - 176, XP004306385, ISSN: 0021-9673
PAL D C: "SOME PLANTS IN TRADITIONAL AND HOMOEOPATHIC SYSTEMS OF MEDICINE IN INDIA", JOURNAL OF ECONOMIC AND TAXONOMIC BOTANY, SCIENTIFIC PUBLISHERS, JODHPUR,, IN, vol. 23, no. 1, 15 September 1999 (1999-09-15), pages 79 - 83, XP000952694, ISSN: 0250-9768
Attorney, Agent or Firm:
GABRIEL, DEVADOSS, CALAB, KUMARAN & SAGAR (C6 Lane Off Central Avenue Sainik Farms, New Delhi 2, IN)
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Claims:
Claims
1. A fermented fruit based herbal health drink useful as an antioxidant, cardiotonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13% in the total herbal health drink, optionally extract from plant Tinospora and other additives.
2. The health drink as claimed in claim 1 wherein, other additives are selected from a group comprising coloring agents, sugar, carbonated water and preservatives.
3. The health drink as claimed in claim 1 wherein, fermentation enhancers are selected from a group comprising grains of wheat and barley.
4. The health drink as claimed in claim 1 wherein, fruits are selected from a group comprising Mangifera indica, Phyllanthus emblica, Psidium guajava, and Citrus spp.
5. The health drink as claimed in claim 1 wherein, said health drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the extracts of plant Tinospora or Andrographis alone.
6. The health drink as claimed in claim 1 has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.
7. The health drink as claimed in claim 1 wherein, plant material is crushed in water in ratio ranging between 1: 1 to 1: 1.5.
8. The health drink as claimed in claim 1 wherein, the fermentation enhancer is mixed with the fruit extract at the concentration ranging between 10 to 20%.
9. The health drink as claimed in claim 1 can be freshly prepared throughout the year.
10. The health drink as claimed in claim 1 wherein, plant extract is mixed with fruit extract at concentration ranging between 0.5 to 2%.
11. The health drink as claimed in claim 1 wherein, sugar is added to fruit extract in the ratio ranging between 1: 3 and 2: 5.
12. The health drink as claimed in claim 1 wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.
13. : The health drink as claimed in claim 1 has bitterness value ranging between 175 to 210.
14. The health drink as claimed in claim 1 wherein, all the vegetative part of the said plants contribute to bitterness.
15. The health drink as claimed in claim 1 has alcohol content ranging between 3 to 13 %.
16. The health drink claimed in claim 1 wherein said drink has aroma, refreshing property, an ability to quench thirst, provides instant energy, fight fatigue, safe, health protective.
17. The health drink claimed in claim 1 wherein, medicinal properties of said plant extract is not affected by the presence of other constituents of the said drink.
18. A method of producing fermented fruit based herbal health drink useful as an antioxidant, cardiotonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13% in total health drink, optionally extract from plant Tinospora and other additives, said method comprising: (i) obtaining aqueous extract from plant Andrographis, (ii) concentrating the aqueous extract, (iii) lyophilizing the concentrated extract, (iv) adding sugar to fruit extract, (v) obtaining fruit base material, (vi) autoclaving the said base material, (vii) mixing fermentation enhancer and lyophilized extract with base material, (viii) adding mixture of step (vii) with said inoculum for fermentation, in aseptic conditions, (ix) fermenting mixture of step (viii) for 4 to 10 days, (x) filtering the fermented material, (xi) pasteurizing the filtrate, (xii) adding carbonated water to pasteurized filtrate, (xiii) obtaining fermented fruit based health drink, and (xiv) adding preservatives and coloring agents to drink of step (xiii).
19. A method as claimed in claim 18 wherein, other additives are selected from a group comprising coloring agents, sugar, carbonated water and preservatives. coloring agents, and preservatives.
20. A method as claimed in claim 18 wherein, fermentation enhancers are selected from a group comprising grains of wheat and barley.
21. A method as claimed in claim 18 wherein, fruits are selected from a group comprising Mangifera indica, Phyllanthus emblica, Psidium guajava, and Citrus spp.
22. A method as claimed in claim 18 wherein, said health drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the extract of plant Tinospora or Andrographis alone.
23. A method as claimed in claim 18 wherein, has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.
24. A method of producing health drink as claimed in claim 18 wherein, plant parts are crushed in water in ratio ranging between 1: 1 to 1: 1.5.
25. A method as claimed in claim 18 wherein, the fermentation enhancer is mixed with the fruit base material at the concentration ranging between 10 to 20%.
26. A method as claimed in claim 18 wherein, said drink can be freshly prepared throughout the year.
27. A method as claimed in claim 18 wherein, plant extract is mixed with the fruit base material at the concentration ranging between 0.5 to 2%.
28. A method as claimed in claim 18 wherein, sugar is added to fruit filtrate in the ratio ranging between 1: 3 and 2 : 5.
29. A method as claimed in claim 18 wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.
30. A method as claimed in claim 18 wherein, said drink has bitterness value ranging between 175 to 210.
31. A method as claimed in claim 18 wherein, all the vegetative part of the said plants contributes to bitterness.
32. A method as claimed in claim 18 wherein, said drink has alcohol content ranging between 2 to 8 %.
33. A method as claimed in claim 18 wherein, said drink has aroma, refreshing property, an ability to quench thirst, provides instant energy, fight fatigue, safe, and health protective.
34. A method as claimed in claim 18 wherein, medicinal properties of said plant extract is not affected by the presence of other constituents of the said drink.
35. Fermented health drink as herbal beer without hops, having characteristic bitter taste of beer, useful as an antioxidant, cardiotonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13% in the total health drink, optionally extract from plant Tinospora and other additives.
36. The health drink as herbal beer without hops as claimed in claim 35 wherein, other additives are selected from a group comprising coloring agents, sugar, carbonated water, and preservatives.
37. The health drink as herbal beer without hops as claimed in claim 35 wherein, fruits are selected from a group comprising Mangifera indica, Phyllanthus emblica, Psidium guajava, and Citrus spp.
38. The health drink as herbal beer without hops as claimed in claim 35 has none of the disadvantages of conventional beer.
39. The health drink as herbal beer without hops as claimed in claim 35 has bitterness value ranging between 175 to 210.
40. The health drink as herbal beer without hops as claimed in claim 35 wherein, all the vegetative part of the said plants contributes to bitterness.
41. The health drink as herbal beer without hops as claimed in claim 35 has alcohol content ranging between 3 to 13 %.
42. The health drink as herbal beer without hops as claimed in claim 35 wherein, said drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the extract of plant Tinospora or Andrographis alone.
43. The health drink as herbal beer without hops as claimed in claim 35 has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.
44. The health drink as claimed in claim 35 wherein, plant material is crushed in water in ratio ranging between 1: 1 to 1: 1.5.
45. The health drink as herbal beer without hops as claimed in claim 35 can be freshly prepared throughout the year.
46. The health drink as herbal beer without hops as claimed in claim 35 wherein, plant extract is mixed with the fruit extract at concentration ranging between 0.5 to 2%.
47. The health drink as herbal beer without hops as claimed in claim 35 wherein, sugar is added to fruit extract in the ratio ranging between 1: 3 and 2: 5.
48. The health drink as herbal beer without hops as claimed in claim 35 wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.
