Title:
FILLER FOR AFFINITY CHROMATOGRAPHY AND METHOD FOR ISOLATING IMMUNOGLOBULIN
Document Type and Number:
WIPO Patent Application WO/2011/118599
Kind Code:
A1
Abstract:
Provided are a filler for affinity chromatography which is highly tolerant to alkalis, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler wherein a protein represented by general formula (1) is immobilized on a support.
R-R2 (1)
In general formula (1), R represents an amino acid sequence consisting of 4 to 300 amino acid residues including a part consisting of 4 to 20 consecutive histidine residues; and R2 represents an amino acid sequence capable of binding to immunoglobulin, said amino acid sequence consisting of 50 to 500 amino acid residues including the Z domain of protein A, a fragment thereof or a mutant of the same, provided that R binds to the C- or N-terminus of R2.
More Like This:
Inventors:
TAMORI KOUJI (JP)
FUKUTA TETSUO (JP)
MIYAJI MASAAKI (JP)
WANG YONG (JP)
ABE TAKAYOSHI (JP)
OKANO YUSUKE (JP)
MOMIYAMA MASAKI (JP)
KAWAI TAKAHIRO (JP)
FUKUTA TETSUO (JP)
MIYAJI MASAAKI (JP)
WANG YONG (JP)
ABE TAKAYOSHI (JP)
OKANO YUSUKE (JP)
MOMIYAMA MASAKI (JP)
KAWAI TAKAHIRO (JP)
Application Number:
PCT/JP2011/056871
Publication Date:
September 29, 2011
Filing Date:
March 23, 2011
Export Citation:
Assignee:
JSR CORP (JP)
TAMORI KOUJI (JP)
FUKUTA TETSUO (JP)
MIYAJI MASAAKI (JP)
WANG YONG (JP)
ABE TAKAYOSHI (JP)
OKANO YUSUKE (JP)
MOMIYAMA MASAKI (JP)
KAWAI TAKAHIRO (JP)
TAMORI KOUJI (JP)
FUKUTA TETSUO (JP)
MIYAJI MASAAKI (JP)
WANG YONG (JP)
ABE TAKAYOSHI (JP)
OKANO YUSUKE (JP)
MOMIYAMA MASAKI (JP)
KAWAI TAKAHIRO (JP)
International Classes:
C07K17/08; C07K1/22; C07K19/00; C12N15/09; G01N30/88
Other References:
GRASLUND, SUSANNE ET AL.: "Single-vector three-frame expression systems for affinity- tagged proteins.", FEMS MICROBIOLOGY LETTERS, vol. 215, no. 1, 2002, pages 139 - 147, XP008090732
GRASLUND, SUSANE ET AL.: "A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products.", JOURNAL OF BIOTECHNOLOGY, vol. 99, no. 1, 2002, pages 41 - 50, XP002321144, DOI: doi:10.1016/S0168-1656(02)00158-X
MATEO, CESAR ET AL.: "One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 76, no. 3, 2001, pages 269 - 276
HARUYAMA TETSUYA ET AL.: "Protein layer coating method on metal surface by electrochemical process through genetical introduced tag.", BIOMATERIALS, vol. 26, no. 24, 2005, pages 4944 - 4947
See also references of EP 2574631A4
GRASLUND, SUSANE ET AL.: "A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products.", JOURNAL OF BIOTECHNOLOGY, vol. 99, no. 1, 2002, pages 41 - 50, XP002321144, DOI: doi:10.1016/S0168-1656(02)00158-X
MATEO, CESAR ET AL.: "One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 76, no. 3, 2001, pages 269 - 276
HARUYAMA TETSUYA ET AL.: "Protein layer coating method on metal surface by electrochemical process through genetical introduced tag.", BIOMATERIALS, vol. 26, no. 24, 2005, pages 4944 - 4947
See also references of EP 2574631A4
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
Patent business corporation Alga patent firm (JP)
Patent business corporation Alga patent firm (JP)
Download PDF:
Claims: