Title:
FLUORESCENCE DETECTION DEVICE, AND FLUORESCENCE DETECTION METHOD USING THE DEVICE
Document Type and Number:
WIPO Patent Application WO/2012/008258
Kind Code:
A1
Abstract:
[Problem] To provide a fluorescence detection device which can produce measurement results having reduced fluctuations and can achieve the detection of an analyte with high accuracy and a fluorescence detection method using the device.
[Solution] A fluorescence detection device in which excited light is ejected from a light source to release an evanescent wave from a reflection surface, thereby exciting a fluorescence substance that labels an analyte immobilized on the reflection surface, and the excited fluorescence is detected by a light detection means, and a fluorescence detection method using the device. The fluorescence detection device and the fluorescence detection method using the device are so adapted that a chip structure comprising a reflection surface and a reaction layer formed on the reflection surface is attached to or removed from a main body of the device upon use or is fixed on the main body of the device upon use, wherein the reaction layer comprises a flow path and a reaction area which is formed in the flow path and has a ligand for capturing the analyte, and wherein the relationship between the size of the reaction area formed in the flow path and the size of an excitation area which is an area to be irradiated with the excited light for exciting the fluorescent substance that has labeled the analyte captured in the reaction area are so defined as to fulfill the following relationship: the reaction area > the excitation area.
Inventors:
AOKI, Youichi (INC. 1, Sakura-machi, Hino-sh, Tokyo 11, 〒1918511, JP)
青木 洋一 (〒11 東京都日野市さくら町１番地 コニカミノルタテクノロジーセンター株式会社内 Tokyo, 〒1918511, JP)
青木 洋一 (〒11 東京都日野市さくら町１番地 コニカミノルタテクノロジーセンター株式会社内 Tokyo, 〒1918511, JP)
Application Number:
JP2011/063687
Publication Date:
January 19, 2012
Filing Date:
June 15, 2011
Export Citation:
Assignee:
KONICA MINOLTA HOLDINGS, INC. (1-6-1, Marunouchi Chiyoda-k, Tokyo 05, 〒1000005, JP)
コニカミノルタホールディングス株式会社 (〒05 東京都千代田区丸の内一丁目６番１号 Tokyo, 〒1000005, JP)
AOKI, Youichi (INC. 1, Sakura-machi, Hino-sh, Tokyo 11, 〒1918511, JP)
コニカミノルタホールディングス株式会社 (〒05 東京都千代田区丸の内一丁目６番１号 Tokyo, 〒1000005, JP)
AOKI, Youichi (INC. 1, Sakura-machi, Hino-sh, Tokyo 11, 〒1918511, JP)
International Classes:
G01N21/64; G01N21/05; G01N21/27; G01N33/543
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (Gotanda Yamazaki Bldg. 6F, 13-6 Nishigotanda 7-chome, Shinagawa-k, Tokyo 31, 〒1410031, JP)
Download PDF:
Claims: