FKBP TYPE PPIASE, EXPRESSION VECTOR, TRANSFORMANT, FUSED PROTEIN, METHOD OF PRODUCING FUSED PROTEIN, METHOD OF PRODUCING TARGET PROTEIN, METHOD OF PURIFYING KFBP TYPE PPIASE AND METHOD OF PURIFYING FUSED PROTEIN

Title:

Document Type and Number:

WIPO Patent Application WO/2005/063964

Kind Code:

A1

Abstract:

It is intended to produce a target protein at a high efficiency with the use of the action of FKBP type PPIase which shows a sufficient activity in a region from low temperature to ordinary temperature in usual biochemical environment. Owing to the action of FKBP type PPIase originating in a non-thermophilic archaebacterium belonging to methane-producing bacteria, a target protein is obtained as a normal protein. By a fused protein composed of FKBP type PPIase with the target protein or the coexpression of the FKBP type PPIase with the target protein, the target protein can be properly folded. By using a vector which can express the fused protein, the target protein can be produced at a high efficiency.

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Inventors:

IDENO AKIRA (JP)
NISHIGUCHI NAOKI (JP)
FURUTANI MASAHIRO (JP)

Application Number:

PCT/JP2004/019306

Publication Date:

July 14, 2005

Filing Date:

December 24, 2004

Export Citation:

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Assignee:

SEKISUI CHEMICAL CO LTD (JP)
IDENO AKIRA (JP)
NISHIGUCHI NAOKI (JP)
FURUTANI MASAHIRO (JP)

International Classes:

C07K16/40; C07K19/00; C12N1/21; C12N9/90; C12N15/61; (IPC1-7): C12N1/21; C07K16/40; C07K19/00; C12N9/90; C12N15/61

Foreign References:

JP2001066309A	2001-03-16
JP2002262883A	2002-09-17

Other References:

DATABASE GENBANK [online] 7 February 2003 (2003-02-07), DEPPENMEIER U, ET AL: "Methanosarcina mazei strain Goel, section 45 of 379 of the complete genome", XP002992035, Database accession no. (AE013263)
DATABASE GENBANK [online] 3 April 2002 (2002-04-03), GALAGAN J.E. ET AL: "Methanosarcina acetivorans str.C2A, section 326 of 534 of the complete genome", XP002992033, Database accession no. (AE010981)

Attorney, Agent or Firm:

Fujita, Takashi (Higashitenma 2-chome, Kita-k, Osaka-shi Osaka 44, JP)

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