49. The health drink as herbal beer without hops as claimed in claim 35 has aroma, refreshing property, an ability to quench thirst, provides instant energy, helps fight fatigue, safe, and health protective.
50. A method of introducing bitterness into drink and using the said drink as herbal beer, said beer not containing hops and still retaining its characteristic bitter taste and also contributing to medicinal properties, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13% in the total herbal health drink, optionally extract from plant Tinospora and other additives, said method comprising: (i) obtaining aqueous extract from said plant, (ii) concentrating the aqueous extract, (iii) lyophilizing the concentrated extract, (iv) crushing fruit pulp to get fruit extract, (v) mixing lyophilized extract with sugar and fruit extract, (vi) adding mixture of step (v) with said inoculum for fermentation, in aseptic conditions, (vii) fermenting mixture of step (vi) for 4 to 10 days, (viii) filtering the fermented material, (ix) pasteurizing the filtrate, (x) obtaining stock herbal drink, (xi) adding carbonated water to stock herbal drink, and (xii) obtaining herbal beer.
51. A method as claimed in claim 50 wherein, said herbal beer has none of the disadvantages of conventional beer.
52. A method as claimed in claim 50 wherein, additives are selected from a group comprising coloring agents, sugar, carbonated water and preservatives.
53. A method as claimed in claim 50 wherein, fruits are selected from a group comprising Mangifera indica, Phyllanthus emblica, Psidium guajava, and Citrus spp.
54. A method as claimed in claim 50 wherein, said health drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the plant extract Tinospora or Andrographis alone.
55. A method as claimed in claim 50 wherein, said drink is an antioxidant, cardiotonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant.
56. A method as claimed in claim 50 wherein, extract of plants Andrographis and Tinospora in the ratio ranging between 25 : 1 to 1: 150.
57. A method as claimed in claim 50 wherein, plant parts are crushed in water in ratio ranging between 1: 1 to 1: 1.5.
58. A method as claimed in claim 50 wherein, said drink can be freshly prepared throughout the year.
59. A method as claimed in claim 50 wherein, concentration of plant extract ranging between 0.5 to 2%.
60. A method as claimed in claim 50 wherein, sugar is added to the mixture in the ratio ranging between 1: 3 and 2 : 5.
61. A method as claimed in claim 50 wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.
62. A method as claimed in claim 50 wherein, said drink has bitterness value ranging between 175 to 210.
63. A method as claimed in claim 50 wherein, all the vegetative part of the said plants contributes to bitterness.
64. A method as claimed in claim 50 wherein, said drink has alcohol content ranging between 3 to 13 %.
65. A method as claimed in claim 50 wherein, said drink has aroma, has refreshing property, an ability to quench thirst, provides instant energy, fight fatigue, safe, and health protective.
66. A method as claimed in claim 50 wherein, medicinal properties of said plant extract is not affected by the presence of other constituents in the said drink.
67. A fermented herbal health drink useful as an antioxidant, cardiotonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from barley and/or wheat, and ethanol ranging between 3 to 13% in the total herbal health drink, optionally extract from plant Tinospora and other additives.
68. The health drink as claimed in claim 67 wherein, other additives are selected from a group comprising coloring agents, sugar, carbonated water and preservatives.
69. The health drink as claimed in claim 67 wherein, said health drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the extracts of plant Tinospora or Andrographis alone.
70. The health drink as claimed in claim 67 has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.
71. The health drink as claimed in claim 67 wherein, plant material is crushed in water in ratio ranging between 1: 1 to 1: 1.5.
72. The health drink as claimed in claim 67 can be freshly prepared throughout the year.
73. The health drink as claimed in claim 67 wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.
74. The health drink as claimed in claim 67 has bitterness value ranging between 175 to 210.
75. The health drink as claimed in claim 67 wherein, all the vegetative part of the said plants contribute to bitterness.
76. The health drink as claimed in claim 67 has alcohol content ranging between 3 to 13 %.
77. The health drink claimed in claim 67 wherein said drink has aroma, refreshing property, an ability to quench thirst, provides instant energy, fight fatigue, safe, health protective.
78. The health drink claimed in claim 67 wherein, medicinal properties of said plant extract is not affected by the presence of other constituents of the said drink.
79. A method of producing fermented herbal health drink useful as an antioxidant, cardio tonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from barley and/or wheat, and ethanol ranging between 3 to 13% in total health drink, optionally extract from plant Tinospora and other additives, said method comprising: (i) obtaining aqueous extract from plant Ahdrographis, (ii) concentrating the aqueous extract, (iii) lyophilizing the concentrated extract, (iv) adding sugar to wheat and/or barley extract to form base material, (v) autoclaving the said base material, (vi) mixing fermentation enhancer and lyophilized extract with base material, (vii) adding mixture of step (vii) with said inoculum for fermentation, in aseptic conditions, (viii) fermenting mixture of step (viii) for 4 to 10 days, (ix) filtering the fermented material, (x) pasteurizing the filtrate, (xi) adding carbonated water to pasteurized filtrate, (xii) obtaining fermented health drink, and (xiii) adding preservatives and coloring agents to drink of step (xiii).
80. A method as claimed in claim 79 wherein, other additives are selected from a group comprising coloring agents, sugar, carbonated water and preservatives.
81. A method as claimed in claim 79 wherein, said health drink obtained by combination of extract from plants Ahdrographis and Tinospora is superior to the one obtained from the extract of plant Tinospora or Andrographis alone.
82. A method as claimed in claim 79 wherein, has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.
83. A method as claimed in claim 79 wherein, plant parts are crushed in water in ratio ranging between 1: 1 to 1 : 1.5.
84. A method as claimed in claim 79 wherein, said drink can be freshly prepared throughout the year.
85. A method as claimed in claim 79 wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.
86. A method as claimed in claim 79 wherein, said drink has bitterness value ranging between 175 to 210.
87. A method as claimed in claim 79 wherein, all the vegetative part of the said plants contributes to bitterness.
88. A method as claimed in claim 79 wherein, said drink has alcohol content ranging between 2 to 8 %.
89. A method as claimed in claim 79 wherein, said drink has aroma, refreshing property, an ability to quench thirst, provides instant energy, fight fatigue, safe, and health protective.
90. A method as claimed in claim 79 wherein, medicinal properties of said plant extract is not affected by the presence of other constituents of the said drink.
Description:
FERMENTED HERBAL HEALTH DRINK FROM PLANT ANDROGRAPHIS Technical Field The present invention relates to a fermented herbal health drink, comprising extract from plant Andrographis, extract from barley, wheat, and/or fruit and ethanol concentration ranging between 3 to 13% in the total health drink and optionally extract from plant Tinospora and other additives, said drink not containing hops and still having characteristic taste of a beer with no adverse effect and contributing to several medicinal properties comprising anti-oxidant, nervine relaxant, cholerectic, cardio-tonic, diurectic, digestive and immunomodulant, and a method of producing the herbal health drink, preferably a method of introducing bitterness into said health drink and using the same as a beer or to produce beer with said medicinal properties.

