Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
FILTERS CONTAINING TERPENE-LOADED NANOFIBRES FOR ENHANCED BACTERICIDAL, FUNGICIDAL AND VIRUCIDAL ACTIVITY, PREPARATION METHODS AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/255885
Kind Code:
A1
Abstract:
Described herein are air/gas filter media containing terpene-loaded nanofibres for enhanced bactericidal, fungicidal and viricidal activity, preparation methods and applications thereof. More specifically, air/gas filter media for deactivating microorganisms and/or viruses. The air/gas filter media comprising one or more textile materials comprising one or more bactericidal and/or viricidal electrospun nanofibre layers. The pores within the nanofibre layers are dimensioned to prevent passage of airborne particles and upon contact with the nanofibre air filter, the bactericidal, fungicidal and/or viricidal actives deactivate the microorganisms and/or viruses within the airborne particles. Furthermore, the air filters have efficient breathability, washability performance and depending on the polymers used, may be environmentally friendly by being biodegradable and compostable.

Inventors:
BECKERMANN GARETH WESLEY (NZ)
HÖFLER GÜNTHER (NZ)
KARABULUT FABRICE NÖEL HAKAN (NZ)
Application Number:
PCT/NZ2022/050064
Publication Date:
December 08, 2022
Filing Date:
June 02, 2022
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
NANOLAYR LTD (NZ)
International Classes:
A41D13/11; B01D39/16; B32B5/26; B32B27/36; D01F1/10; D01F6/60; D01F6/62; D01F6/82; D01F8/12; D01F8/14
Domestic Patent References:
WO2021005258A22021-01-14
Foreign References:
CN108866820A2018-11-23
Other References:
PATIL, N.A. ET AL.: "Needleless electrospun phytochemicals encapsulated nanofibre based 3-ply biodegradable mask for combating COVID-19 pandemic", CHEMICAL ENGINEERING JOURNAL, vol. 416, 26 February 2021 (2021-02-26), pages 129152, XP086555291, DOI: https://doi.org/10.1016/j.cej 2021.129152
SOUZA MICHELLE A., OLIVEIRA JULIANO E., MEDEIROS ELITON S., GLENN GREGORY M., MATTOSO LUIZ H. C.: "Controlled Release of Linalool Using Nanofibrous Membranes of Poly(lactic acid) Obtained by Electrospinning and Solution Blow Spinning: A Comparative Study", JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, AMERICAN SCIENTIFIC PUBLISHERS, US, vol. 15, no. 8, 1 August 2015 (2015-08-01), US , pages 5628 - 5636, XP093012215, ISSN: 1533-4880, DOI: 10.1166/jnn.2015.9692
MELE ELISA: "Electrospinning of Essential Oils", POLYMERS, vol. 12, no. 4, 14 April 2020 (2020-04-14), XP093012219, DOI: 10.3390/polym12040908
GAO YUAN, BACH TRUONG YEN, ZHU YONGGANG, LOUIS KYRATZIS ILIAS: "Electrospun antibacterial nanofibers: Production, activity, and in vivo applications", JOURNAL OF APPLIED POLYMER SCIENCE, JOHN WILEY & SONS, INC., US, vol. 131, no. 18, 15 September 2014 (2014-09-15), US , pages n/a - n/a, XP055781907, ISSN: 0021-8995, DOI: 10.1002/app.40797
Attorney, Agent or Firm:
CREATEIP (NZ)
Download PDF:
Claims:
WHAT IS CLAIMED IS: 1. A filter media for deactivating microorganisms contained within an air or gas medium, comprising: one or more textile materials comprising one or more nanofibre layers containing functional actives, the functional actives having at least one of anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties, wherein the functional actives are bound to and/or contained within the nanofibres and wherein pores within the nanofibre layers are dimensioned to prevent passage of airborne particles, and upon contact with the nanofibre air/gas filter media, the functional actives deactivate the microorganisms within the air or gas medium. 2. The filter media as claimed in claim 1, wherein the pore size of the nanofibre material is between 50 – 800 nm in diameter. 3. A filter media for deactivating microorganisms within an air or gas medium, comprising: one or more textile materials comprising one or more nanofibre layers containing functional actives, wherein the functional actives are derived from terpenes, and have at least one of anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties, wherein the functional actives are configured to deactivate the microorganisms upon contact. 4. A filter media as claimed in claim 3, wherein the terpenes comprise terpenoids and isoterpenoids. 5. A filter media as claimed in claim 3 or claim 4, wherein the amount of terpenes contained within the nanofibre is in the range of 0.1 – 30 wt. %. 6. The filter media as claimed in any one of claims 3 to 5, wherein the terpenes are selected from any one of the following: manuka oil, tea tree oil, kanuka oil, eucalyptus, ginger, menthol, linalool, camphor, lemongrass, cinnamon, mint and ginko. 7. A compostable filter media configured to deactivate microorganisms within an air or gas medium, comprising: one or more textile nanofibre layers formed from-a bio-based material, wherein the at least one or more textile nanofibre layers comprise one or more functional active(s), the functional active(s) having at least one of anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties, wherein the functional active(s) are bound to and/or contained within the nanofibres and upon contact with the nanofibre layer, the functional active(s) are configured to deactivate the microorganisms upon contact, and wherein the filter media is configured to be substantially degradable under organic compostable conditions. 8. The filter media a claimed in claim 7, wherein the nanofibre layer comprises a polymer selected from any one of the following: poly lactic acid (PLA), polyhydroxyalkanote (PHA) and/or poly butylene adipate terephthalate (PBAT). 9. The filter media as claimed in any one of the preceding claims, wherein functional actives are incorporated into or bound to the nanofibres by surface coating methods or electrochemical spinning or electrospinning methods. 10. The filter media as claimed in any one of the preceding claims, wherein the filter media is utilised in air filtration systems, protective gear and/or face masks for protection against infectious pathogens, including bacteria, viruses, fungi and/or protozoa. 11. The filter media as claimed in any one of the preceding claims, wherein the nanofibres are electrostatically charged and attract, capture and retain small particles from air or gas by means of electrostatic attraction. 12. The filter media as claimed in any one of the preceding claims, wherein the nanofibres have a positive charge to attract negatively charged particles such as viruses, bacteria or other pollutants. 13. The filter media as claimed in claim 12, wherein the nanofibres have a static charge or voltage of between 1 to 15 kV. 14. The filter media as claimed in any one of the preceding claims, wherein a polymer of the nanofibres in the nanofibre layer is selected from any one of the following: EVOH (Ethylene vinyl alcohol), GPPS (General Purpose Polystyrene), PA11 (Polyamide 11), PA46 (Polyamide 46), PA6 (Polyamide 6), PA66 (Polyamide 66), PAI (Polyamide Imide), PAN (Polyacrylonitrile), PBI (Polybenzimidazole), PC (Polycarbonate), PCL (Polycaprolactone), PCU (Polycarbonate Urethane), PEI (Polyetherimide), PES (Polyether sulfone), PES (Polyethersulphone), PLA (Poly(lactic acid)), PLGA (Poly(lactic-co-glycolic) acid), PMMA (Poly(methyl methacrylate)), Polyamide XD10 (Xylylenesebacamide), PVB (Polyvinyl butyral), PVDF (Polyvinylidene fluoride), PVDF-HFP (Polyvinylidene Fluoride Copolymer), PVOH (Polyvinyl Alcohol), PVOH-AA – (Acetoacetyl Modified Polyvinyl Alcohol), PHA (polyhydroxyalkanoate), PBAT (poly butylene adipate terephthalate), PBS ( Polybutylene succinate), TPE (Thermoplastic Polyamide Elastomer), TPU (thermoplastic Polyurethane), and/or combinations thereof for effective absorption and carrying of functional active molecules.

15. The filter media as claimed in any one of the preceding claims, wherein polymer/s of the nanofibres in the nanofibre layer may contain soft polyether phase segments in between segments of a harder polymer phase, such that they are able to accommodate a broad range of volatile molecules acting as the functional actives and to facilitate the controlled release/diffusion of the functional actives into the environment. 16. The filter media as claimed in any one of claims 14 or 15, wherein the polymers utilise van der Waals forces and hydrogen bonding to contain the functional actives. 17. The filter media as claimed in any one of claims 14-16, wherein the polymers of the nanofibre are combined with additional processing aids to assist with the electrospinning process and to increase one or more of the functionality, electrostatic, electrical conductivity and hydrophobicity properties of the nanofibres. 18. The filter media as claimed in any one of the preceding claims, wherein the filter media comprises a multi-layer structure with the nanofibre layers situated between two or more layers of non-woven fabrics which are bound together utilising ultrasonic welding and/or thermal lamination processes. 19. The filter media as claimed in claim 18, wherein an activated carbon layer or blood barrier layer is added to the multi-layer filter media structure. 20. The filter media as claimed in any one of the preceding claims when dependent upon claim 1 or claim 7, wherein the functional actives used in the nanofibre layer are derived from terpenes, the terpenes being a class of natural unsaturated hydrocarbons that are produced predominately by plants and comprising of compounds with the formula (C5H8)n. 21. The filter media as claimed in claim 20, wherein the terpenes are selected from any one of the following: manuka oil, tea tree oil, kanuka oil, eucalyptus, ginger, menthol, linalool, camphor, lemongrass, cinnamon, mint and ginko. 22. The filter media as claimed in any one of claims 3 to 6 or claims 19 or 20, wherein the terpenes are utilised in combination with other functional actives and/or antimicrobial agents. 23. The filter media as claimed in claim 22, wherein other functional actives and antimicrobial agents comprise any of the following: cationic surfactants such as LAE (Lauroyl Arginate Ethyl), anionic surfactants such as SDS (Sodium Dodecyl Sulfate), quaternary ammonium compounds such as TBAC (Triethylbenzylammonium Chloride), anionic polyelectrolytes such as PSS (Poly[sodium 4- styrenesulfonate]), cationic polyelectrolytes such as PEI (Polyethylenimine), amine additives such as Benzalkonium Chloride, Tetrabutylammonium Bromide, Tetraethylammonium Bromide, N,N,N',N'',N''-Pentamethyldiethylenetriamine, Poly(dimethylsiloxane), Titanium(IV) oxide, Silver Acetate and Silver Nitrate, Nanosilver and/or Nanocopper. 24. The filter media as claimed in any one of the preceding claims, wherein the nanofibre layers contain between 1 to 30 wt.% of the functional actives. 25. The filter media as claimed in any one of the preceding claims, wherein the functional actives have bactericidal and viricidal properties to deactivate one or more of gram-positive bacteria, gram- negative bacteria, bacteriophage viruses, influenza viruses and coronaviruses. 26. The filter media as claimed in any one of the preceding claims, wherein the nanofibre layer is configured to provide for a controlled release and diffusion of the functional actives from the nanofibre layers, the controlled release and diffusion of the functional actives from the nanofibre layers provides a fragrance, aroma and/or a nasal decongestant. 27. The filter media as claimed in any one of the preceding claims, wherein the functional actives are retained in the nanofibre layer and maintain the at least one anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties after washing of the filter media. 28. The filter media as claimed in any one of the preceding claims, wherein the filter media exceeds ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % before and after at least 10x laundering cycles. 29. The filter media as claimed in any one of the preceding claims, wherein the filter media meets Level 1 airflow resistance requirements of ≤15 mm H2O before and after at least 10x laundering cycles. 30. The filter media as claimed in any one of the preceding claims, wherein the functional active has antibacterial functional properties and the filter media has an antibacterial activity reduction rate for Staphylococcus aureus and Klebsiella pneumoniae of greater than 99.5% after a contact time of 18 hours and at least 5x wash cycles. 31. The filter media as claimed in claim 24, wherein the antibacterial activity reduction rates of Staphylococcus aureus and Klebsiella pneumoniae after a contact time of 18 hours and at least 10x wash cycles are greater than 99%. 32. The filter media as claimed in any one of the preceding claims, wherein the nanofibre layer is formed using a PLA (Poly(lactic acid)) polymer and the functional active, the nanofibre layer is placed in- between two protective layers of spun-bonded PLA substrate. 33. The filter media as claimed in any one of the preceding claims, wherein the functional active comprises Manuka triketone. 34. The filter media as claimed in any one of the preceding claims, wherein the filter media has one or more of the following properties: (a) exceeds NIOSH - 42 CFR Part 84 N95 particle filtration efficiency requirements of ≥95%; (b) meets N95 inhalation and exhalation requirements of <314Pa and <245Pa, respectively; (c) exceeds ASTM F2100 Level 2 particle filtration efficiency requirements of ≥98% for 0.1 µm sized particles and shows >99.9% filtration efficiency for 0.3 µm sized particles; (d) exceeds ASTM F2100 Level 2 differential pressure requirements of <6.0 mm H2O/cm2; (e) exceeds ASTM F2100 Level 2 bacterial filtration efficiency requirements of >98%; (f) has a >99.9 viral filtration efficiency; and/or (g) exceeds ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % and meets Level 1 airflow resistance requirements of ≤15 mm H2O. 35. The filter media as claimed in any one of the preceding claims, wherein the nanofibre layer has antibacterial activity against a range of bacteria types, with reduction rates of at least 99.5% for Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae after a contact time of 18 hours. 36. The filter media as claimed in any one of the preceding claims, wherein the nanofiber layer has antiviral activity against a range of viruses, with reduction rates of at least 99.0% for Influenza H1N1, Human coronavirus 229E and Human Coronavirus SARS-CoV-2 (Delta Variant) after a contact time of 2 hours. 37. The filter media as claimed in any one of the preceding claims, wherein the nanofibre layer has antifungal activity and, wherein there is zero Aspergillus niger growth after a test period of 28 days. 38. The filter media as claimed in any one of the preceding claims wherein the filter media is at least 70% biodegradable by weight (wt.%) and/or at least 90% compostable by weight (wt.%). 39. The filter media as claimed in any one of the preceding claims wherein the filter media is configured to be substantially degradable under organic compostable conditions.

Description:
FILTERS CONTAINING TERPENE-LOADED NANOFIBRES FOR ENHANCED BACTERICIDAL, FUNGICIDAL AND VIRUCIDAL ACTIVITY, PREPARATION METHODS AND APPLICATIONS THEREOF TECHNICAL FIELD Described herein are filter media containing terpene-loaded nanofibres for enhanced bactericidal, fungicidal and viricidal activity, preparation methods and applications thereof. More specifically, air/gas filter media for deactivating microorganisms and/or viruses. The air/gas filter media comprising one or more textile materials comprising one or more bactericidal and/or viricidal electrospun nanofibre layers. The pores within the nanofibre layers are dimensioned to prevent passage of airborne particles and upon contact with the nanofibre filter, the bactericidal, fungicidal and/or viricidal functional actives deactivate the microorganisms and/or viruses within the airborne particles. Furthermore, the air /gas filters have efficient breathability, washability performance and depending on the polymers used, may be environmentally friendly by being biodegradable and compostable. BACKGROUND ART The air we breathe contains varying amounts of particles such as dust, dirt pollen, smog noxious fumes, bacteria and viruses. Airborne transmission of microbes and viruses is fast becoming one of the largest health threats of the industrial world. For example, health officials have advised that the coronavirus spreads through transmission by inhaling very fine respiratory droplets and aerosolised particles, as well as through contact with sprayed droplets or touching contaminated hands to one’s mouth, nose or eyes. In fact, the airborne coronavirus can be inhaled even when one is more than six feet away from an infected individual. Many persons, because of their jobs, lifestyle or location, must expose themselves to airborne particulate matter or fumes that they are allergic to, or which can be injurious to their health. The size of the particles is a main determinant of where in the respiratory tract the particle will come to rest when inhaled. Larger particles are generally filtered in the nose and throat via cilia and mucus, but particulate matter smaller than about 10 micrometres, referred to as PM10, can settle in the bronchi and lungs and cause health problems. The 10 micrometre size does not represent a strict boundary between respirable and non- respirable particles, but has been agreed upon for monitoring of airborne particulate matter by most regulatory agencies. Because of their small size, particles in the order of ~10 micrometres or less (PM10) can penetrate the deepest part of the lungs such as the bronchioles or alveoli. Similarly, so-called fine PM, particles smaller than 2.5 micrometres, PM2.5, tend to penetrate into the gas exchange regions of the lung (alveolus), and very small particles (< 100 nanometres) may pass through the lungs to affect other organs. Some persons are allergic to certain pollens or dust occurring naturally in the air and manifest this by an allergic reaction known as Hay Fever. Quite often, if uncontrolled, this allergic reaction progresses to a serious sinus condition or asthma. Also, the purity or quality of air may be affected by the presence of infectious airborne contaminates. Face masks are used to combat air pollution and contaminates that affect the quality of air. A conventional face mask is usually manufactured out of a piece of material that aims to filter out particulate material and seals or contacts against the face. One example is a mask that is manufactured out of cotton material, with the filter either being just a tiny square inside the cotton mask or the entire mask itself. Nevertheless, these types of mask are not particularly effective at filtering out particulate matter. Furthermore, these types of mask may only address air particulates and not bacteria or viruses. However, a number of anti-bacterial masks and the like have been developed. For example, patent CN 105455254B discloses nanofibre masks capable of effectively blocking . with nanometre sized antibacterial ingredients. The mask is comprised firstly of a nonwoven fabric, coarse filter layer, high efficiency filter made of nanofibres and secondly of a nonwoven fabric. The layers are bonded by ultrasonic welding methods. The antimicrobial coating is on the surface of the first nonwoven fabric. The antibacterial ingredients are selected from silver nanoparticles, a water-soluble chitosan powder or sodium alginate powder. The concentration of silver nanoparticles is 1-20%, the concentration of chitosan is 1-20% and the concentration of sodium alginate is 1-8%. All use water as solvent. The nanofibre layer is selected from polyacrylonitrile, polyurethane, polystyrene, nylon 6, nylon 66, or polyvinyl alcohol. Patent IN 202041024059A discloses a composition for preparing electrospun polymeric nanofibre mat for preparing face mask for antimicrobial use comprising polymeric resin material 5-90%; curcumin powder 0.5-40% and Nilavembu powder 0.05-25%, wherein the polymeric resin material is selected from poly (methyl methacrylate), poly (vinyl acetate) and/or combinations thereof. Patent IN 3254MUM2013 discloses a nanofibre based antimicrobial face mask for protection against viruses and a process of preparing the face mask thereof. In particular, a face mask for protection against viruses with claimed virus filtration efficiency of > 99%. The antimicrobial composite solution for electrospinning is prepared by adding a nanoparticle solution into a polymer electrospinning solution, wherein the resultant metal content is in the range 1 to 200 ppm. Combinations of one or more nonwoven and composite layers of one or more nanofibre layers on a melt blown substrate are stitched, such that the combination of layers provide for an antimicrobial face mask. The nanofibre layers are prepared from groups of materials selected from synthetic polymers such as Nylon 6, Nylon 66, Polyvinyl alcohol, polymethylmethacrylate, polyvinylidine fluoride, polyvinylidine chloride, cellulose ether, cellulose acetate, natural polymers such as chitosan, and derivatives of chitosan. The antimicrobial agents are selected from compounds or metals such as silver, zinc, copper or antibiotic compound, preferably at least 2 ppm silver content in the resultant fibre. The antimicrobial face mask is used for protection against flu viruses such as swine flu, avian flu and other microbes, where the pathogens are killed when they come in contact with the face mask’s surface during the process of filtration. US20180117370A1 discloses a mask having a built-in adsorptive membrane for adsorbing foreign substances contained in air, as well as containing a hanger band fixed to the mask body to be hung and fixed to the ear. The adsorptive membrane comprises of a support member wherein the absorptive member is made by accumulating ion exchange nanofibres for adsorbing ionic foreign substances. The foreign substances contained in the air may be one of pathogenic microorganisms, allergens, industrial dusts, fine dusts caused by yellow sand, viruses, and bacteria. Ion exchange nanofibres can be cation exchange nanofibres or anion exchange nanofibres. At least one of the support members and the first adsorptive member comprises stitched silver yarn. The ion exchange nanofibres are coated with oil. The second adsorptive member has a nanofibre web structure formed by electrospinning a spinning solution prepared by dissolving the silver nanomaterial in an organic solvent together with a fibre formability polymer material. Patent KR1020100003826A describes a dustproof mask for virus removal and facilitates respiration by low inspiratory resistance. The mask contains a nano-fibre web is comprised of base material and contains an antiviral agent of between 0.1 to 5.0 wt.%. The antiviral agent is selected from any one of hydroxy acid, X- type zeolite, pyrimidine basics, thiabendazole, metal, metal oxide, pyrroline acid salts, polyphosphate, silicate, aluminate, tungstenate, vanadate, molybdate, antimonite, and benzoate. Patent KR100536459B1 discloses a nanofibre web prepared by dissolving cellulose acetate and silver nitrate in a solvent and then electrospinning nanofibres for use in air purification and water treatment filters. The silver nitrate content in the cellulose acetate solution is between 0.001% and 10 wt.%. Patent CN107051232B discloses an air filtration membrane which has the function of killing bacteria and removing formaldehyde. The air filtration membrane is a three-layer nanofibre membrane composite structure, wherein the upper layer is an activated carbon nanofibre membrane, the middle layer is a pure TiO2 nanofibre membrane, and the lower layer is a nano-silver antibacterial fibre membrane. The activated carbon nanofibre membrane and nano-silver antibacterial fibre membrane both utilise polyvinylpyrrolidone as a polymer matrix. The diameter of activated carbon particles is 200 nm and these particles are dispersed in ethanol before adding to a blend of polyvinylpyrrolidone/water to make an electrospinning solution. The diameter of the nano-silver particles is 50 nm and these are dispersed in ethanol before adding to a blend of polyvinylpyrrolidone/water to make an electrospinning solution. It is claimed that the filter membrane has a filtration efficiency ≥ 99%, an air permeability ≥20 cm/s, and a filtration resistance <110 Pa. Patent CN108771976A discloses a method for preparing a fibre membrane for bacteria inhibition and filtration degradation based on electrostatic spinning. An electrospinning solution is made by mixing the following: 1 wt.% glucomannan solution, 1 wt.% citric acid solution, 10 wt.% polyvinyl alcohol solution and zinc oxide nanoparticles. Patent CN105688349A discloses an anti-virus mask comprising a spun-bonded fibre layer, a melt-blown fibre layer, a nanofibre layer and a second spun-bonded fibre layer, all of which are sequentially stacked. The nanofibre layer is prepared by dissolving the polymer in a polar aprotic solvent. The electrospinning solution comprises a conduction promoter selected from at least one of the following: nano-activated carbon particles, poly N-vinylpyrrolidone, polyethylene glycol, and polyvinyl alcohol. It further comprises antiviral and antibacterial agents having a mass fraction of 0.5% to 3%, selected from silver oxide, copper oxide, and zinc oxide, all with diameters from 100 nm to 150 nm. The outside layer of the face mask is made water resistant by means of a hydrophobic treatment. Patent CN111920120A discloses a nanofibre protective mask made by melt-spinning of hydrophobic materials under high voltages. Nano copper powders and/or nano silver powders are added to the liquid to be drawn. The nanofibre layer is laminated to the non-woven filter fabric material by means of hot pressing. Patent KR1020070097936A discloses an antibacterial filter media comprising nanofibre and nanosilver particles. The nanofibre material is made by an electrospinning method and the nanofibre is then coated with nanosilver particles using an electrospraying method. The nanosilver dispersed on the surface of the nanofibre material has a concentration ranging from 0.005-10% by weight of the nanofibre. Patent CN111450635A discloses a multilayer antibacterial nanofibre air-purification filter membrane wherein the first filter layer is coarse nanofibre, the second layer is fine nanofibre with an antibacterial layer in the middle. The claimed filter efficiency is up to 99.9%, and the killing rate of viruses and bacteria can reach 95% or above. The nanofibre material is specifically made from PVDF, PES, PSF, PAN, PVA, PI and/or combinations thereof. Nanofibres are produced by an electrospinning method. The antibacterial layer is a nanofibre film doped with silver-loaded graphene. Patent KR1020110046906A discloses a face mask comprising an inner layer, an outer layer, with a nanofibre layer in between. All the layers are consolidated by means of hot melt adhesive sealing. The nanofibres are made from PVDF or nylon. The antibacterial substances that are used are polyhexamethylene biguanide, chlorhexidine gluconate or polyhexamethylene biguanide. Patent CN102872653A discloses an antibacterial filtering material for facemask applications. It comprises an antibacterial agent, polymeric nanofibres and a supporting non-woven fabric. The structure has good breathability, and the filtration efficiency can be higher than 90% for 0.3 micrometre sized sodium chloride aerosol particles. The nanofibres can be made from the following: polyethersulfone, polyvinylidene fluoride, polyurethane, polyamide, polyacrylonitrile, polystyrene, polyvinyl alcohol, polylactic acid, polyethylene terephthalate, polyethylene or polypropylene. The following antibacterial agents can be used: silver, copper, zinc, silver oxide, copper oxide, cuprous oxide, zinc oxide, zirconium phosphate, nitric acid and combinations thereof. The polymer solutions containing antibacterial agent are spun onto the support nonwoven fabric by means of electrospinning. Patent CN104872865A discloses a biodegradable facemask made from nanofibres and two layers of a polypropylene non-woven fabric. The nanofibres are made from polybutylene succinate containing 0.2%- 0.6% nano silver using a bubble electrospinning method. Patent JP2018503755A discloses a protective facemask consisting of an ultrafine fibrous coating functionalised with a biocide for N95 level protection and bactericidal properties. The ultrafine fibrous coating consists of partially gelled submicron fibres woven with nanofibres. Partially gelled submicron fibres have a diameter of 100 to 1000 nm and nanofibres have a diameter of 10 to 99 nm. Partially gelled submicron fibres and nanofibres can be made from: cellulose acetate, polyamide 6, polystyrene, polyacrylonitrile, polyvinylpyrrolidone, polyvinyl alcohol, poly (lactic acid), poly (lactic acid-glycolic acid copolymer), polybutylene terephthalate, polyurethane, gelatine, chitosan or polyhydroxybutyrate- hydroxyvaleric acid copolymer. The biocidal agent comprises at least one of the following: silver, copper, copper oxide, titanium oxide, zinc oxide, iodine, triclosan and/or chlorhexidine. It is claimed that the protective mask shows a reduction in Gram-Positive bacteria, including Staphylococcus aureus, exceeding 99% within 5 minutes. The protective mask also shows a reduction in Gram-negative bacteria, including Pseudomonas aeruginosa, in an amount exceeding 99% within 5 minutes. Biocides are added into the polymer solution before electrospinning. Patent CN206714154U discloses a breathable mask capable of intercepting particulate matter. The non- woven fabrics are bonded by hot-pressing and the inner side of at least two non-woven fabrics is covered with a nanofibre layer. At least one nanofibre layer contains antibacterial particles. Antibacterial particles are selected from the following: nano silver, activated carbon or zeolite. The nanofibre layer is composed of any of the following polymers: polyurethane, polyvinyl alcohol, polyamide or polyvinylidene fluoride. Patent CN111235756A discloses a nanofibre mask comprising a non-woven fabric layer, a multi-layer drug- loaded composite-fibre membrane and another non-woven fabric. The multi-layer fibre membrane includes at least three layers: the inner and outer layers are made from melt-blown fibre or electrospun nanofibre, and the middle layer is a drug-loaded nano-microsphere fibre membrane. The polymer material is one or more of the following: polyethersulfone, polycaprolactone, polyvinylidene fluoride, polyamide, cellulose acetate, polyacrylonitrile, polylactic acid, polyurethane, polypropylene, polybutylene terephthalate, polyethylene, polyethylene terephthalate, polyvinylpyrrolidone, nylon 6, polyaniline, and polyvinyl alcohol. The polymer, drug-loaded nano-microspheres and a solvent are mixed before electrospinning. A needle-based electrospinning method is used. Patent CN103520999A discloses an antibacterial filter comprising a nonwoven fabric support layer, an antimicrobial fibre and microfibre blended layer, and a nanofibre filter layer. The antimicrobial fibre consists of 99.9-98 parts polymer and 0.1-2 parts antimicrobial agent. The antimicrobial agents are selected from the following: inorganic particles containing silver, copper or zinc ions or inorganic zeolite particles. The polymers used in making the antimicrobial fibre is selected from the following: polyacrylonitrile, polylactic acid, polyvinyl alcohol or polyvinylpyrrolidone. The antimicrobial agents are added into the polymer solution before electrospinning. The polymers for making the nanofiber filter layers are selected from the following: polyvinylpyrrolidone, polyvinyl acetate, polylactic acid, polymethyl methacrylate, polyacrylonitrile or nylon 6. Patent CN111593493A discloses a composite nanofibre membrane, which includes the following steps: preparing a graphene oxide dispersion; adding nano silica powder and polyacrylonitrile to the graphene oxide dispersion, and then adding metal ions. By adding metal ions, the composite nanofibre membrane exhibits antibacterial properties while filtering small aerosols. One or two of the following metal ions are used: Ag, Cu, Fe or W. The nanofibers are produced using needle-based electrospinning methods. From the above, it can be seen that very few nanofibre air/gas filters and facemasks have both bactericidal and viricidal functionalities. Therefore, they are not able to actively kill viruses and bacteria that are captured by the filter media. As a result of this, a used facemask or air filter may harbour or promote the proliferation of viruses and bacteria leading to secondary infections of users who handle or breathe through these contaminated items. Furthermore, if a bactericidal and/or viricidal additive is utilised, they are typically hazardous, toxic or harmful to human health and/or the environment (See for example, nano silver and nano copper additives, and chemical biocidal additives). Therefore, from the above it would be useful to have an air/gas filter media for enhanced bactericidal and viricidal activity