Title:
FLUID DEVICE, EXOSOME ANALYSIS METHOD, BIOMOLECULE ANALYSIS METHOD, AND BIOMOLECULE DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2015/045666
Kind Code:
A1
Abstract:
The present invention provides a fluid device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, with which a series of flows can be used to even analyse exosome content by introducing a sample into the device. This fluid device (1) detects biomolecules included in exosomes in a sample, and is characterized by being provided with: an exosome purification unit (2) provided with a layer modified with a compound having hydrophobic chains and hydrophilic chains; a biomolecule purification unit (3); a biomolecule detection unit (4); a first passage (5) connecting the exosome purification unit (2) and the biomolecule purification unit (3); and a second passage (6) connecting the biomolecule purification unit (3) and the biomolecule detection unit (4).
Inventors:
ICHIKI TAKANORI (JP)
KOBAYASHI MASASHI (JP)
HAYASHI AYAKO (JP)
TSUCHIYA SHOICHI (JP)
AKAGI TAKANORI (JP)
UENO TARO (JP)
FUNATSU TAKASHI (JP)
MIYAMOTO KENJI (JP)
KOBAYASHI MASASHI (JP)
HAYASHI AYAKO (JP)
TSUCHIYA SHOICHI (JP)
AKAGI TAKANORI (JP)
UENO TARO (JP)
FUNATSU TAKASHI (JP)
MIYAMOTO KENJI (JP)
Application Number:
PCT/JP2014/071422
Publication Date:
April 02, 2015
Filing Date:
August 14, 2014
Export Citation:
Assignee:
UNIV TOKYO (JP)
NIKON CORP (JP)
NIKON CORP (JP)
International Classes:
C12M1/34; C12M1/00; C12Q1/68; G01N33/53; G01N37/00; C12N15/09; C12N15/113
Domestic Patent References:
| WO2012170037A1 | 2012-12-13 |
Foreign References:
| JP2008513022A | 2008-05-01 |
Other References:
TAKANORI ICHIKI: "Micro RNA ni yoru Gan Shindan Platform", GENDAI KAGAKU, March 2013 (2013-03-01), pages 42 - 45
M ASASHI KOBAYASHI ET AL.: "Development of the isolation method for exosome in human serum immobilized by biocompatible anchor for membrane", JSAP SPRING MEETING KOEN YOKOSHU, vol. 60 TH, March 2013 (2013-03-01), pages 12 - 194
MASASHI KOBAYASHI ET AL.: "Development of evaluation method for immobilized exosomes in human serum on the glass substrate modified with biocompatible anchor for membrane", JSAP AUTUMN MEETING KOEN YOKOSHU, vol. 74, 17 September 2013 (2013-09-17), pages 12 - 155
TAKANORI ICHIKI: "Diagnostic Platform of Exosomes in Body Flood", CELL TECHNOLOGY, vol. 32, no. 1, 2012, pages 91 - 95
AKAGI, T. ET AL.: "Immobilization and Isolation of Exosomes Using Polyethylene Glycol-Lipid- Modified Surface in a Microchannnel and Evaluation by Atomic Force Microscopy", INTERNATIONAL CONFERENCE ON MINIATURIZED SYSTEMS FOR CHEMISTRY AND LIFE SCIENCES, vol. 16, 2012, pages 1291 - 1293
M ASASHI KOBAYASHI ET AL.: "Development of the isolation method for exosome in human serum immobilized by biocompatible anchor for membrane", JSAP SPRING MEETING KOEN YOKOSHU, vol. 60 TH, March 2013 (2013-03-01), pages 12 - 194
MASASHI KOBAYASHI ET AL.: "Development of evaluation method for immobilized exosomes in human serum on the glass substrate modified with biocompatible anchor for membrane", JSAP AUTUMN MEETING KOEN YOKOSHU, vol. 74, 17 September 2013 (2013-09-17), pages 12 - 155
TAKANORI ICHIKI: "Diagnostic Platform of Exosomes in Body Flood", CELL TECHNOLOGY, vol. 32, no. 1, 2012, pages 91 - 95
AKAGI, T. ET AL.: "Immobilization and Isolation of Exosomes Using Polyethylene Glycol-Lipid- Modified Surface in a Microchannnel and Evaluation by Atomic Force Microscopy", INTERNATIONAL CONFERENCE ON MINIATURIZED SYSTEMS FOR CHEMISTRY AND LIFE SCIENCES, vol. 16, 2012, pages 1291 - 1293
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
Masatake Shiga (JP)
Masatake Shiga (JP)
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