Background Art Beer is an alcoholic beverage made from malted grains, hops, yeast and water. Fruit, herbs and spices are also used for flavors.

In the distant past, the term beer and'ale'meant different things-'Ale'referred to drink without using hops while'beer'with hops. Since virtually all commercial products now use hops, obtained from the female strobiles of Himulus lupulus, a temperate growing plant, and cultivated in England, Germany, Belgium, France, Russia and California.

In India it can be grown only in high attitude of Kashmir and Himalayas. The inventors undertook a critical study of wildly growing Indian medicinal plants, which are bitter in taste and can be a substitute of hops in alcoholic beverages, preferably in beer.

Reference is made to website (http://www. botanical. com/botanical/mgmh/h/hops-32. html) wherein, recipes for herb beers like Dandelion, Nettle, Meadow, Sweet, Yarrow hop and bitters are given. Hop Bitters are mentioned as appetizer and the Nettle Beer is often given as a remedy for gouty and rheumatic pains as shown in a website <BR> <BR> (http : //www. botanical. com/botanical/mgmh/n/nettle. 03. html\<BR> Another reference is made to website http : //www. foodnavigator. com-news letter, dated 2.1. 2001 wherein Riva Brewery of Dentegem, Belgium brewed fruity beers with cherries and strawberries.

Yet another reference is made to the Soma Products Co. at www. somaherbal. com wherein, 'Soma herbal'-a non-alcoholic herbal beer containing only 70 calories per bottle have been marketed by Soma Products Co. (Cherry Hill-NJ). SOMA is a precise blend of herbs.

Scullcap, Passion Flower, St. John's Wort and Kava Kava and the herbal formulation is designed to make the consumer feel relaxed and satisfied with a healthy herbal high or "huzz".

Still another reference is made to the website (http ://www. winxwired. com/4point3/bp43. html) wherein the flavored beers are developed from oysters, carrots, peppermint, strawberry, rasberry, cherry and other fruits. Another 'medicinal herbal beer'has been developed by bog myrtle, yellow and marsh rosemary in place of hops.

Reference is made to Stephen Beaumont (1999) who tested a new beer flavored with fresh oranges prepared by Craftsman, Brewing Company, California. <BR> <BR> <P>Reference is made to website http : //www. sallys-place. com/beverages/ber wherein Sara Doersam, a beer judge, described the history of beer brewing. Wheat beer, Vintage beer, Oktoberfest, real Ale, Red beer, Sake, Stout beer, Belgian beers etc. and her ten favorite beers. Almost all beers contain either hops for bitterness or hop flavor.

Another reference is made to the website http://www. foodnavigator. com/ingredients2 wherein a concentrated beer is described. It is prepared by spray drying beer with maltodrestrin, which removes water and alcohol, concentrating the flavor of beer in a free flowing powder.

Reference is made to a patent number JP2000060486 wherein, a food and drink showing hypercholesteremia improving action and anti-inflammatory action contain Tinospora tuberculata powder or its extract in a ratio of 1-7%. The other properties like antioxidant, immunoenhancing, anti-fatigue, antiaging and hepatoprotective are not found in this drink and it is also not reported as a alternative source of hops for bitterness in beer.

Reference is made to website http//www. mediket2000. com/altemativemedi/ayurvedtherapy wherein, Tinospora cordifolia (guduchi) is mentioned as an alternative medicine of ayurveda. It is reported as a good tonic to help build up new tissues, increases longevity, helps in rejuvenation of body, and also helps relieves stress and strain.

Reference is made to a website www. ayurvedhealthcare. com, wherein tinospora cordfolia is mentioned as antioxidant, immunomodulator, hypoglycemic, analgesic, tonic, antipyretic, anti-arthritic, anti-inflammatory, anti-allergic and anti-stress, are also reported as beneficial in AIDS.

Reference is also made to website http://www. realbeer. com/spencer/ wherein a detailed description of beer prepared by hops is given. <BR> <BR> <P>Reference is made of (http : //www. geocities. com/heart-land/prairie/3490/herbal-rem. html) wherein the medicinal properties of beer like sedative, stomach soothing, diuretic, appetite, stimulating and as a relieving agent in nervine spasm. The properties like antioxidant,

immuno-enhancing, anti-aging, anti-fatigue and hepatoprotective and are not found in hops.

Diet or food therapy is emerging as the latest trend in health care programme. It is now believed that there will be more dieticians than physicians will in the present century, as many diseases can be prevented if the right kind of diet is prescribed. Consumption of the right kind of food articles and drinks suited to the climate, age and constitution. Nature of the individuals are getting greater scientific, with high stress on health protection. The rich and traditional diet practice is prevalent among various communities with the regional variations, which are now found to be health protective/promotive. Over 1000 different kinds of alcoholic drinks, soft drinks, beverages and medicinal drinks are traditionally consumed in India. But unfortunately, with the introduction of various exotic drinks, many of the local drinks, which are mostly plant based, are fast disappearing and some of them are totally forgotten.

Disclosure of the present invention The main object of the present invention is to develop herbal health drink.

Another object of the present invention is to develop fruit based herbal health drink.

Yet another object of the present invention is to develop herbal health drink using plant Andrographis.

Still another object of the present invention is to develop herbal health drink using plant Andrographis in combination with plant Tinospora.

Still another object of the present invention is to develop herbal health drink with medicinal properties.

Still another object of the present invention is to develop herbal health drink with taste of a beer.

Still another object of the present invention is to develop method to produce herbal health drink having medicinal properties.

Still another object of the present invention is to develop method to introduce bitterness into herbal health drink.

Still another object of the present invention is to prepare herbal beer with Andrographis replacing hops for bitterness in the same.

Summary of the present invention The present invention relates to a fermented herbal health drink, comprising extract from plant Andrographis, extract from barley, wheat, and/or fruit and ethanol concentration ranging between 3 to 13% in the total health drink and optionally extract from plant

Tinospora and other additives, said drink not containing hops and still having characteristic taste of a beer with no adverse effect and contributing to several medicinal properties comprising anti-oxidant, nervine relaxant, cholerectic, cardio-tonic, diurectic, digestive and immunomodulant, and a method of producing the herbal health drink, preferably a method of introducing bitterness into said health drink and using the same as a beer or to produce beer with said medicinal properties.

Detailed description of the present invention Accordingly the present invention relates to a fermented fruit based herbal health drink useful as an antioxidant, cardio-tonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13% in the total herbal health drink, optionally extract from plant Tinospora and other additives.

In an embodiment of the present invention additives are selected from a group comprising coloring agents, sugar, carbonated water, and preservatives.

In another embodiment of the present invention wherein, fermentation enhancers are selected from a group comprising grains of wheat and barley.

In yet another embodiment of the present invention wherein, fruits are selected from a group comprising Mangifera indica, Phyllanthus emblica, Psidium guajava, and Citrus spp.

In still another embodiment of the present invention wherein, said health drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the plant Tinospora or Andrographis alone.

In still another embodiment of the present invention, the plant extract is obtained in aqueous medium or organic medium. The natures of medium do not affect the properties of the said drink.

In still another embodiment of the present invention has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.

In still another embodiment of the present invention wherein, fermentation enhancer is mixed with the fruit extract at the concentration ranging between 10 to 20%.

In still another embodiment of the present invention can be freshly prepared throughout the year.

In still another embodiment of the present invention wherein, plant extract is mixed with the fruit base material at the concentration ranging between 0.5 to 2%.

In still another embodiment of the present invention wherein, sugar is added to fruit extract in the ratio ranging between 1: 3 and 2: 5.

In still another embodiment of the present invention wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.

In still another embodiment of the present invention has bitterness value ranging between 175 to 210.

In still another embodiment of the present invention wherein, all the vegetative part of the said plants contributes to bitterness.

In still another embodiment of the present invention has alcohol content ranging between 2 to 8%.

In still another embodiment of the present invention has aroma.

In still another embodiment of the present invention has refreshing property.

In still another embodiment of the present invention has an ability to quench thirst.

In still another embodiment of the present invention provides instant energy.

In still another embodiment of the present invention fight fatigue.

In still another embodiment of the present invention the drink is a safe health protective and preventive drink.

In still another embodiment of the present invention wherein, medicinal properties of said plant extract is not affected by the presence of other constituents of the said drink.

In an embodiment of the present invention a method of producing a fermented fruit based herbal health drink useful as an antioxidant, cardio-tonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13% in the total herbal health drink, optionally extract from plant Tinospora and other additives.

In another embodiment of the present invention obtaining aqueous extract from said plant, In yet another embodiment of the present invention concentrating the aqueous extract, In still another embodiment of the present invention lyophilizing the concentrated extract, In still another embodiment of the present invention crushing said fruit extract, In still another embodiment of the present invention adding sugar to crushed fruit extract, In still another embodiment of the present invention obtaining fruit base material, In still another embodiment of the present invention autoclaving the said base material, In still another embodiment of the present invention mixing fermentation enhancer and lyophilized extract with base material,

In still another embodiment of the present invention adding mixture with said inoculum for fermentation, in aseptic conditions, In still another embodiment of the present invention fermenting mixture for 4 to 10 days, In still another embodiment of the present invention filtering the fermented material, In still another embodiment of the present invention pasteurizing the filtrate, In still another embodiment of the present invention obtaining stock herbal drink, In still another embodiment of the present invention adding carbonated water to stock herbal drink, In still another embodiment of the present invention obtaining fruit based fermented health drink, and In still another embodiment of the present invention adding preservatives and coloring agents to drink.

In still another embodiment of the present invention wherein, additives are selected from a group comprising coloring agents, and preservatives.

In still another embodiment of the present invention wherein, fermentation enhancers are selected from a group comprising grains of wheat and barley.

In still another embodiment of the present invention wherein, said health drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the plant Tinospora or Andrographis alone.

In still another embodiment of the present invention wherein, has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.

In still another embodiment of the present invention wherein, plant parts are crushed in water in ratio ranging between 1: 1 to 1: 1.5 to prepare aqueous extract.

In still another embodiment of the present invention, the aqueous extract can be prepared in both hot and cold water.

In still another embodiment of the present invention wherein, fermentation enhancer is mixed with the fruit base material at the concentration ranging between 10 to 20%.

In still another embodiment of the present invention wherein, said drink can be freshly prepared throughout the year.

In still another embodiment of the present invention wherein, plant extract is mixed with the fruit base material at the concentration ranging between 0.5 to 2%.

In still another embodiment of the present invention wherein, sugar is added to fruit filtrate in the ratio ranging between 1: 3 and 2: 5.

In still another embodiment of the present invention wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.

In still another embodiment of the present invention wherein, said drink has bitterness value ranging between 175 to 210.

In still another embodiment of the present invention wherein, all the vegetative part of the said plants contributes to bitterness.

In still another embodiment of the present invention wherein, said drink has alcohol content ranging between 2 to 8 %.

In still another embodiment of the present invention wherein, said drink has aroma.

In still another embodiment of the present invention wherein, said drink claimed has refreshing property.

In still another embodiment of the present invention wherein, said drink claimed has an ability to quench thirst.

In still another embodiment of the present invention wherein, said drink provides instant energy.

In still another embodiment of the present invention wherein, said drink fight fatigue.

In still another embodiment of the present invention wherein, said drink is a safe health protective and preventive drink.

In still another embodiment of the present invention wherein, medicinal properties of said plant extract is not affected by the presence of other constituents of the said drink.

In an embodiment of the present invention, fermented health drink as herbal beer without hops, having characteristic bitter taste of beer, useful as an antioxidant, cardio-tonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13% in the total health drink, optionally extract from plant Tinospora and other additives.

In still another embodiment of the present invention, for preparing herbal beer additives are selected from a group comprising coloring agents, sugar, carbonated water, and preservatives.

In still another embodiment of the present invention for preparing herbal beer has bitterness value ranging between 175 to 210.

In still another embodiment of the present invention wherein, for preparing herbal beer all the vegetative part of the said plants contributes to bitterness.

In still another embodiment of the present invention for preparing herbal beer has alcohol content ranging between 3 to 13 %.

In still another embodiment of the present invention for preparing herbal beer wherein, said

drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the plant Tinospora or Andrographis alone.

In still another embodiment of the present invention for preparing herbal beer has extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.

In still another embodiment of the present invention for preparing herbal beer can be freshly prepared throughout the year.

In still another embodiment of the present invention for preparing herbal beer wherein, plant extract is mixed with the fruit base material at concentration ranging between 0.5 to 2%.

In still another embodiment of the present invention for preparing herbal beer wherein, sugar is added to fruit base material in the ratio ranging between 1: 3 and 2: 5.

In still another embodiment of the present invention for preparing herbal beer wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.

In still another embodiment of the present invention herbal beer has aroma.

In still another embodiment of the present invention herbal beer has refreshing property.

In still another embodiment of the present invention herbal beer has an ability to quench thirst.

In still another embodiment of the present invention herbal beer provides instant energy.

In still another embodiment of the present invention herbal beer helps fight fatigue.

In still another embodiment of the present invention herbal beer is a safe health protective and preventive drink.

In an embodiment of the present invention a method of introducing bitterness into drink and using the said drink as herbal beer, said beer not containing hops and still retaining its characteristic bitter taste and also contributing to medicinal properties, comprising extract from plant Andrographis with concentration ranging between 0.5 to 10%, extract from fruit, and ethanol ranging between 3 to 13%, optionally extract from plant Tinospora and other additives.

In another embodiment of the present invention obtaining aqueous extract from said plant, In yet another embodiment of the present invention concentrating the aqueous extract, In still another embodiment of the present invention lyophilizing the concentrated extract, In still another embodiment of the present invention crushing optional fruit extract, In still another embodiment of the present invention mixing lyophilized extract with sugar, In still another embodiment of the present invention adding mixture of step (v) with said inoculum for fermentation, in aseptic conditions,

In still another embodiment of the present invention fermenting mixture of step (vi) for 4 to 10 days, In still another embodiment of the present invention filtering the fermented material, In still another embodiment of the present invention pasteurizing the filtrate, In still another embodiment of the present invention obtaining stock herbal drink, In still another embodiment of the present invention adding carbonated water to stock herbal drink, and In still another embodiment of the present invention obtaining herbal beer.

In still another embodiment of the present invention additives are selected from a group comprising coloring agents, and preservatives.

In still another embodiment of the present invention wherein, said health drink obtained by combination of extract from plants Andrographis and Tinospora is superior to the one obtained from the plant Tinospora or Andrographis alone.

In still another embodiment of the present invention wherein, said drink is an antioxidant, cardio- tonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant.

In still another embodiment of the present invention wherein, extract of plants Andrographis and Tinospora in the ratio ranging between 25: 1 to 1: 150.

A method of producing health drink as claimed in claim 62 wherein, plant parts are crushed in water in ratio ranging between 1: 1 to 1: 1.5 to prepare aqueous extract.

In still another embodiment of the present invention, the said aqueous extract can be prepared by adding both cold and hot water in the plant material.

In still another embodiment of the present invention wherein, said drink can be freshly prepared throughout the year.

In still another embodiment of the present invention wherein, concentration of plant extract ranging between 0.5 to 2%.

In still another embodiment of the present invention wherein, sugar is added to the mixture in the ratio ranging between 1: 3 and 2: 5.

In still another embodiment of the present invention wherein, carbonated water is added to stock herbal drink in the ratio ranging between 1: 3 to 2: 5 to get herbal drink.

In still another embodiment of the present invention wherein, said drink has bitterness value ranging between 175 to 210.

In still another embodiment of the present invention wherein, all the vegetative part of the said plants contributes to bitterness.

In still another embodiment of the present invention wherein, said drink has alcohol content ranging between 3 to 13 %.

In still another embodiment of the present invention wherein, said drink has aroma.

In still another embodiment of the present invention wherein, said drink claimed has refreshing property.

In still another embodiment of the present invention wherein, said drink claimed has an ability to quench thirst.

In still another embodiment of the present invention wherein, said drink provides instant energy.

In still another embodiment of the present invention wherein, said drink fight fatigue.

In still another embodiment of the present invention wherein, said drink is a safe health protective and preventive drink.

In still another embodiment of the present invention wherein, medicinal properties of said plant extract is not affected by the presence of other constituents of the said drink.

In still another embodiment of the present invention, advantage of using said plants in health drink is that, it is available throughout the year in tropical zones too and the vegetative parts like leaf and stem have all the aforesaid medicinal properties.

In still another embodiment of the present invention, the vegetative parts of selected plants may give bitterness to the drink.

In still another embodiment of the present invention, the part of medicinal herbs is selected from leaf, stem, root and whole plant.

In still another embodiment of the present invention, the selected plant parts are crushed in water in the ratio ranging between 1: 1 to 1: 1.5, to get the plant juices.

In still another embodiment of the present invention, a process for the preparation of plant extract the three layers of the Aqueous plant extract are separated out.

In still another embodiment of the present invention, the aqueous layer is concentrated by using rotavapor at a temperature of 50°C.

In still another embodiment of the present invention, all the three layers are lyophilized to make the powder form of the same.

In still another embodiment of the present invention, the raw fruits for preparing fruit based health drink are pealed off.

In still another embodiment of the present invention, the said pealed fruits and medicinal herb are crushed separately at room temperature.

In still another embodiment of the present invention, the crushed medicinal plant is mixed with the water in a ratio in the range of 1: 3 to 2: 7.

In still another embodiment of the present invention, the crushed fruits and aqueous mixture of medicinal herbs are filtered through muslin cloth separately.

In still another embodiment of the present invention, bitter powder from the plant extract is prepared.

In still another embodiment of the present invention, the base material from the crushed fruit is prepared.

In still another embodiment of the present invention, base material is autoclaved at 15 lbs. pressure for 15 to 20 minutes.

In still another embodiment of the present invention, said mixture is kept for fermentation for 4 to 10 days, in laboratory and 30-48 hrs. in frequenters.

In still another embodiment of the present invention, the said fermented material is filtered through muslin cloth.

In still another embodiment of the present invention, said filtrate is centrifuged at 10000 rpm for 15-30 minutes.

In still another embodiment of the present invention, the said filtrate as stock herbal drink is pasteurized.

In still another embodiment of the present invention, the said cereal grains are crushed and boiled or powdered.

In still another embodiment of the present invention, the combination of plant extract from Andrographis and Tinospora has better anti-oxidant, immunomodulation, hepatoprotectant, diuretic, nervine relaxant, anti-fatigue, digestive, cholerectic properties.

In an embodiment of the present invention, herbal beer from the combination of plants Andrographis and Tinospora is of superior quality as compared to the one formed from the plant Andrographis alone.

In still another embodiment of the present invention, herbal beer with none of the disadvantages of the conventional beer is developed.

In still another embodiment of the present invention, herbal beer with characteristic bitter taste of a conventional beer is developed.

In still another embodiment of the present invention beer with medicinal properties, such as antioxidant, anti-fatigue, health protective, immunomodulatory, hepatoprotective, diuretic etc, is developed.

In still another embodiment of the present invention, raw mango is optionally used as a base material to develop fruit based herbal beer with all the desired characteristics of the plant Andrographis alone and in combination with Tinospora.

In still another embodiment of the present invention, the combination of plant extract from Ahdrographis and Tinospora has better anti-oxidant, immunomodulation, hepatoprotectant, diuretic, nervine relaxant, anti-fatigue, digestive, cholerectic properties.

In an embodiment of the present invention a fermented herbal health drink useful as an antioxidant, cardio-tonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant Afzdrographis with concentration ranging between 0.5 to 10%, extract from barley and/or wheat, and ethanol ranging between 3 to 13% in the total herbal health drink, optionally extract from plant Tinospora and other additives.

In another embodiment of the present invention a method of producing fermented herbal health drink useful as an antioxidant, cardio-tonic, diuretic, digestive, choleretic, nervine relaxant and immunomodulant, comprising extract from plant AradrogTraphis with concentration ranging between 0.5 to 10%, extract from barley and/or wheat, and ethanol ranging between 3 to 13% in total health drink, optionally extract from plant Tinospora and other additives.

In yet another embodiment of the present invention obtaining aqueous extract from plant Andrographis, In still another embodiment of the present invention concentrating the aqueous extract, In still another embodiment of the present invention lyophilizing the concentrated extract, In still another embodiment of the present invention adding sugar to wheat and/or barley extract to form base material, In still another embodiment of the present invention autoclaving the said base material, In still another embodiment of the present invention mixing fermentation enhancer and lyophilized extract with base material, In still another embodiment of the present invention adding mixture of step (vii) with said inoculum for fermentation, in aseptic conditions, In still another embodiment of the present invention fermenting mixture of step (viii) for 4 to 10 days, In still another embodiment of the present invention filtering the fermented material, In still another embodiment of the present invention pasteurizing the filtrate, In still another embodiment of the present invention adding carbonated water to pasteurized filtrate, In still another embodiment of the present invention obtaining fermented health drink, and In still another embodiment of the present invention adding preservatives and coloring agents to drink of step (xiii).

In still another embodiment of the present invention, a local survey of about 100 people suggest that the herbal drink of instant application is liked by the bear drinkers and they feel that the herbal drink prepared by inventors can replace the conventional beer. The said herbal drink is liked for its special bitter tinge and medicinal properties. The data-sheet of the said survey is enclosed herewith.

In still another embodiment of the present invention, the bitterness value of different samples of a drink was determined according to the method given in"Quality control methods for medicinal plants", a WHO (1998) publication. However, it was determined using chloroquine phosphate as the positive control instead of quinine hydrochloride.

Bitterness value of herbal drink in different batches ranged from 180 to 207.

Herbal drink batches Bitterness value 1-With Afzdrographis extract (barley base) 180.44 2-With Tinospora Extract + Andrographis extract (Mango base) 193.33 3-With Aradrographis extract only (Mango base) 206.22 4-With Tinospora extract (Barely base) 200.62 It was also determined in market beer available commercially.

Haywards 5000 189.03 In still another embodiment of the present invention the alcohol percentage indifferent samples of the drinks was determined according to the specific gravity method for alcohol (by volume) in beer given in the Official Methods of Analysis AOAC (fourth edition, 1984). In ranged from 2% to 8% in the same batches of herbal drink.

Herbal drink batches Alcohol percentage 1-With Andrographis extract (barley base) 2.75% 2-With Tinospora Extract + Afzdographis extract (Mango base) 6.45% 3-WithAndrographis extract only (Mango base) 5.71% 4-With Tinospora extract (Barely base) 2.48% It was also determined in market beer available commercially.

Haywards 6.68% 01QBAA650 019113750

The medicinal plants used in the fermented herbal drink are reported to have antioxidant, immunomodulator, anti-fatigue and hepatoprotective properties. No commercial beer has the aforesaid properties.

The following examples are provided to illustrate the invention in accordance with the principles of this invention, but are not to be construed as limiting the invention in any way Example 1 Mix 2. 5kg of barley malt, 750g of sugar with 2.5 liters of distilled water. Add 40g of lower layer of Andrographis spp. extract 25g of yeast and keep the material in a fermenter for 48 hrs at a temperature of 30°C. After complete fermentation, filter the mixture and pasteurize, centrifuge at 12,000 rpm for 15 minutes. Prepare the drink by adding 25ml of soda water to 15ml of the prepared beer. Add preservatives in the concentration ranging between 2 to 10%. Add tartrazine as coloring agent.

Example 2 Mix 2. 0kg of crushed and boiled wheat, 0. 5kg of sugar with 2 liters of distilled water. Add 25g of lower layer, 5g of middle layer of Andrographis aqueous extract and 25g of yeast and keep the material in a fermenter for 48hrs at a temperature of 30°C. After complete fermentation, filter the mixture and pasteurize. Centrifuge at 12,000 rpm for 15 minutes.

Prepare the drink by adding 25ml of soda water to 15ml of the prepared beer. Add preservatives in the ratio ranging between 1 to 13%. Add caramel as coloring agent.

Example 3 Mix 10kg of barley malt extract, 4. 0kg of sugar, 150g of Tinospora aqueous extract 2g of Andrographis aqueous extract and 70 ml of Sacrornysis strain (inoculum) with 15 liters of distill water. keep the material in a fermenter for 40 hrs at a temperature of 30°C. After complete fermentation, filter the mixture and pasteurize, then centrifuge at 12,000 rpm for 30 minutes. Prepare the drink by adding 25 ml of soda water to 15 ml of the prepared beer.

Add preservatives in the ratio ranging between 0.5 to 7%. Add S02 as a preservative.

Example 4 Mix 2.4kg of Mango pulp with 2. 0kg of sugar. Add 2.5 liters of distilled water then autoclave the mixture. Add 40g of lower layer of andrographis spp. extract, 25g of yeast (inoculum) and 0. 5kg of barley. Keep the material in a fermenter for 36 to 98 hrs. After fermentation, filter the mixture and pasteurize, centrifuge at 12,000 rpm for 15 minutes.

Prepare the drink by adding 25 ml of soda water to 15 ml of the golden coloured stock solution and a pinch of salt. Add preservatives in the ratio ranging between 1 to 15%.

Example 5 Mix 2.4kg of Mango pulp with 2. 5kg of sugar and 2.5 liters of distill water. Autoclave the mixture. Add 175g of aqueous extract of Tinospora to 25g of dries aqueous extract of Andrographis spp. Sacromyces strain and 0. 5kg of crushed wheat, keep the mixture for 5 days for fermentation at a temperature of 30°C. Then the beer is filtered and pasteurized.

To get the transparent golden color the beer is centrifuged at 10,000 rpm for 30 minutes.

To prepared a drink, 10ml of stock herbal drink is further diluted with 25 ml of soda water.

Add preservatives. Add tert-butyl hydroquinine as an anti-oxidant.

