Title:
FLAVIN ENZYME HAVING FLAVONOL 8-HYDROXYLASE ACTIVITY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/023583
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a novel flavonol 8-hydroxylase. The present invention relates to a flavin enzyme protein that has flavonol 8-hyroxylase activity, and a polynucleotide etc. that codes the flavin enzyme protein. The present invention provides: a polynucleotide that includes, for example, the base sequence represented by SEQ ID NO: 1 or 3; a polynucleotide that codes a protein comprising an amino acid sequence represented by SEQ ID No. 2; an expression vector and a transformant that include the polynucleotide; a plant screening method that causes yellow flowers to bloom by using the polynucleotide; a plant producing method that causes yellow flowers to bloom by introducing the polynucleotide into host cells; and a method for producing a flavin enzyme protein that has flavonol 8-hydroxylase activity, said method using the transformant.
Inventors:
SUZUKI HIDEYUKI (JP)
Application Number:
PCT/JP2011/068683
Publication Date:
February 23, 2012
Filing Date:
August 18, 2011
Export Citation:
Assignee:
SUNTORY HOLDINGS LTD (JP)
SUZUKI HIDEYUKI (JP)
SUZUKI HIDEYUKI (JP)
International Classes:
C12N15/09; A01H5/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12P17/06; C12Q1/68
Domestic Patent References:
| WO2006126294A1 | 2006-11-30 | |||
| WO2000044907A1 | 2000-08-03 | |||
| WO2005017147A1 | 2005-02-24 | |||
| WO1993001290A1 | 1993-01-21 | |||
| WO2008156211A1 | 2008-12-24 |
Foreign References:
| JP2010183875A | 2010-08-19 | |||
| JP2005287403A | 2005-10-20 | |||
| US6045996A | 2000-04-04 | |||
| US5858659A | 1999-01-12 |
Other References:
DATABASE GENBANK [online] 12 February 2009 (2009-02-12), "Monoxygenase, putative [Ricinus communis]", XP003031804, accession no. NCBI Database accession no. EEF29933
DATABASE GENBANK [online] 18 March 2004 (2004-03-18), "BP039673 Lotus corniculatus var. japonicus flower bud Lotus japonicus cDNA clone MFB078b04_f 3-, mRNA sequence", XP003031805, accession no. NCBI Database accession no. BP039673
HARBOME J.B.: "Comparative Biochemistry of the Flavonoids", 1967, ACADEMIC PRESS
KUNNUMAKKARA A.B. ET AL., BLOOD, vol. 109, 2007, pages 5112 - 5121
ANZELLOTTI D; IBRAHIM R.K., BMC PLANT BIOL, vol. 4, 2004, pages 20
HALBWIRTH H. ET AL., PLANT SCI., vol. 167, 2004, pages 129 - 135
LATUNDE-DATA A.O. ET AL., J. BIO. CHEM., vol. 276, 2001, pages 1688 - 1695
HALBWIRTH H.; STICH K., PHYTOCHEMISTRY, vol. 67, 2006, pages 1080 - 1087
SAMBROOK; RUSSELL: "Molecular Cloning; A Laboratory Manual", vol. 3, 2001, COLD SPRING HARBOR, LABORATORY PRESS
AUSUBEL: "Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
PROC. NAIL ACAD. SCI. USA, vol. 90, 1993, pages 5873
ALTSCHUL S. F. ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403
SAMBROOK; RUSSELL: "Molecular Cloning: A Laboratory Manual", vol. 3, 2001, COLD SPRING HARBOR LABORATORY PRESS
NUC. ACIDS. RES., vol. 10, 1982, pages 6487
PROC. NATL. ACAD. SCI. USA, vol. 79, 1982, pages 6409
GENE, vol. 34, 1985, pages 315
NUC. ACIDS. RES., vol. 13, 1985, pages 4431
PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 488
HALBWIRTH H; STICH K, PHOTOCHEMISTRY, vol. 67, 2006, pages 1080 - 1087
ROSSMANN FOLD; RAO S; ROSSMANN M, J MOL BIOL, vol. 76, no. 2, 1973, pages 241 - 56
MARIN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 337
JUNJI INOKOSHI ET AL., BIOCHEMISTRY, vol. 64, 1992, pages 660
HUSSAIN ET AL., GENE, vol. 101, 1991, pages 149
MACKENZIE, D. A. ET AL., APPL. ENVIRON. MICROBIOL., vol. 66, 2000, pages 4655 - 4661
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
J. BACTERIOLOGY, vol. 153, 1983, pages 163
"Methods in yeast genetics", 2000, A COLD SPRING HARBOR LABORATORY
"Methods in Yeast Genetics", A LABORATORY MANUAL (COLD SPRING HARBOR LABORATORY PRESS
HIROBUMI UCHIMIYA: "Manuals for Plant Gene Manipulation", 1990, KODANSHA SCIENTIFIC CO., LTD., pages: 27 - 31