or at least to provide the public with a useful choice. Further aspects and advantages of the air/gas filter media and its usage will become apparent from the ensuing description that is given by way of example only. SUMMARY Described herein are air/gas filter media containing terpene-loaded nanofibres for enhanced bactericidal, viricidal and antifungal activity, preparation methods and applications thereof. More specifically, air/gas filter media for deactivating microorganisms and/or viruses and/or fungi. The air/gas filter media comprising one or more textile materials comprising one or more bactericidal and/or viricidal electrospun nanofibre layers. The pores within the nanofibre layers are dimensioned to prevent passage of airborne particles and upon contact with the nanofibre air filter, the bactericidal and/or viricidal actives deactivate the microorganisms and/or viruses within the airborne particles. Furthermore, the air/gas filters have efficient breathability, washability performance and depending on the polymers used, may be environmentally friendly by being biodegradable and compostable. In a first aspect there is provided a filter media for deactivating microorganisms contained within an air or gas medium, comprising: one or more textile materials comprising one or more nanofibre layers containing functional actives, the functional actives having at least one of anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties, wherein the functional actives are bound to and/or contained within the nanofibres and wherein pores within the nanofibre layers are dimensioned to prevent passage of airborne particles, and upon contact with the nanofibre air/gas filter media, the functional actives deactivate the microorganisms within the air or gas medium. In a second aspect there is provided a method of manufacturing an air/gas filter media as substantially described above. In a third aspect there is provided a use of an air/gas filter media for capturing, retaining and deactivating microorganisms and/or viruses as substantially described above. In a fourth aspect there is provided a filter media for deactivating microorganisms within an air or gas medium, comprising: one or more textile materials comprising one or more nanofibre layers containing functional actives, wherein the functional actives are derived from terpenes, and have at least one of anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties, wherein the functional actives are configured to deactivate the microorganisms upon contact. In a fifth aspect there is provided a compostable filter media configured to deactivate microorganisms within an air or gas medium, comprising: one or more textile nanofibre layers formed from-a bio-based material, wherein at least one or more textile nanofibre layers comprise one or more functional active(s), the functional active(s) having at least one of anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties, wherein the functional active(s) are bound to and/or contained within the nanofibres and upon contact with the nanofibre layer, the functional active(s) are configured to deactivate the microorganisms upon contact, and wherein the filter media is configured to be substantially degradable under organic compostable conditions. A filter media, wherein the pore size of the nanofibre material is between 50 – 800 nm in diameter. A filter media, wherein the terpenes comprise terpenoids and isoterpenoids. A filter media, wherein the amount of terpenes contained within the nanofibre is in the range of 0.1 – 30 wt. %. A filter media, wherein the terpenes are selected from any one of the following: manuka oil, tea tree oil, kanuka oil, eucalyptus, ginger, menthol, linalool, camphor, lemongrass, cinnamon, mint and ginko. A filter media, wherein the nanofibre layer comprises a polymer selected from any one of the following: poly lactic acid (PLA), polyhydroxyalkanote (PHA) and/or poly butylene adipate terephthalate (PBAT). A filter media, wherein functional actives are incorporated into or bound to the nanofibres by surface coating methods or electrochemical spinning or electrospinning methods. A filter media, wherein the filter media is utilised in air filtration systems, protective gear and/or face masks for protection against infectious pathogens, including bacteria, viruses, fungi and/or protozoa. A filter media, wherein the nanofibres are electrostatically charged and attract, capture and retain small particles from air or gas by means of electrostatic attraction. A filter media, wherein the nanofibres have a positive charge to attract negatively charged particles such as viruses, bacteria or other pollutants. A filter media, wherein the nanofibres have a static charge or voltage of between 1 to 15 kV. A filter media, wherein a polymer of the nanofibres in the nanofibre layer is selected from any one of the following: EVOH (Ethylene vinyl alcohol), GPPS (General Purpose Polystyrene), PA11 (Polyamide 11), PA46 (Polyamide 46), PA6 (Polyamide 6), PA66 (Polyamide 66), PAI (Polyamide Imide), PAN (Polyacrylonitrile), PBI (Polybenzimidazole), PC (Polycarbonate), PCL (Polycaprolactone), PCU (Polycarbonate Urethane), PEI (Polyetherimide), PES (Polyether sulfone), PES (Polyethersulphone), PLA (Poly(lactic acid)), PLGA (Poly(lactic-co-glycolic) acid), PMMA (Poly(methyl methacrylate)), Polyamide XD10 (Xylylenesebacamide), PVB (Polyvinyl butyral), PVDF (Polyvinylidene fluoride), PVDF-HFP (Polyvinylidene Fluoride Copolymer), PVOH (Polyvinyl Alcohol), PVOH-AA – (Acetoacetyl Modified Polyvinyl Alcohol), PHA (polyhydroxyalkanoate), PBAT (poly butylene adipate terephthalate), PBS ( Polybutylene succinate), TPE (Thermoplastic Polyamide Elastomer), TPU (thermoplastic Polyurethane), and/or combinations thereof for effective absorption and carrying of functional active molecules. A filter media, wherein polymer/s of the nanofibres in the nanofibre layer may contain soft polyether phase segments in between segments of a harder polymer phase, such that they are able to accommodate a broad range of volatile molecules acting as the functional actives and to facilitate the controlled release/diffusion of the functional actives into the environment. A filter media, wherein the polymers utilise van der Waals forces and hydrogen bonding to contain the functional actives. A filter media, wherein the polymers of the nanofibre are combined with additional processing aids to assist with the electrospinning process and to increase one or more of the functionality, electrostatic, electrical conductivity and hydrophobicity properties of the nanofibres. A filter media, wherein the filter media comprises a multi-layer structure with the nanofibre layers situated between two or more layers of non-woven fabrics which are bound together utilising ultrasonic welding and/or thermal lamination processes. A filter media, wherein an activated carbon layer or blood barrier layer is added to the multi-layer filter media structure. A filter media, wherein the functional actives used in the nanofibre layer are derived from terpenes, the terpenes being a class of natural unsaturated hydrocarbons that are produced predominately by plants and comprising of compounds with the formula (C5H8)n. A filter media, wherein the terpenes are selected from any one of the following: manuka oil, tea tree oil, kanuka oil, eucalyptus, ginger, menthol, linalool, camphor, lemongrass, cinnamon, mint and ginko. A filter media, wherein the terpenes are utilised in combination with other functional actives and/or antimicrobial agents. A filter media, wherein other functional actives and antimicrobial agents comprise any of the following: cationic surfactants such as LAE (Lauroyl Arginate Ethyl), anionic surfactants such as SDS (Sodium Dodecyl Sulfate), quaternary ammonium compounds such as TBAC (Triethylbenzylammonium Chloride), anionic polyelectrolytes such as PSS (Poly[sodium 4-styrenesulfonate]), cationic polyelectrolytes such as PEI (Polyethylenimine), amine additives such as Benzalkonium Chloride, Tetrabutylammonium Bromide, Tetraethylammonium Bromide, N,N,N',N'',N''-Pentamethyldiethylenetriamine, Poly(dimethylsiloxane), Titanium(IV) oxide, Silver Acetate and Silver Nitrate, Nanosilver and/or Nanocopper. A filter media, wherein the nanofibre layers contain between 1 to 30 wt.% of the functional actives. A filter media, wherein the functional actives have bactericidal and viricidal properties to deactivate one or more of gram-positive bacteria, gram-negative bacteria, bacteriophage viruses, influenza viruses and coronaviruses. A filter media, wherein the nanofibre layer is configured to provide for a controlled release and diffusion of the functional actives from the nanofibre layers, the controlled release and diffusion of the functional actives from the nanofibre layers provides a fragrance, aroma and/or a nasal decongestant. A filter media, wherein the functional actives are retained in the nanofibre layer and maintain the at least one anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties after washing of the filter media. A filter media, wherein the filter media exceeds ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % before and after at least 10x laundering cycles. A filter media, wherein the filter media meets Level 1 airflow resistance requirements of ≤15 mm H2O before and after at least 10x laundering cycles. A filter media, wherein the functional active has antibacterial functional properties and the filter media has an antibacterial activity reduction rate for Staphylococcus aureus and Klebsiella pneumoniae of greater than 99.5% after a contact time of 18 hours and at least 5x wash cycles. A filter media, wherein the antibacterial activity reduction rates of Staphylococcus aureus and Klebsiella pneumoniae after a contact time of 18 hours and at least 10x wash cycles are greater than 99%. A filter media as claimed in any one of the preceding claims, wherein the nanofibre layer is formed using a PLA (Poly(lactic acid)) polymer and the functional active, the nanofibre layer is placed in-between two protective layers of spun-bonded PLA substrate. A filter media, wherein the functional active comprises Manuka triketone. A filter media, wherein the filter media has one or more of the following properties: (a) exceeds NIOSH - 42 CFR Part 84 N95 particle filtration efficiency requirements of ≥95%; (b) meets N95 inhalation and exhalation requirements of <314Pa and <245Pa, respectively; (c) exceeds ASTM F2100 Level 2 particle filtration efficiency requirements of ≥98% for 0.1 µm sized particles and shows >99.9% filtration efficiency for 0.3 µm sized particles; (d) exceeds ASTM F2100 Level 2 differential pressure requirements of <6.0 mm H2O/cm 2 ; (e) exceeds ASTM F2100 Level 2 bacterial filtration efficiency requirements of >98%; (f) has a >99.9 viral filtration efficiency; and/or (g) exceeds ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % and meets Level 1 airflow resistance requirements of ≤15 mm H2O. A filter media, wherein the nanofibre layer has antibacterial activity against a range of bacteria types, with reduction rates of at least 99.5% for Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae after a contact time of 18 hours. A filter media, wherein the nanofiber layer has antiviral activity against a range of viruses, with reduction rates of at least 99.0% for Influenza H1N1, Human coronavirus 229E and Human Coronavirus SARS-CoV-2 (Delta Variant) after a contact time of 2 hours. A filter media, wherein the nanofibre layer has antifungal activity and, wherein there is zero Aspergillus niger growth after a test period of 28 days. A filter media wherein the filter media is at least 70% biodegradable by weight (wt.%) and/or at least 90% compostable by weight (wt.%). A filter media wherein the filter media is configured to be substantially degradable under organic compostable conditions. Advantages of the above include an air/gas filter comprising nanofibre material that is dimensioned to prevent the passage of small airborne particles such as pollutants or infectious pathogens , including bacteria and viruses; the nanofibers are electrostatically charged to attract, capture and retain small particles from the air or gas; bactericidal and viricidal actives used in the nanofibres are taken from a large and diverse terpene class of naturally occurring non-toxic organic chemicals with additional functionality such as an aroma diffuser or nasal decongestant; the bactericidal and viricidal actives are bound to and/or contained within the nanofibres and thus results in a more durable, wash and wear resistant and longer lasting functional active i.e. the terpene actives cannot be washed out of the nanofibres and the nanofibres maintain their bactericidal functionality after washing; and a selection of polymers with soft and hard polyether phase segments between the layers that enable a wide range of functional actives for the controlled release/diffusion over a prolonged period of time. The filter media is extremely efficient with a lower pressure drop thus not affecting breathability when utilised as a face mask. The use of PLA material allows for biodegradability and compostability. BRIEF DESCRIPTION OF THE DRAWINGS Further aspects of the air/gas filter media, methods and uses thereof will become apparent from the following description that is given by way of example only and with reference to the accompanying figures in which: Figure 1A illustrates antiviral activity of XD10 nanofibre and PEBAX3 nanofibre film samples against Escherichia coli 25922. The bacteria recovered from each sample after overnight incubation is plotted for each sample. Each point represents an individual sample of 3 biological experiments. The horizontal bar is the median of samples, error bars represent the range; Figure 1B illustrates antimicrobial activity of nanofibre film samples against Escherichia coli 25922. The bacteria recovered from each sample after overnight incubation is plotted for each sample. Each point represents an individual sample of 3 biological experiments. The horizontal bar is the median of samples, error bars represent the range; Figure 2A illustrates antimicrobial activity of XD10 nanofibre and PEBAX3 nanofibre film samples against Staphylococcus aureus 6538. The bacteria recovered from each sample after overnight incubation is plotted for each sample. Each point represents an individual sample of 3 biological experiments. The horizontal bar is the median of samples, error bars represent the range; Figure 2B illustrates antimicrobial activity of nanofibre samples against Staphylococcus aureus 6538. The bacteria recovered from each sample after overnight incubation is plotted for each sample. Each point represents an individual sample of 3 biological experiments. The horizontal bar is the median of samples, error bars represent the range; and Figure 3 illustrates antimicrobial activity of nanofibre film samples against PhiX174. The virus recovered from each sample after overnight incubation is plotted for each sample. Each point represents an individual sample of 3 biological experiments. The horizontal bar is the median of samples, error bars represent the range; Figure 4A illustrates antimicrobial activity of washed nanofibre and film samples against Escherichia coli 25922. The bacteria recovered from each sample after overnight incubation is plotted for each sample. Each point represents an individual sample of 3 biological experiments. The horizontal bar is the median of samples, error bars represent the range; and Figure 4B illustrates antimicrobial activity of washed nanofibre and film samples against Staphylococcus aureus 6538. The bacteria recovered from each sample after overnight incubation is plotted for each sample. Each point represents an individual sample of 3 biological experiments. The horizontal bar is the median of samples, error bars represent the range. DETAILED DESCRIPTION As noted above, described herein are air/gas filter media containing terpene-loaded nanofibres for enhanced bactericidal, fungicidal and viricidal activity, preparation methods and applications thereof. More specifically, air/gas filter media for deactivating microorganisms and/or viruses and/or fungi. The air/gas filter media comprising one or more textile materials comprising one or more bactericidal and/or viricidal electrospun nanofibre layers. The pores within the nanofibre layers are dimensioned to prevent passage of airborne particles and upon contact with the nanofibre air filter, the bactericidal, fungicidal and/or viricidal actives deactivate the microorganisms and/or viruses within the airborne particles. Furthermore, the air/gas filters have efficient breathability and washability performance and depending on the polymers used, may be environmentally friendly by being biodegradable and compostable. The filter media may be used in a variety of applications such as face masks, respirator filters, cannister filters, HVAC filters, domestic, automotive, and industrial air/gas filters, garments, bedding, health sector personal protective equipment and curtains. This should not be seen as limiting for the type of applications that the filter media may be applied. For the purposes of this specification, the term ‘about’ or ‘approximately’ and grammatical variations thereof mean a quantity, level, degree, value, number, frequency, percentage, dimension, size, amount, weight or length that varies by as much as 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1% to a reference quantity, level, degree, value, number, frequency, percentage, dimension, size, amount, weight or length. The term ‘substantially’ or grammatical variations thereof refers to at least about 50%, for example 75%, 85%, 95% or 98%. The term 'comprise' and grammatical variations thereof shall have an inclusive meaning - i.e. that it will be taken to mean an inclusion of not only the listed components it directly references, but also other non- specified components or elements. The term ‘bio-based’ or grammatical variations thereof refers to a material, composition and/or substance that is derived, produced or synthesised in part or in whole from a renewable source such as a plant or animal source. For example, polylactic acid (PLA) which is produced by chemical and/or enzymatic conversion of corn starch is a bio-based polyester. Similarly, polyhydroxyalkanotes such as polyhydroxybutyric acid (PUB) are polyesters produced by bacterial or enzymatic conversion of carbohydrates such as sugar or glucose or canola oil. The term ‘compostable’ or grammatical variations thereof refers to a carbon based material that can be bacterially, enzymatically and/or hydrolytically degraded under conditions typically present in organic composting processes. Such conditions usually involve warm temperatures, a combination of aerobic and anaerobic bacterial activity and non-bacterial hydrolysis. Typically, a compostable material will be decomposed by bacterial and/or enzymatic action and/or by hydrolysis to a point where the compostable material is no longer recognizable or distinguishable from humus. However, the compostable material typically is not converted at this point to carbon dioxide and water plus inorganic residue. The term ‘biodegradable’ or grammatical variations thereof refers to a carbon based material that can be bacterially and/or enzymatically decomposed under conditions that are typically present in landfills. Such conditions usually include deep burial so that anaerobic bacterial and/or enzymatic activity typically will be the primary mode of decomposition. Such conditions typically do not include substantial non-bacterial hydrolysis, but hydrolysis may occur to some extent. Typically, the biodegradable material is converted primarily, substantially, essentially or completely to ultimate decomposition products such as carbon dioxide, water and inorganic residue. The bacterial and/or enzymatic decomposition will begin shortly after burial. Biodegradable polymers typically have hydrolysable linkages (e.g. ester, amide, ether bonds) along the polymer backbone that are susceptible to biodegradation. The more easily accessible these bonds are for microbial (bacteria, fungi, algae) attack, the faster the biodegradation. It should be appreciated that while all compostable material is biodegradable, not all biodegradable material is compostable. Although biodegradable materials return to nature and can disappear completely, they sometimes leave behind inorganic residue, on the other hand, compostable materials create humus as aforementioned that is full of nutrients and beneficial for plants. Compostable products are biodegradable, but with an added benefit. That is, when they break down, they release valuable nutrients into the soil, aiding the growth of trees and plants. In a first aspect there is provided an air/gas filter media for deactivating microorganisms contained within an air or gas medium, comprising: one or more textile materials comprising one or more nanofibre layers containing functional actives, the functional actives having at least one of anti-bacterial, anti-viral, anti-fungal, bactericidal, viricidal, and/or fungicidal functional properties, wherein the functional actives are bound to and/or contained within the nanofibres and wherein pores within the nanofibre layers are dimensioned to prevent passage of airborne particles, and upon contact with the nanofibre air/gas filter media, the functional actives deactivate the microorganisms within the air or gas medium. The functional actives may be incorporated into or bound to the nanofibres by surface coating methods or electrochemical spinning or electrospinning methods. Preferably, the functional actives may be incorporated into the nanofibers by needle-less electrospinning methods. In this way, this method allows for increased production capability. It has been found that functional actives or additives can sometimes block the needles in conventional electrospinning which can cause low production rates, inconsistency of nanofibre production and uneven deposition. An advantage of using needle-less electrospinning processes is that it overcomes these problems and allows for maximum production. For example, the production of functional face masks to fulfil the world’s current high demand for PPE. As above, the functional actives may have anti-bacterial, anti-fungal and/or anti-viral properties, or bactericidal, fungicidal and/or viricidal properties. In this way, the inherent antimicrobial and/or anti-viral properties of this layer can assist in real time deactivation of microorganisms enabling multiple use, longer effective life and the reduction of secondary infections. It is also envisaged that the functionality of the nanofibre material and interaction or loading with the functional active may correlate with the inhibition/killing rate to a specific virus or bacteria or fungi. The air/gas filter media may be applied to air filtration systems, protective gear and/or face masks for protection against infectious pathogens, including bacteria, viruses, fungi, protozoa and the like. This should not be seen as limiting for the type of applications that the air/gas filter media may be applied. For example, when used in a facemask, the nanofibre layer(s) may act as a barrier to the passage of microbes and both the user and bystander may be protected from microbes existing or entering the filter material. When used in an air filter, the nanofibre layer(s), may capture and prevent the spread of microbes through the filtered air. It is known that non-antimicrobial filters capture and harbour microbes and can contribute to their spread in the environment. It is therefore the object of the air filter media to actively kill the bacteria and viruses that they capture to prevent their repeated propagation and proliferation. The pore size of the nanofibre material may be between 50 – 800 nm in diameter. In this way, the nanofibre layers can be dimensioned to contain extremely small pores which may prevent the passage of small airborne particles such as pollutants or infectious pathogens, including bacteria, viruses, fungi and protozoa. The pressure drop/breathability, and to a lesser extent filtration efficiency, of a nanofibre filter media are dependent on the nanofibre diameters and pore sizes. These nanofibre diameters and pore sizes can be adjusted/controlled during the manufacturing process by manipulating one or more electrospinning parameters such as polymer type, polymer content in the electrospinning solution, spinning distance, voltage potential, additives added into the electrospinning solution and environmental conditions in the electrospinning chamber such as temperature and humidity. Furthermore, the nanofibres may be electrostatically charged and may attract, capture and retain small particles from air or gas by means of electrostatic attraction. In this way, the nanofibres may have a positive charge to attract negatively charged particles such as viruses, bacteria, pollutants, and the like. The nanofibres may have a static charge or voltage of between 1 to 15 kV. Conversely, the nanofibres may have a negative charge to attract positively charged particles. The nanofibres may have a static charge or voltage of between -1 to -15 kV. As aforementioned, the air/gas filter media material may contain a combined bactericidal and viricidal nanofibre layer. In this way, the bactericidal and viricidal actives may be bound to and/or contained within the nanofibres and hence are not present in the form of a surface coating that can be rubbed or washed off and may remain effective for several months. Alternately, the bactericidal and viricidal actives may be contained in a nanofibre surface coating. The filter media may exceed the ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % before as well as after 10x laundering cycles. In further embodiments, the filter media may also meet the Level 1 airflow resistance requirements of ≤15 mm H2O before and after 10x laundering cycles. The nanofiber component of the filter media has shown excellent antibacterial activity after 5x and 10x laundering cycles. After 5x washes, the reduction rates of Staphylococcus aureus and Klebsiella pneumoniae after a contact time of 18 hours were 99.996% and 99.834%, respectively. After 10x washes, the reduction rates of Staphylococcus aureus and Klebsiella pneumoniae after a contact time of 18 hours were 99.993% and 99.678%, respectively. The air/gas filter media may contain nanofibres manufactured from particular polymers that have been selected for their effectiveness at absorbing and carrying functional active molecules such as terpenes, wherein the terpenes may comprise terpenoids and isoterpenoids. In this way, the functional actives in combination with the polymers may enable the controlled release/diffusion of these actives over a prolonged period of time, resulting in a longer functional life and a wider application. The nanofibre polymer may selected from any one of the following, but should not be seen as limited to: EVOH (Ethylene vinyl alcohol), GPPS (General Purpose Polystyrene), PA11 (Polyamide 11), PA46 (Polyamide 46), PA6 (Polyamide 6), PA66 (Polyamide 66), PAI (Polyamide Imide), PAN (Polyacrylonitrile), PBI (Polybenzimidazole), PC (Polycarbonate), PCL (Polycaprolactone), PCU (Polycarbonate Urethane), PEI (Polyetherimide), PES (Polyether sulfone), PES (Polyethersulphone), PLA (Poly(lactic acid)), PLGA (Poly(lactic-co-glycolic) acid), PMMA (Poly(methyl methacrylate)), Polyamide XD10 (Xylylenesebacamide), PVB (Polyvinyl butyral), PVDF (Polyvinylidene fluoride), PVDF-HFP (Polyvinylidene Fluoride Copolymer), PVOH (Polyvinyl Alcohol), PVOH-AA – (Acetoacetyl Modified Polyvinyl Alcohol), PHA (polyhydroxyalkanoate), PBAT (poly butylene adipate terephthalate), PBS ( Polybutylene succinate), TPE (Thermoplastic Polyamide Elastomer), TPU (thermoplastic Polyurethane), and/or combinations thereof for effective absorption and carrying of functional active molecules. The air/gas filter media may contain biodegradable and/or compostable nanofibers made from biodegradable and/or compostable polymers used in conjunction with biodegradable and/or compostable backing/substrate materials. In embodiments where a filter media may comprise nanofibers manufactured from polylactic acid (PLA) with terpene additives and PLA substrate materials (including, but not limited to non-woven and woven cover layers and supporting layers), the resulting filter media may be biodegradable and/or compostable under the correct conditions. However, this should not be seen as limiting as conceivably other compostable polymers such as polyhydroxyalkanote (PHA) and/or poly butylene adipate terephthalate (PBAT) may be utilised. PLA consists of a repeating chain of lactic acid, which undergoes a two-step degradation process when placed under the environmental conditions typical of an industrial composter. Firstly, the moisture and heat in the compost pile attack the PLA chains and split them apart, creating smaller polymers, and finally, lactic acid. Microorganisms in compost and soil consume the smaller polymer fragments and lactic acid as nutrients. Since lactic acid is widely found in nature, a large number of organisms metabolize lactic acid. The end result of the process is carbon dioxide, water and also humus, a soil nutrient. PLA reacts with water and the rate of this chemical hydrolysis increases with temperature. This degradation process is temperature and humidity dependent. Unlike metal-ion releasing antimicrobial additives such as nano-silver and nano-copper which can persist in the environment for long periods of time, advantageously terpene additives such as Manuka triketone are naturally occurring and are naturally and fully biodegradable. Their antimicrobial properties do not persist in the environment after biodegradation. In this way, nanofibers containing terpene additives are thus able to fully biodegrade since the terpene additives contained within will themselves biodegrade and not inhibit the microorganisms in compost and soil that are responsible for breaking down the polymer. In preferred embodiments, the nanofibre polymer may be PLA. In this way, the air/gas filter may be biodegradable and compostable. As above, the filter may be constructed primarily, substantially or essentially of bio-based and/or biodegradable materials such that the filter may decompose at least 90 wt.% in a moderate amount of time in a landfill, in a burial site, in a compost site and/or in a garbage dump. Typically, the decomposition of the disposable filter may produce humus and residual material that is not recognisable as filter material. The humus and residual material may be of a chemical nature that may enable them to be assimilated by resident organisms present in soil, landfills, compost sites and dumps. Preferably, the decomposition may result primarily, substantially or essentially in further reduction of the filter materials into carbon dioxide, water and inorganic residue. As above, preferably the filter materials may be at least 70% biodegradable by weight (wt.%) and/or at least 90% compostable by weight (wt.%). Alternatively, as measured by volume percent (v/v%), preferably the filter materials may be at least 90% biodegradable and/or compostable by volume. The biodegradability and compostability may range substantially from 70, or 75, or 80, or 85, or 86, or 87, or 88, or 89, or 90, or 91, or 92, or 93, or 94, or 95, or greater (wt.%) and/or (v/v%). As aforementioned, the functional active molecules may be bound to and/or contained within the nanofibre polymers and are able to maintain their bactericidal and viricidal functionality after washing. These carrier polymers of the nanofibres may contain soft polyether phase segments in between segments of a harder phase, such that they are able to accommodate a broad range of volatile molecules and ensure their controlled release/diffusion into the environment. The carrier polymers may also utilise van der Waals forces and hydrogen bonding to contain the functional active molecules. In preferred embodiments, the nanofibre polymers may be combined with additional processing aids to assist with the electrospinning process and to increase the functionality, electrostatic, electrical conductivity and hydrophobicity properties of the nanofibres. The nanofibres may be situated between two or more layers of non-woven fabrics which may be bound together utilising ultrasonic welding and/or thermal lamination processes. The layers of non-woven fabrics may or may not have bactericidal and/or viricidal properties. An activated carbon layer or blood barrier layer may be optionally added to the multi-layer filter media structure. In preferred embodiments, the bactericidal and viricidal functional active molecules used in the nanofibre matrix may be derived from terpenes, a class of natural unsaturated hydrocarbons that are produced predominately by plants and comprising of compounds with the formula (C5H8)n. The inventors have found that products that use antimicrobial agents are either less effective (for example, the active coating wears off, or are potentially toxic). In the art, popular antimicrobial agents are metals and metal oxides containing silver or copper. However, the repeated use of these additives in medical and industrial applications can cause health and environment risks due to the leaching of the metal ions into water and the detachment of the nanoparticles into the environment. As above, terpenes, on the other hand, are naturally occurring and are non-toxic. The bactericidal and viricidal terpenes used in these air/gas filters and facemasks may be selected from, but not limited to any one of the following: manuka oil, tea tree oil, kanuka oil, eucalyptus, ginger, menthol, linalool, camphor, lemongrass, cinnamon, mint and ginko. The amount of terpenes may be in the range of 0.1 – 30 wt.% In one embodiment, a filter media comprising antimicrobial PLA nanofibers containing Manuka triketone placed in-between two protective layers of spun-bonded PLA substrate exhibits antibacterial and antiviral properties. The nanofiber component of the filter media may have antibacterial activity against a range of bacteria types, with reduction rates of 99.999% for Staphylococcus aureus, 99.957% for Escherichia coli and 99.82% Klebsiella pneumoniae after a contact time of 18 hours. The nanofiber component of the filter media may have antiviral activity against a range of viruses, with reduction rates of 99.0% for Influenza H1N1, 99.8% for Human coronavirus 229E and 99.7% for Human Coronavirus SARS-CoV-2 (Delta Variant) after a contact time of 2 hours. The terpene actives may be utilised in combination with other functional actives and/or antimicrobial agents. The other functional actives and antimicrobial agents may comprise, but should not be seen as limited to any of the following: cationic surfactants such as LAE (Lauroyl Arginate Ethyl), anionic surfactants such as SDS (Sodium Dodecyl Sulfate), quaternary ammonium compounds such as TBAC (Triethylbenzylammonium Chloride), anionic polyelectrolytes such as PSS (Poly[sodium 4- styrenesulfonate]), cationic polyelectrolytes such as PEI (Polyethylenimine), amine additives such as Benzalkonium Chloride, Tetrabutylammonium Bromide, Tetraethylammonium Bromide, N,N,N',N'',N''- Pentamethyldiethylenetriamine, Poly(dimethylsiloxane), Titanium(IV) oxide, Silver Acetate and Silver Nitrate, Nanosilver and/or Nanocopper. The nanofibres may contain functional actives between 1 to 30 wt.%. It has been found that this provides an effective amount of active to deactivate any pathogens with the required loading within the nanofibre material. In this way, the terpene actives have bactericidal and viricidal properties to deactivate gram- positive bacteria and gram-negative bacteria, along with bacteriophage viruses, influenza viruses and coronaviruses. The controlled release and diffusion of the terpene actives from the nanofibre filters may provide for a fragrance aroma diffuser. It is envisaged that the controlled release and diffusion of the terpene actives out of the nanofibre filters may result in a pleasant smell/fragrance/aroma for the user. When used in a face mask, the terpene containing nanofibre filter material may be able to mask the synthetic odour of the facemask fabrics, mask the smell of the wearer’s breath and also mask unpleasant odours in the surrounding air. When used in an air filter, the infused terpenes may be able to provide a pleasant fragrance to the clean, filtered air. Furthermore, the controlled release and diffusion of the terpene actives from the nanofibre filters may provide for a nasal decongestant. It is envisaged that when these terpenes are used in nanofibre filter materials, the release of these terpenes may have a decongestant effect. A wearer of a facemask containing such a material would thus have the nasal decongestant benefits offered by the controlled release of the terpenes and would be able to breathe easier. As is known the art, filtration efficiency and pressure drop (breathability) of a filter system such as a face mask is a compromise. However, the inventors have developed an efficient filter media with a high particle filtration efficiency and low pressure drop. The filter media may comprise antimicrobial PLA nanofibers containing a bactericidal, fungicidal and/or viricidal active such as Manuka triketone placed in-between two protective layers of spun-bonded PLA substrate and thus may meet international test standard requirements. The filter media may exceed NIOSH - 42 CFR Part 84 N95 particle filtration efficiency requirements of ≥95% and may meet the N95 inhalation and exhalation requirements of <314Pa and <245Pa, respectively. In one embodiment, the filter media may exceed ASTM F2100 Level 2 particle filtration efficiency requirements of ≥98% for 0.1 µm sized particles and may have >99.9% filtration efficiency for 0.3 µm sized particles. In one embodiment, the