Example 6 Mix 20 litres of Mango juice with 6.4kg of sugar. add 20 liters of distilled water and autoclave the mixture. Add 2g of dried extract of Andrographis, 100ml of Sacromyces inoculums and 1. 6kg of barley. Keep the material in a fermenter for 36 hrs. After completing fermentation, filter the mixture and pasteurize, then centrifuge at 12,000 rpm for 15 minutes. Prepare the drink by adding 25ml of soda water to 15 ml of the golden coloured stock solution and a pinch of salt. Add preservatives in the ratio ranging between 4 to 20%.

Example 7 Mix 2 litres of Playllanthus emblica fruit juice with lkg of sugar then add 2 liters of distill water, autoclave the mixture for 30mins at 15 pound. Add 20g each of middle and lower layers of Andrographis extract and 100g of yeast. The mixture kept for fermentation for 5 days, filter and centrifuge at 12, 000rpm for 45mins and then autoclaved. 10ml of this stock herbal beer is further diluted with 25ml of soda water and ready for use. And if desired add preservatives.

Example 8 Mix 3 liters of water melon juice, 1.5 liters of pomegranate juice and 405g of sugar then add 25g of aqueous extract of Andrographis and autoclave the mixture. Then add 20ml of Sacromyces strain. Filter the material after 4 days complete fermentation, centrifuge at 12,000 rpm for 30 minutes and then autoclave for 15 minutes. Add 20ml of said water to 10ml of the above stock herbal beer and add a small pinch of salt. Add preservatives. DATE NAME AND ADDRESS TASTE REMARKS 6/7/2000 KRISHNA BEHARI Very Good It is a very good TRIVEDI (AIR, Lucknow) drink for all. 6/7/2000 MEENU KHANNA, P. Ex. Good taste with a Bitter tinge should (AIR, Lucknow) specific bitter be removed tinge 6/7/2000 Dr. ANITA GAUTAM Seems to be a Very-Very good replacement Interesting! for beer drunkers CONGRATS! 7/7/2000 Dr. R. K. GUPTA Very good (i) However, it requires certain modication like its content of alcohol should be around 4% (ii) sweetness should be slightly reduced (may be reduced after enhancing the alcohol content). (iii) Blending of soda is not proper and 12/7/2000 S. K. TANDON Good If flavoured will be accepted by masses. It's quite like beer. 11/08/2000 RAMESH V. BHATT Agreeable but Drink has a strong (Deputy Director Sr. Gr. ) needs flavor of NATIONAL INSTITUTE OF improvement fermentation. Can NUTRIENTS, Hyderabad-be marketed to 500 009 specific segments of population for eg.: Health conscious group. 11/08/2000 ANUP P. S. KADE I like the taste It can be a good 131/8A, Chandigarh-100018 specifically the substitute for water taste it leaves on aerated drinks plus the tongue after it improves health. one has consumed the drink. 11/08/2000 D. K. Upreti, Scientist Good taste but too Alcoholic contents sweet. Needs should be increased. improvement 11/08/2000 AJAY KUMAR, JS (CSIR) Good This drink has the potential to make us the leader in Global Market and restore that India is rightful place assumed by others. 11/08/2000 RADHA K. MAHESHWARI, Excellent I have been very (US) impressed and looking forward to collaborate with you and your Institutions. 11/08./2000 Dr. D. PRAKASH, Scientist Outstanding With excellent taste (NBRI) flavor and refreshing 12/08/2000 Maharani B. K. Devi Unusual I wish all success to Ujjayanta Palace, Agartala, our country's new Tripura venture. 224/63 11/08/2000 KBK D. B. Burman (Good Maharaja of Tripura) 11/08/2000 Dr. R. P. AGARWAL, 1/53 Excellent Should come as a Okhla Mang. Ltd. market product (Industrialist) 22/08/2000 PRAVIN TRIPATHI, 7A-Excellent Will sell like crazy CSIR in the market 22/08/2000 MANJARI MISHRA Unusual An interesting venture 24/09/2000 DHAVAL BUCH, (Hindustan Excellent An absolutely Lever Ltd. ) fascinating experience. Potential must be explored. 24/09/2000 VIPUL CHAWLA (Hindustal Outstanding Potential to Lever Ltd. ) explore. Consumer possibilities must be explored. Very unusual product feel. 25/09/2000 ANUPAM SHARMA, City Good News, 23/24, IT Colony, Aaish bag Waterwork Road, Lucknow 27/09/2000 G. K. SRIVASTAVA, Botany Bitter It is just like beer in Department, Allahabad taste, very near to it, University, gives pleasing effect. 27/09/2000 Dr. M. BALARAMAN Bitter NAIR, Chief consultant Pathologist, Drug Research Centre, Trivandrum, Kerala 02/11/2000 Dr. MATHIAS CORCUT, Good interesting Herbal beer: Munich, Germany new taste, sour colour standardization? Herbal cola: Ifeel it a bit unbalanced. More sugar? More herbs? 07/12/2000 FIEJES STAMBOKES, Very good Resembles apple Budapest, Hungary. wine or wheat beer. Really refreshing. 07/12/2000 Dr. VIRENDRA NATH, Excellent Excellent health NBRI Lucknow drink, Just like wine. Congratulations ! 19/12/2000 P. K. GUPTA, CCSK, Meerut Beer is excellent Excellent work, needs extension activity. 19/12/2000 S. S. BIR, Punjab University, Excellent taste of Excellent work is Patiala true drink of plant being done by the origin Institute. Best wishes for more progress.-S. S. Bir 19/12/2000 D. K. CHAUHAN, Botany Excellent The taste of herbal Dept. Allahabad University, beer, is unique and Allahabad tasty - D. K. Chauhan 05/01/2001 Dr. Arvind & Dr. Adkar & Excellent (Both Unique taste, Dr. S. S. Adkar (Pan Herbs the drinks taste charming taste. Ltd. , Mumbai) excellent) 02/03/2001 P. L. NARAYANA, (Chief Tasted"Beer and Wish commercial M. U. Indo-Norwegian, Soft Drink". success. Programme) Tastes fabulous. -P. L. Narayana. 30/06/2001 Mr. RAVINDRA H. Excellent Taste It is really good CHAVAN, product which B-702, Shreenath I, Jaydeep meets regular beer Nagar, Bhandup (E), Mumbai product available in 400 042 market. Moreover, TEL-022-5919166 herbal beer is non- toxic. We wish this product is launched in market for betterment of regular beer consumers. 