NAGEL ET AL., MICROBIOL. LETT., vol. 67, 1990, pages 325
GELVIN, S.B. ET AL.: "Plant Molecular Biology Manual", ACADEMIC PRESS PUBLISHERS
"Shokubutsu Saibo Kogaku Nyumon", GAKKAI SHUPPAN CENTER, article "Seibutsu Kagaku Jikkenho", pages: 41
SAMBROOK; FRITSCH; MANIATIS: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SIEBEN V.J. ET AL., IET NANOBIOTECHNOLOGY, vol. 1, no. 3, June 2007 (2007-06-01), pages 27 - 35
LU N. ET AL., J. AGRIC. FOOD CHEM., vol. 56, no. 23, 2008, pages 11526 - 11532
ASAMIZU, PMB, vol. 54, 2004, pages 405 - 414
HALBWIRTH; STICH, PHYTOCHEMISTRY, vol. 67, 2006, pages 1080 - 1087
RAO S.; ROSSMANN M., J. MOL. BIOL., vol. 76, no. 2, 1973, pages 241 - 56
SATO S. ET AL., DNA RESEARCH, 2008, pages 1 - 13
TANAKA Y; FUKUCHI-MIZUTANI M; MASON JG: "Rose Encyclopedia", 2003, ELSEVIER, article "Cloned genes", pages: 341 - 347
See also references of EP 2615169A4
DATABASE GENBANK [online] 18 March 2004 (2004-03-18), "BP039673 Lotus corniculatus var. japonicus flower bud Lotus japonicus cDNA clone MFB078b04_f 3-, mRNA sequence", XP003031805, accession no. NCBI Database accession no. BP039673
HARBOME J.B.: "Comparative Biochemistry of the Flavonoids", 1967, ACADEMIC PRESS
KUNNUMAKKARA A.B. ET AL., BLOOD, vol. 109, 2007, pages 5112 - 5121
ANZELLOTTI D; IBRAHIM R.K., BMC PLANT BIOL, vol. 4, 2004, pages 20
HALBWIRTH H. ET AL., PLANT SCI., vol. 167, 2004, pages 129 - 135
LATUNDE-DATA A.O. ET AL., J. BIO. CHEM., vol. 276, 2001, pages 1688 - 1695
HALBWIRTH H.; STICH K., PHYTOCHEMISTRY, vol. 67, 2006, pages 1080 - 1087
SAMBROOK; RUSSELL: "Molecular Cloning; A Laboratory Manual", vol. 3, 2001, COLD SPRING HARBOR, LABORATORY PRESS
AUSUBEL: "Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
PROC. NAIL ACAD. SCI. USA, vol. 90, 1993, pages 5873
ALTSCHUL S. F. ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403
SAMBROOK; RUSSELL: "Molecular Cloning: A Laboratory Manual", vol. 3, 2001, COLD SPRING HARBOR LABORATORY PRESS
NUC. ACIDS. RES., vol. 10, 1982, pages 6487
PROC. NATL. ACAD. SCI. USA, vol. 79, 1982, pages 6409
GENE, vol. 34, 1985, pages 315
NUC. ACIDS. RES., vol. 13, 1985, pages 4431
PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 488
HALBWIRTH H; STICH K, PHOTOCHEMISTRY, vol. 67, 2006, pages 1080 - 1087
ROSSMANN FOLD; RAO S; ROSSMANN M, J MOL BIOL, vol. 76, no. 2, 1973, pages 241 - 56
MARIN ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 337
JUNJI INOKOSHI ET AL., BIOCHEMISTRY, vol. 64, 1992, pages 660
HUSSAIN ET AL., GENE, vol. 101, 1991, pages 149
MACKENZIE, D. A. ET AL., APPL. ENVIRON. MICROBIOL., vol. 66, 2000, pages 4655 - 4661
PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929
J. BACTERIOLOGY, vol. 153, 1983, pages 163
"Methods in yeast genetics", 2000, A COLD SPRING HARBOR LABORATORY
"Methods in Yeast Genetics", A LABORATORY MANUAL (COLD SPRING HARBOR LABORATORY PRESS
HIROBUMI UCHIMIYA: "Manuals for Plant Gene Manipulation", 1990, KODANSHA SCIENTIFIC CO., LTD., pages: 27 - 31
NAGEL ET AL., MICROBIOL. LETT., vol. 67, 1990, pages 325
GELVIN, S.B. ET AL.: "Plant Molecular Biology Manual", ACADEMIC PRESS PUBLISHERS
"Shokubutsu Saibo Kogaku Nyumon", GAKKAI SHUPPAN CENTER, article "Seibutsu Kagaku Jikkenho", pages: 41
SAMBROOK; FRITSCH; MANIATIS: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
SIEBEN V.J. ET AL., IET NANOBIOTECHNOLOGY, vol. 1, no. 3, June 2007 (2007-06-01), pages 27 - 35
LU N. ET AL., J. AGRIC. FOOD CHEM., vol. 56, no. 23, 2008, pages 11526 - 11532
ASAMIZU, PMB, vol. 54, 2004, pages 405 - 414
HALBWIRTH; STICH, PHYTOCHEMISTRY, vol. 67, 2006, pages 1080 - 1087
RAO S.; ROSSMANN M., J. MOL. BIOL., vol. 76, no. 2, 1973, pages 241 - 56
SATO S. ET AL., DNA RESEARCH, 2008, pages 1 - 13
TANAKA Y; FUKUCHI-MIZUTANI M; MASON JG: "Rose Encyclopedia", 2003, ELSEVIER, article "Cloned genes", pages: 341 - 347
See also references of EP 2615169A4
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
Kobayashi 浩 (JP)
Kobayashi 浩 (JP)
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