filter media may exceed the ASTM F2100 Level 2 differential pressure requirement of <6.0 mm H2O/cm 2 . In one embodiment, the filter media may exceed the ASTM F2100 Level 2 bacterial filtration efficiency requirement of >98%. As above, it has been found that filter media may show a >99.9 viral filtration efficiency. The filter media may also exceed the ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % and may meet the Level 1 airflow resistance requirements of ≤15 mm H2O. In a second aspect there is provided a method of manufacturing an air/gas filter media as substantially described above. In a third aspect there is provided a use of an air/gas filter media for capturing, retaining and deactivating microorganisms and/or viruses as substantially described above. The embodiments described above may also be said broadly to consist in the parts, elements and features referred to or indicated in the specification of the application, individually or collectively, and any or all combinations of any two or more said parts, elements or features. Further, where specific integers are mentioned herein which have known equivalents in the art to which the embodiments relate, such known equivalents are deemed to be incorporated herein as if individually set forth. WORKING EXAMPLES The above-described air/gas filter media, methods and uses are now described by reference to specific examples. EXAMPLE 1 Antimicrobial activity of nanofibers containing antimicrobial agents Materials and Methods The purpose of this investigation was to incorporate terpene antimicrobials into nanofibres, with the functional active ingredients being embedded in the nanofibres rather than being applied as a surface coating. The nanofibre materials would therefore have contact killing activity and exhibit low leaching of the actives. In this investigation, the electrospun nanofibre samples were tested for antimicrobial activity against model gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria and Bacteriophage virus PhiX174. The polymeric nanofibres were manufactured using electrospinning processes as would be known to those skilled in the art and need not be described in detail. The following materials (2cm x 2cm swatches of electrospun nanofibre on a backing substrate) were used in testing: • Polyethylene film control (not nanofibre); • Zoono TM control (sanitizer, not nanofibre); • Polyamide XD10 (XD10 NF No AM); • Polyamide XD10 with silver nanoparticles (XD10 NF Ag); • Polyamide XD10 with Manuka oil (XD10 NF MAN); • Polyamide XD10 with SDS (XD10 NF SDS, or XD10 (3) NF); • PEBAX (PEBAX3 NF Plain); • PEBAX with silver nanoparticles (PEBAX3 NF Ag); • PEBAX with Manuka oil (PEBAX3 NF MAN); • PEBAX with SDS (PEBAX3 NF with SDS, or PBX3 NF); • PEBAX/XD10 blend (PB3XD control); • PEBAX/XD10 blend with SDS (PB3XD NF); • PEBAX/XD10 blend with Manuka oil (PB3XD MAN 10); • PEBAX/XD10 blend with Tea Tree oil (PB3XD TT10); • PEBAX/XD10 blend with nano silver (PB3XD AG); • PEBAX/XD10 blend with nano copper (PB3XD CU); and • PVDF with Manuka oil (PVDF 4.9 MAN10) The sterile nanofibre samples were received and stored in screw cap plastic containers at room temperature. AATCC 100 is a commonly used US standard test method for antibacterial fabrics and has been used to validate the antimicrobial potency of fabrics for various uses, including wound dressings and surgical gowns. The test is relatively simple and can be adapted to test the antimicrobial activity against different bacterial and fungal species, as well as viruses. This method involves the inoculation of the material being tested, incubation and counting of the CFU’s (colony forming unit numbers of recovered bacteria/viruses). This test method is designed to quantitatively test the ability of fabrics and textiles to inhibit the growth of microorganisms or kill them, over a 24-hour period of contact, although the survival rates over different time periods with different re-inoculations can be done to simulate real-life conditions. The ATTCC 100 test protocol is described below: AATCC 100 Test Protocol: • Each sample was inoculated with 10 µl of Tryptic Soy Broth with 0.5% of Tween 20 containing 1 x 105 to 3 x 105 colony forming units (CFU) of either Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922 or Bacteriophage virus PhiX174. • The inoculated area was covered by a layer of ethanol-sterilised polyethylene film to keep the inoculated organism in contact with the treated area. • Ethanol sterilisation comprised sequential 1-minute immersions in 100%, 70% and 100% (by volume in water) ethanol followed by drying in a Class 2 Biosafety Cabinet. • Each sample was aseptically placed into a sterile petri-dish for incubation. • Negative and positive controls were set up in parallel. • An ethanol-sterilized piece of polyethylene was used as a negative control (no antimicrobial activity expected). • An ethanol-sterilized piece of polyethylene sprayed with Zoono TM and left to dry in a Class 2 Biosafety Cabinet was used as a positive control (antimicrobial activity expected). • Samples were incubated in a humid box at 37 °C for 24 hours. • After incubation samples were placed into sterile 50 ml tubes with 10 ml of Letheen broth. • Samples were vortexed and the wash volume was collected for each sample. • A dilution series was made with the wash in Letheen broth with aliquots plated to TSB agar (Difco) for enumeration of surviving bacteria after 24-48 hour incubation at 37°C. • The antimicrobial activity is presented as Log10 CFU recovered per sample; and is compared to the controls. • Each sample was tested in duplicate on three separate occasions, and all datapoints are plotted. • Some nanofibre samples were washed in water prior to testing to evaluate the wash resistance of the terpene loaded nanofibers. EXAMPLE 2 Results, Discussion and Conclusions With reference to Figures 1A, 1B, 2A and 2B, the bactericidal activity of nanofibre samples against Escherichia coli and Staphylococcus aureus can be shown. It can be seen from these Figures that the Polyamide XD10, PEBAX and PEBAX/XD10 based nanofibre samples containing antimicrobial agents SDS, Manuka oil, Tea Tree oil, nano-silver and nano-copper all demonstrated effective antimicrobial activity against Escherichia coli and Staphylococcus aureus, giving a total knockdown of >10 5 fold, and total eradication of the inoculum. It should be noted that the terpene additives (Manuka oil and Tea Tree oil) proved to be as good at eradicating bacteria as known antimicrobial agents such as nano-silver and nano-copper. The PVDF nanofibre samples, however, demonstrated poor activity, with variable activity against Escherichia coli and Staphylococcus aureus. Figure 3 shows the viricidal activity of nanofibre samples against Bacteriophage virus PhiX174. It can be seen that the PEBAX/XD10 and PVDF based nanofibre samples containing antimicrobial agents SDS, Manuka oil, Tea Tree oil, nano-silver and nano-copper all demonstrated effective antimicrobial activity against PhiX174, giving a total knockdown of >10 5 fold, and total eradication of the inoculum. Again, it should be noted that the terpene additives (Manuka oil and Tea Tree oil) proved to be as good at eradicating the bacteriophage virus as known antimicrobial agents such as nano-silver and nano- copper. Figures 4A and 4B show the bactericidal activity of washed nanofibre samples against Escherichia coli and Staphylococcus aureus. As can be seen from these Figures, the Polyamide XD10, PEBAX and PEBAX/XD10 based nanofibre samples containing SDS all demonstrated effective antimicrobial activity against Escherichia coli and Staphylococcus aureus despite being washed, giving a total knockdown of >10 5 fold, and total eradication of the inoculum. EXAMPLE 3 Filtration efficiency and breathability of a filter media containing antimicrobial PLA nanofibers with Manuka triketone The filtration efficiency and breathability (pressure drop) of a filter media comprising antimicrobial PLA nanofibers containing Manuka triketone placed in-between two protective layers of spun-bonded PLA substrate were evaluated in accordance with the following test methods: (a) NIOSH - 42 CFR Part 84, (b) ASTM F2100 and (c) ASTM F3502. (a) NIOSH - 42 CFR Part 84 - Respiratory Protective Devices Particle Filtration Efficiency and Airflow Resistance: This procedure was performed to evaluate the particle penetration and airflow resistance properties of filtration materials in accordance with NIOSH - 42 CFR Part 84 for Respiratory Protective Devices. A neutralized, poly-dispersed aerosol of sodium chloride (NaCl) was generated and passed through the test article. The performance of the test article was assessed by measuring the concentration of salt particles penetrating the test article compared to the challenge concentration entering the test article. The filtration performance and airflow resistance of each test article were calculated.

Note: Samples were not conditioned Results The filter media exceeded the NIOSH - 42 CFR Part 84 N95 particle filtration efficiency requirements of ≥95% as well as meeting the N95 inhalation and exhalation requirements of <314Pa and <245Pa, respectively. (b) ASTM F2100 - Standard Specification for Performance of Materials Used in Medical Face Masks Particle Filtration Efficiency: This procedure was performed to evaluate the non-viable particle filtration efficiency (PFE) of the test article. Monodispersed polystyrene latex spheres (PSL) were nebulized (atomized), dried, and passed through the test article. The particles that passed through the test article were enumerated using a laser particle counter. A one-minute count was performed, with the test article in the system. A one-minute control count was performed, without a test article in the system, before and after each test article. Control counts were performed to determine the average number of particles delivered to the test article. The filtration efficiency was calculated using the number of particles penetrating the test article compared to the average of the control values. During testing and controls, the airflow rate was maintained at 1 cubic foot per minute (CFM) ± 5%. The procedure employed the basic particle filtration method described in ASTM F2299, with some exceptions; notably the procedure incorporated a non-neutralized challenge. In real use, particles carry a charge, thus this challenge represents a more natural state. The non-neutralized aerosol is also specified in the FDA guidance document on surgical face masks. Results: Conclusions: The filter media exceeded the ASTM F2100 Level 2 particle filtration efficiency requirements of ≥98% for 0.1 µm sized particles and showed >99.9% filtration efficiency for 0.3 µm sized particles. Differential Pressure: The Delta P test is performed to determine the breathability of test articles by measuring the differential air pressure on either side of the test article using a manometer, at a constant flow rate. The Delta P test complies with EN 14683:2019+AC:2019. Results: Conclusions: The filter media exceeded the ASTM F2100 Level 2 differential pressure requirement of <6.0 mm H2O/cm 2 . Bacterial Filtration Efficiency (BFE): The BFE test is performed to determine the filtration efficiency of test articles by comparing the bacterial control counts upstream of the test article to the bacterial counts downstream. A suspension of Staphylococcus aureus was aerosolized using a nebulizer and delivered to the test article at a constant flow rate and fixed air pressure. The challenge delivery was maintained at 1.7 - 3.0 x 10 3 colony forming units (CFU) with a mean particle size (MPS) of 3.0 ± 0.3 μm. The aerosols were drawn through a six-stage, viable particle, Andersen sampler for collection. This test method complies with ASTM F2101-19 and EN 14683:2019+AC:2019. Results: Note: There were no detected colonies on any of the Andersen sampler plates for this test article Conclusions: The filter media exceeded the ASTM F2100 Level 2 bacterial filtration efficiency requirement of >98%. Viral Filtration Efficiency (VFE): The VFE test is performed to determine the filtration efficiency of test articles by comparing the viral control counts upstream of the test article to the counts downstream. A suspension of bacteriophage ΦX174 was aerosolized using a nebulizer and delivered to the test article at a constant flow rate and fixed air pressure. The challenge delivery was maintained at 1.1 - 3.3 x 103 plaque forming units (PFU) with a mean particle size (MPS) of 3.0 μm ± 0.3 μm. The aerosol droplets were drawn through a six-stage, viable particle, Andersen sampler for collection. The VFE test procedure was adapted from ASTM F2101.

Results: Conclusions: The filter media showed a >99.9 viral filtration efficiency. (c) ASTM F3502 - Standard Specification for Barrier Face Coverings Barrier Face Covering Evaluation: This procedure was performed to evaluate the particle penetration and airflow resistance properties of barrier face coverings as specified in ASTM F3502-21. This method is in compliance with 42 CFR Part 84 and is a modified version of the NIOSH method TEB-APR-STP-0059. A neutralized, poly-dispersed aerosol of sodium chloride (NaCl) was generated and passed through the test article. The performance of the test article was assessed by measuring the concentration of salt particles penetrating the test article compared to the challenge concentration entering the test article. The filtration performance of each test article was calculated. The airflow resistance was measured using the same method. Results: The final airflow resistance value for each test article was determined by subtracting out the background resistance from the system. Barrier Face Covering Minimum Performance Requirements per ASTM F3502: Conclusions: The filter media exceeded the ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % as well as meeting the Level 1 airflow resistance requirements of ≤15 mm H2O. EXAMPLE 4 Antimicrobial activity of PLA nanofibers containing Manuka triketone The antibacterial activity and antiviral activity of a filter media comprising antimicrobial PLA nanofibers containing Manuka triketone were evaluated using the following test methods: (a) ISO 20743:2013 (Determination of antibacterial activity of textile products); and (b) ISO 18184:2019 (Determination of antiviral activity of textile products). (a) ISO 20743:2013 (Antibacterial) Test Organism: Staphylococcus aureus (ATCC 6538P), and Klebsiella pneumoniae (AATCC 4352), Escherichia coli (ATCC 8739) Control Specimen: Control sample with a mass of 0.40 gm ± 0.05 gm Test Specimen: Treated sample with a mass of 0.40 gm ± 0.05 gm Sterilization: Autoclave at 121 °C and 103 kPa for 15 min. Test inoculum: 0.2 mL of test bacteria deposited onto the specimen Contact time: 18 hours Test Temperature: 37°C ± 1°C Antibacterial Activity (R) Calculation: A = (Ct – C0) - (Tt – C0) = (Ct – Tt) Where: A is the antibacterial activity value. C0 is the logarithm average of 3 bacterial colony forming unit (cfu) immediately after inoculation of the control specimen. Ct is the logarithm average of 3 bacterial colony forming unit (cfu) after specified contact time with the control specimen. Tt is the logarithm average of 3 bacterial colony forming unit (cfu) after specified contact time with the treated specimen.

Results: Conclusions: The nanofibre component of the filter media showed excellent antibacterial activity against a range of bacteria types, with reduction rates of 99.999% for Staphylococcus aureus, 99.957% for Escherichia coli and 99.82% Klebsiella pneumoniae after a contact time of 18 hours. (b) ISO 18184:2019 (Antiviral) Test Organism: Human coronavirus 229E (ATCC VR-740), Human Coronavirus SARS-CoV-2 (Delta Variant) and Influenza [H1N1] (ATCC VR-1469) Control Specimen: Control sample with a mass of 0.40 gm ± 0.05 gm Test Specimen: Treated sample with a mass of 0.40 gm ± 0.05 gm Sterilization: Autoclave at 121 °C and 103 kPa for 15 min. Test inoculum: 0.2 mL of test viral deposited onto the specimen Contact time: 2 hours Test Temperature: 37°C ± 1°C Antiviral Activity (R) Calculation: Mv = Log10 (Va) - Log10 (Vc) Where: Mv is the antiviral activity value Log10 (Va) is the logarithm average of 3 infectivity titre value immediately after inoculation of the control specimen Log10 (V b ) is the logarithm average of 3 infectivity titre value after specified contact time with the control specimen.

Results: Conclusions: The nanofibre component of the filter media showed excellent antiviral activity against a range of viruses, with reduction rates of 99.0% for Influenza H1N1, 99.8% for Human coronavirus 229E and 99.7% for Human Coronavirus SARS-CoV-2 (Delta Variant) after a contact time of 2 hours. EXAMPLE 5 Antifungal activity of PLA nanofibers containing Manuka triketone The antifungal/fungicidal activity of a filter media comprising antimicrobial PLA nanofibers containing Manuka triketone was evaluated using the following test method: ASTM G21 - Resistance of Synthetic Polymeric Materials to Fungi. (a) ASTM G21 - Resistance of Synthetic Polymeric Materials to Fungi Fungal resistance after 28 days: A control and test specimen of 2-inch X 2-inch size were cut for the testing. Fungal species was grown separately on Sabouraud dextrose agar for 7 - 14 days. The spore suspension of the fungi was prepared by pouring 10 mL of sterile DI water containing 0.5 mL of Tween 20 into the culture plate. The surface growth was gently scraped from the culture of test organism. The spore suspension was transferred into a centrifuge tube containing 25 mL of sterile DI water. The centrifuge tube was vortexed for one minute to break the spore clumps. The spore suspension was filtered to remove mycelial fragments. The spore suspension was washed three times in DI water by centrifugation and diluted to achieve a 1.0 x 10 6 spore/mL for each fungal species. Spore suspensions was then combined using equal volumes of resultant spore suspension. Both test sample and control were placed separately onto Sabouraud dextrose agar, and an even layer of spore suspension was sprayed onto each material sample. Plates were incubated at 29⁰C and examined weekly for 28 days. All tests were performed in triplicate. Results: ASTM G21: Aspergillus niger (ATCC 16888) Antifungal Rating: Conclusions: A filter media comprising a functional PLA nanofibre layer containing Manuka oil sandwiched in between two protective layers of a PLA spunbond nonwoven fabric was shown to be antifungal/fungicidal and showed no Aspergillus niger growth after a period of 28 days. EXAMPLE 6 Washability of filter media containing antimicrobial PLA nanofibers with Manuka triketone The effects of laundering on the filtration efficiency and antibacterial performance of a filter media comprising antimicrobial PLA nanofibers containing Manuka triketone placed in-between two protective layers of spun-bonded PLA substrate were evaluated using the following test methods: (a) ASTM F3502; and (2) ISO 20743. (a) ASTM F3502 - Standard Specification for Barrier Face Coverings Particle Filtration Efficiency and Airflow Resistance After 10x Laundering Cycles: This procedure was performed to evaluate the particle penetration and airflow resistance properties of barrier face coverings as specified in ASTM F3502-21. This method is in compliance with 42 CFR Part 84 and is a modified version of the NIOSH method TEB-APR-STP-0059. Face coverings for testing were either unwashed or laundered using the following washing procedure: Washing: 10x face coverings were placed into a mesh laundering bag and loaded into a Whirlpool front loading washer. Dunnage masks were added to the cycles as needed to simulate a full load. One Tide detergent pod was placed in the washer and the delicate wash cycle was selected and started. Drying: After each wash cycle was finished, the face coverings were set out to air-dry until completely dry. Visual Inspection: After each wash cycle and air-drying steps were completed, the face coverings were visually inspected to identify any physical damage and all observations were recorded. Samples were then tested. A neutralized, poly-dispersed aerosol of sodium chloride (NaCl) was generated and passed through each test article. The performance of the test article was assessed by measuring the concentration of salt particles penetrating the test article compared to the challenge concentration entering the test article. The filtration performance of each test article was calculated. The airflow resistance was measured using the same method. Results: Before Washing: After 10x Laundering Cycles: The final airflow resistance value for each test article was determined by subtracting out the background resistance from the system. Barrier Face Covering Minimum Performance Requirements per ASTM F3502: Conclusions: The filter media exceeded the ASTM F3502 Level 2 particle filtration efficiency requirements of ≥50 % before as well as after 10x laundering cycles. In addition to this, the filter media also met the Level 1 airflow resistance requirements of ≤15 mm H2O before and after 10x laundering cycles. (b) ISO 20743 - Determination of antibacterial activity of textile products The following procedure was performed to investigate the effects of 5x laundering cycles and 10x laundering cycles on the antibacterial functionality of the active nanofibre layer in the filter media. Laundering was performed in accordance with ISO 6330:2013 to the following test parameters: 3G: 30°C, gentle setting (wool silk synthetics). Test Organisms: Staphylococcus aureus (ATCC 6538P) and Klebsiella pneumoniae (AATCC 4352). Control Specimen: Control sample with a mass of 0.40 gm ± 0.05 gm Test Specimen: Treated sample with a mass of 0.40 gm ± 0.05 gm Sterilization: Autoclave at 121 °C and 103 kPa for 15 min. Test inoculum: 0.2 mL of test bacteria deposited onto the specimen Contact time: 18 hours Test Temperature: 37°C ± 1°C Antibacterial Activity (R) Calculation: A = (Ct – C0) - (Tt – C0) = (Ct – Tt) Where: A is the antibacterial activity value. C0 is the logarithm average of 3 bacterial colony forming unit (cfu) immediately after inoculation of the control specimen. Ct is the logarithm average of 3 bacterial colony forming unit (cfu) after specified contact time with the control specimen. Tt is the logarithm average of 3 bacterial colony forming unit (cfu) after specified contact time with the treated specimen. Results: Conclusions: The nanofibre component of the filter media showed excellent antibacterial activity after 5x and 10x laundering cycles. After 5x washes, the reduction rates of Staphylococcus aureus and Klebsiella pneumoniae after a contact time of 18 hours were 99.996% and 99.834%, respectively. After 10x washes, the reduction rates of Staphylococcus aureus and Klebsiella pneumoniae after a contact time of 18 hours were 99.993% and 99.678%, respectively. Aspects of the present invention have been described by way of example only and it should be appreciated that modifications and additions may be made thereto without departing from the scope of the description and claims herein.