Title:
FLAVIN-BINDING GLUCOSE DEHYDROGENASE VARIANT
Document Type and Number:
WIPO Patent Application WO/2017/195765
Kind Code:
A1
Abstract:
The present invention relates to FAD-GDH which comprises an amino-acid sequence represented by SEQ ID NO: 1 or 3, an amino-acid sequence which is at least 70% identical to the amino-acid sequence represented by SEQ ID NO: 1 or 3, or an amino-acid sequence having the deletion, substitution, or addition of one to several amino acids in such amino-acid sequence (the amino-acid sequence represented by SEQ ID NO: 1, or the amino-acid sequence which is at least 70% identical to the amino-acid sequence represented by SEQ ID NO: 1). The present invention is characterized by having an amino-acid substitution at positions corresponding to the following amino-acids: amino acid at position 175 in the amino-acid sequence depicted in SEQ ID NO: 1; amino acid at position 214 in the amino acid sequence depicted in SEQ ID NO: 1; amino acid at position 192 in the amino-acid sequence depicted in SEQ ID NO: 1; amino acid at position 212 in the amino-acid sequence depicted in SEQ ID NO: 1; amino acid at position 218 in the amino-acid sequence depicted in SEQ ID NO: 1; and/or amino acid at position 226 in the amino-acid sequence depicted in SEQ ID NO: 1. The present invention is also characterized in that the amino-acid sequence has improved thermal stability compared to that prior to the substitution. However, the present invention excludes FAD-GDH comprising an amino-acid sequence represented by SEQ ID NO: 28, 30 or 32.
Inventors:
MASAKARI YOUSUKE (JP)
HARA CHIAKI (JP)
HARA CHIAKI (JP)
Application Number:
PCT/JP2017/017494
Publication Date:
November 16, 2017
Filing Date:
May 09, 2017
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N15/09; C12M1/40; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32
Domestic Patent References:
| WO2013118799A1 | 2013-08-15 | |||
| WO2013118798A1 | 2013-08-15 | |||
| WO2013065623A1 | 2013-05-10 | |||
| WO2015099112A1 | 2015-07-02 | |||
| WO2012169512A1 | 2012-12-13 | |||
| WO2013065770A1 | 2013-05-10 | |||
| WO2015129475A1 | 2015-09-03 |
Foreign References:
| JP2015084676A | 2015-05-07 | |||
| JP2017000137A | 2017-01-05 |
Other References:
See also references of EP 3456823A4
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES et al. (JP)
Download PDF:
Previous Patent: METHOD AND DEVICE FOR TREATING WASTEWATER CONTAINING ORGANIC MATTER
Next Patent: CHARGED PARTICLE BEAM IRRADIATION DEVICE
Next Patent: CHARGED PARTICLE BEAM IRRADIATION DEVICE