30/06/2001 KAMAL KUMAR, 0-34, Very good taste Needs to be made New Mahavir Nagar, Outer commercial grade Ring Road, New Delhi-18 Beer. 05/07/2001 Prof. V. K. DAMODRAN Tastes good Colour may be Energy Management made more Specialist, Trivandrum 695'inviting". If alcohol 037 percentage is brought to standard beer levels, could penetrate global market. Other positive advantages may be enlightened. 06/07/2001 Mr. MOTI RAM SHARMA, I am very happy to 66, Anand Puri, Wd, No. 13. drink it because this P. O. Krishna Nagar, Mathura is purely Indian - 281004. Phone (565) 461626 manufactured item and this is great achievement opputunity for Indian drinks. 06/07/2001 Shailaja Chanda, Secretary Excellent Very soothing and ISRM, Ministry of Health & tasty. I enjoyed it. Family Welfare 16/07/2001 R. L. KAUL, Sr. FAAO, A nice taste. NBRI However, the original colouri it is tasted better than yellowish one. 19/07/2001 R. P. SRIVASTAVA. (Sr. Pleasant Taste and SPO NBRI) the smell too. However, original coloured one beer has been found more tasty rather than the yellow colour. 13/09/2001 D. N. AGARWAL It goes smooth, never fell drunk. Colour and smell is good. 13/09/01 D. K. UPRETI, Scientist Smooth, excellent (NBRI) taste similar to the beers common in Germany. 12/07/2001 Dr. R. A. MASHELKAR, DG, I loved the taste of CSIR this original beer-I hope it becomes a world class product and brings name to NBRI/CSIR 28/07/2000 Hon. State Minister Shri. B. Tasty and I can Singh Rawat. (Ministry of realize it as Science and Technology, excellent drink. Govt. of India) Congratulations ! 30/07/2001 Professor B. R. Sant Wonderful taste. Consultant S&T, Nath Initially a bit Institute of Rural difficult to Development (NIRD-appreciate but after Hyderabad) 2-3 sips one starts liking. 31/07/2001 Dr. Sunita Kochhar Very good This beer is really very tasty. I could never drink the beer available in the market. But this beer is really excellent and refreshing. Congratulations to Dr. Shanta Mehrotra 31/07/2001 Dr. V. K. Kochhar Excellent Very refreshing and tasty. Once you have tasted it you want to have it again and again. Congratulations to Dr. Shanta Mehrotra. 1/08/2001 BHARAT PATAK, Shri Very good Keeping its qualities Purushotam Pandey in mind we can Deendayal Research Institute, think of a Chitrakoot commercial production for the product. 2/08/2001 Deepak Wahal Excellent Really an excellent and refreshing taste. I realised it after taking 2-3 sips. I would like to have it more and more. 02/02/2001 KAUSHAL KUMAR Very good It gives the real taste SRIVASTAV of Beer. 06/08/2001 RAJEEVKAPIL Good I wish the colour could be just like the conventional Beer. Otherwise, it is very good. 16/08/2001 ANIL KUMAR, COACDRI Excellent I relish the taste. 16/08/2001 ARUN WADH Excellent I like the taste. 16/08/2001 K. K. SINGH Excellent Taste. 20/08/2001 Dr. V. N. MISRA Excellent Taste 20/08/2001 Dr. G. N. QAZI Wonderful 20/08/2001 Dr. P. S. RAJUFANTASTIC 22/08/2001 Dr. USHA ZUTSHI Very wonderful. The flavor and taste is fantastic. It needs a little colour change to give a little kick enough when one tastes at it. 22/01/2001 Dr. S. K. Goyal, Chief Commissioner/Controller, Narcotics Factory Ghaziabad (U. P.) 23/02/2001 Dr. S. C. RAI, Hon. Mayor, I have tasted it. It is Lucknow very stimulating drink and is full of nutritional values. I congratulate the people who have introduced it. 23/08/2001 Dr. S. C. SHARMA, Emeritus I have the pleasure Scientist, NBRI (CSIR) to taste the herbal beer. The colour and taste are very pleasing. Heartiest congratulation to the Director and Team members. 31/08/2001 K. M. SAHNI, IAS-Director Invigorating and Thank you. Keep it General, Bureau of Indian Refreshing up. Standards, Manak Bhavan, New Delhi 31/08/2001 P. K. SARI (AR, Director Very Good Good Work; (Food & Agri. ) Bis, Manak Bhavan, New Delhi 31/08/2001 Dr. V. K. LAL, Dy. Director The product has Very good. (P'cognazy) CCRAS, very good taste Janalcpuri, New Delhi and may be a very useful herbal beer with medicinal properties 31/08/2001 SATISH CHANDER, ADG, Refreshing BIS, New Delhi 31/08/2001 Dr. S. K. SHARMA The taste is delicious, agreeable and good 03/01/2001 Dr. Nityanand, Dy. Director Delicious and (CDRI) refreshing 03/09/2001 Dr. J. K. Sharma, Director, Very pleasing & KFRI, Kerala refreshing drink almost similar to "Beer" ! Madhav Kashi, IDRC, Very refreshing An excellent Canada, 208 Dr. Bagh and promising example of substitute for beer innovation. P. MEHNDI NAND, Delicious and An innovation to be UNESCO, and Power Marg, refreshing popularized. New Delhi. D. R. BHATTARAI, HERBS Should be a mix Production and Processing drink. Co. Ltd. , Kathmandu, Nepal. Ms. SHRUTI SHARMA, FRI, Tastes like white This drink has a Dehradun wine. great potential within India itself and abroad if sold as Mango wine. P. EGNNATHAN, MSSRF, As beer It is taste & guddy Chennai & highly fermented better. S. P. SHARMA, Better than Beer Can get good KAMADHENU Foods, market in India. Saharanpur. VIRBALA SHAH, Quest Exhillarating Should make all Institute of Life Sciences, efforts to market it. Mulund (w), Mumbai 400 080 10/10/2001 Brig. P. P. SAINI AFMSD, A nourishing and Should be Lucknow-2 refreshing drink commercialized. KANWAR J. KAISER, It is refreshing and KAISER BASU process a change in our country for better, healthier Liquor. A. S. RATHORE, 24, Basant The taste of the Drink is having Vihar Colony, Lucknow drink is nice. good things for 226015 Good for health health. P. K. KHARE, DEVEN SRIVASTAVA, 601118, Om Nagar, Gurgaon. # 0124- 6660127 #9811148472 VIVEK SHARMA, Nice Good product Alimaman, Arjunganj, developed by NBRI Sultanpur, Rd. , Lucknow Dr. JANARDAN SINGH, Good and Unique Very refreshing and CIMAP, Lucknow invigorating drink. Ms. RIYA SINHA, NIF, Very tasty, as Can be developed as Ahmedabad good as been a marketeable product. Prof. PRAMOD TANDON, This healthy drink NEHU Shillong is so tasty. I wish it is promoted for commercial purposes soon. Dr. N. N. MEHROTRA, It has good taste National Institute of Rural similar to beer but Industrialization, less aerated. Very refreshing